不灭

And a fluorescence molecule beacon probe is designed between upstream and downstream priers, 5' end of probe can mark fluorescence reporter group FAM, and the 3' end markes fluorescence quenching group DABCYL, and 5' end and 3' end of the probe have 6 bases which can be reflected and complemented to make fluorescence molecule beacon probe do not give out fluorescence signal in free state.

在上下游引物中间设计一条荧光分子信标探针，探针的5'端标记荧光报告基团FAM，3'端标记荧光淬灭基团DABCYL，探针的5'端和3'端有6个碱基能反折互补，使荧光分子信标探针在游离状态下不发出荧光信号。

Are they merely giving positive replies just to keep this silly girl entertained and is currently niggering behind my back??

之前给予正面答复的人，现在还落得被我侮辱：我怀疑，他们是否给了正面回答只不过是不想灭这傻丫头的热情，而现在他们正在背后笑话我的单纯呢？

And the daughter said, Yes, mother, I did filter the tea leaves. But I couldn't find the tea strainer,so I used the fly swatter.

& 女儿回答说&是的，妈妈，我应该过滤了茶叶，但是我找不到漏洞，所以我用了灭蚊拍。

Or new crackling like a while, and we want to stare at the Sino-US coal stove, but less than five minutes the fire went out.

或新噼里啪啦想了一阵，我们中美希望地盯着煤炉，可是不到五分种火就灭了。

Compared to figleaf gourd, cucumber and sponge gourd showed higher net CO_2 assimilation, actual quantum yield of PSII electron transport Φ_(PSII, photochemical quenching coefficient, the efficiency of excitation capture by open PSII centers, rate of energy dissipation via light-independent thermal processes and fluorescence J_(f,D, energy flux via linear electron transport J_(PSII and electron flux for photosynthetic carbon reduction all the time during the measuring day.

黄瓜与丝瓜在净二氧化碳同化速率、光系统Ⅱ光化学量子效率Φ_(PSⅡ、光化学猝灭系数、开放的光系统Ⅱ中心激发能捕获效率、不依赖于光的热能与荧光耗散光能J_(f，D、PSⅡ总的光合电子流量J_(PSⅡ及用于光合碳还原的电子流量等参数上均表现出比黑籽南瓜更高的数值。

Our names are the light that glows on the sea waves at night and hen dies without leaving its signature.

我们的名字，便是夜里海波上发出的光，痕迹也不留就抿灭了。

Whose hope shall be cut off, and whose trust shall be a spider's web.

8：13 凡忘记神的人，景况也是这样。不虔敬人的指望要灭没。

The impact of three-stage magic is to eliminate lead to numerous, and ultimately also requested a coach (2 stage to please the coach) and finally passed, when the hunters have a question mark, colorful wizard (Aberdeen Cephalomappa pot experience I do not know the land reclamation no), to the fourth stage of relatively simple.

三阶段的影魔也是导致无数次的灭，最终还请了教练（2阶段也请了教练来），最后终于过了，当时猎人有问号，七彩精灵（肥牛火锅仔我不知道经历了开荒没），到第四阶段就比较简单了。

These programs shut down the motivation of all kids who aren't considered gifted and talented.

社会心理学家杰夫·霍华德指出：&这种制度浇灭了在它看来不具天分的学生的上进心，他们的自信心被摧毁了。&

Then ask ourselves the question: Can the Red Army with broadswords and long spears leaded by Chairman Mao defeat the western foreign devils?

试问：在毛主席领导下的大刀、长矛灭不了那几个杂毛西方洋鬼子？

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。