不灭性

It was found that the vaccines used did not cause the adverse reactions such as hypersusceptibility and changes of feed intakes, and that 2 times of vaccinations of the vaccines all effectively enhanced the antibody levels, respectively, in which the No.Ⅲ vaccine stimulated antibody as rapid as the first vaccination was done while the No. I and No.Ⅱvaccines did not result in the antibody by the first vaccination. It was concluded that the No.

结果发现，三种供试的高致病性蓝耳病灭活疫苗均未引起供试猪发生过敏反应和采食量变化等不良反应；三种供试灭活疫苗经2次免疫后，均能提高抗体水平，其中供试疫苗Ⅲ在首次免疫后即能有效产生抗体，而供试疫苗I和供试疫苗Ⅱ在首次免疫后不能有效产生抗体。

Results Before and after the two inactivators exerting,all the env gene variation was indistinctive,necle otide homogeneity was all 98%.

结果 两种方法灭活前后，包膜基因变异均不显著，核苷酸同源性均为98%。

Protease treatment of the plasma membranes could abolish the binding but NaIO_4 and glycosidase could not, indicating that nsLTP144 bound to plasma membranes protein without carbohydrate moiety. Using the homobifunctional cross-linking regent bissuberate (BS~3) and rice plasma membranes incubated with ~(125)I-Trx-nsLTP144, we identified, after SDS-polyacrylamide gel electrophoresis and autoradiography, a putative protein receptor on the rice plasma membranes with the molecular mass around 60 kDa. NsLTP144 can not trigger extracelluar alkalization in arabidopsis, but can abolish the extracellular alkalization effect of phytopathogen elicitor cryptogein, suggesting that cryptogein and nsLTP144 may bind to the same membrane protein. In vitro pull-down assay showed that nsLTP144 interacted with OsCaM1, a possible extracellular calmodulin, implying that nsLTP144 and OsCaM1 could function in the same signal transduction pathway. These results shed light on revealing the roles of nsLTP in vivo and make it promising to finally characterize the plasma membranes receptor of nsLTP.

发现~(125)I-Trx-nsLTP144、~(125)I-Trx-nsLTP110与水稻细胞质膜均具有特异性结合，而且结合是饱和性的、可被竞争的，符合配体-受体结合的典型特征，同时用于对照实验的蛋白质~(125)I-Thioredoxin没有此特性，表明水稻细胞质膜上存在nsLTP的受体；利用可氧化糖基的NaIO_4和水解糖基的N\'-糖苷酶F处理水稻细胞质膜，再进行结合实验，结合活性几乎不受影响；而利用胰蛋白酶处理细胞膜则使得结合能力几乎完全丧失，表明其受体为没有经过糖基化修饰的蛋白质；利用交联剂BS~3交联配体一受体后，再进行SDS-PAGE分离和放射自显影，结果显示水稻细胞质膜上的nsLTP受体中有一个60kDa的蛋白质可以与nsLTP144发生特异性的结合，可能是其受体；细胞外碱化实验表明，nsLTP144不能促使拟南芥原生质体细胞培养液的细胞外碱化反应，却能猝灭来自植物病原菌的激发子Cryptogein刺激拟南芥原生质体产生的细胞外碱化反应，表明nsLTP和Cryptogein结合细胞膜上相同的位点，保护了植物细胞免受Cryptogein导致的细胞程序性死亡，并诱导系统获得性抗性的产生；体外Pull-down实验表明，nsLTP144和水稻的OsCaM1具有相互作用，暗示了nsLTP144和OsCaM1可能同在一个信号通路上起作用。

In- tramedullary nailing does not require the same high level of patient compli- ance, but an externalfixator may be particularly useful for patients with vascular injury or extensive soft-tissue damage and contaminationThe endosteal blood supply is preserved to a greater degree with un- reamed nailing than it is with reamed nailing.

髓内钉不需要患者有高度依从性，而外固定架在患者有血管损伤或广泛软组织挫灭，污染时特别适合。

The effect of imposed sterility and culling on the reproductivity and population size of Ratlike hamster in enclosures was studied by using surgical operation. This study aims to testify two modeling predictions: fertility control can achieve similar result as simple culling, and sterility of both sexes can achieve an extra result in reducing reproductivity of hamsters. The key factors in population regulation of the hamster were also discussed.

本文采用结扎不育的方法研究了不育和&灭杀&对围栏内大仓鼠实验种群繁殖力和数量的作用，主要验证模型的两个预测：不育能够达到灭杀的效果；双性不育优于单性不育，同时探讨了大仓鼠种群调节中的关键因子。

Abstract］ Objective For the purpose of scientific evaluation and improvement on unreasonable parameter of viral inactivation,the dynamics curve of time corresponding to effect by given viral inactivation factor treated to blood plasma product were analyzed.Methods Aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with pH 4, human immunoglobulin and human hepatitis B immunoglobulin for intravenous injection with pH 4/pasteurization,treatment on human fibrinogen and human coagulation factor Ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.The mean and standard deviation also coefficient of variation for virus survival titer in different time were stated, dynamics curve of virus inactivation were made and analyzed.

目的 分析血液制品特定灭活因子的时效动力学曲线，科学性地评价与改进不合理的病毒灭活参方法系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白蛋白采用巴氏消毒法，狂犬病人免疫球蛋白采用低pH常温孵放法，静注人免疫球蛋白、静注人乙肝免疫球蛋白采用低pH/巴氏消毒法；人纤维蛋白原、人凝血因子Ⅷ采用有机溶剂/去污剂与干热法灭活病毒的验证资料，统计3批样品取样点的残余病毒滴度均值、标准差与变异系数，制作灭活病毒动力曲线图并进行分析。

Methods Aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with pH 4, human immunoglobulin and human hepatitis B immunoglobulin for intravenous injection with pH 4/pasteurization,treatment on human fibrinogen and human coagulation factor Ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.

系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白作者：魏宪义，魏红，蹇远勇，陈海，刘文芳目的分析血液制品特定灭活因子的时效动力学曲线，科学性地评价与改进不合理的病毒灭活参数。

Abstract］ objective for the purpose of scientific evaluation and improvement on unreasonable parameter of viral inactivation,the dynamics curve of time corresponding to effect by given viral inactivation factor treated to blood plasma product were analyzed.methods aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with ph 4, human immunoglobulin and human hepatitis b immunoglobulin for intravenous injection with ph 4/pasteurization,treatment on human fibrinogen and human coagulation factor ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.the mean and standard deviation also coefficient of variation for virus survival titer in different time were stated, dynamics curve of virus inactivation were made and analyzed.results human albumin pasteurization inactivated vsv and sindbis virus,hrig low ph inactivated vsv and sindbis virus,hrig low ph inactivated hiv virus,ivig low ph/pasteurization inactivated hiv virus,ivig low ph inactivated hiv virus,hbig low ph/pasteurization inactivated vsv,sindbis and polio virus.conclusion suggestion about improvement on unreasonable parameter and standard of virus inactivation is made,cultivate target virus directly from final product by appropriate cell or method to monitoring viral safety of blood plasma product is advanced.

目的 分析血液制品特定灭活因子的时效动力学曲线，科学性地评价与改进不合理的病毒灭活参数。方法系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病毒，人血白蛋白采用巴氏消毒法，狂犬病人免疫球蛋白采用低ph常温孵放法，静注人免疫球蛋白、静注人乙肝免疫球蛋白采用低ph/巴氏消毒法；人纤维蛋白原、人凝血因子ⅷ采用有机溶剂/去污剂与干热法灭活病毒的验证资料，统计3批样品多个取样点的残余病毒滴度均值、标准差与变异系数，制作灭活病毒动力曲线图并进行分析。结果人血白蛋白巴氏灭活vsv与sindbis病毒，hrig低ph灭活vsv和sindbis病毒，hrig低ph灭活hiv病毒，ivig低ph/巴氏灭活hiv病毒，ivig低ph灭活hiv病毒，hbig低ph/巴氏灭活vsv、sindbis与polio病毒。结论建议改进病毒灭活验证标准与不合理的灭活参数；采用终产品样品通过合适细胞或方法直接培养目标病毒来有效监测血液制品的病毒安全性。

Methods Aimed at vesicular stomatitis virus, sindbis virus, human immunodeficiency virus, polioviruses, pseudorabies virus,encephalon-yocarditis virus; validate data of viral inactivated on pasteurization of albumin, human rabies immunoglobulin with pH 4, human immunoglobulin and human hepatitis B immunoglobulin for intravenous injection with pH 4/pasteurization,treatment on human fibrinogen and human coagulation factor Ⅷ with solvent/detergent and vapor heating at 100 ℃30 min were systemic regularized respectively.

系统性整理针对水疱性口炎病毒、黄热病毒、脊髓灰质炎病毒、伪狂犬病毒、脑心肌炎病毒、人类免疫缺陷病(本论文仅供参考，如需转载本文，请务必注明原作者以及转载来源：论文图书馆 www.lwlib.com)作者：魏宪义，魏红，蹇远勇，陈海，刘文芳目的分析血液制品特定灭活因子的时效动力学曲线，科学性地评价与改进不合理的病毒灭活参数。

But it only can use one time, after once puts on cannot again take off, because extinguished actually its witch collects the strength under its influence already to surmount its own limit, too crossed the exuberant flame finally to be able to burn oneself, when it is loosened once more, the technique in instantaneous will obtain certainly the big strength, but in after, also will lose his/her body for extinguished actually all abilities Constrains the word spirit: The light depends on the technique name to be able the reflective launch technique Pulls the star box: God of death aristocrat's symbol * Breaks the Asian space newly, in order to does not let the space which the present world and the corpse soul each other contact produces impractically in the traversing break the accident separately outside the samsara soul, paces back and forth is breaking, passes through year after year gathers in together, afterwards the soul can separate the memory and the energy, does for returns to the samsara to prepare.

不过它只能使用一次，一旦戴上后就不能再脱下来，因为灭却师在它的影响下其灵子收束力已经超越了其自身的极限，太过旺盛的火焰最终会烧伤自身，当它再次被解下时，术者将在瞬间得到绝大的力量，但在之后，也将失去其身为灭却师的所有能力压抑言灵：光靠术名就能反射性的发动术牵星箱：死神贵族的象征吧（朽木白哉头上的东西★新断界亚空间，为了不让现世与尸魂界彼此接触而产生的空间虚浮在穿越断界意外失散于轮回外的魂魄，徘徊在断界，经过长年累月聚集在一起，之后魂魄会分离出记忆和能量，做为重返轮回做准备

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。