不溶血的

Use of highly haemolysed samples, incompletely clotted sera, samples with microbial contamination may give rise to erroneous results.

使用高度溶血的样品、凝血不完全的血清、含有纤维蛋白的血浆或微生物污染的样品可能引起错误的结果。

Morphological conversion from spiralto coccoid forms occurred when HP was cultured with selenium and most of coccoid forms had fragmentary flagella and pilus; the effection of selenium to activity of urease, catalase, alkaline phosphatase is not exist or not evidence; selenium enable pET32a-vacA-E.coli BL21 which can express VacA produce yellow lipochrome, and the color become deeper with increasing of concentration of selenium; selenium can decrease expression of recombined VacA by restrain the growth of pET32a-vacA-E.coli,but can not influence activity and gene sequence of VacA; selenium can enhance the suppression effect of recombined VacA for gastric cancer cell, depress the index of cell multiplication and the forming rate of colony, decrease the number of cell in S period, increase total of cell in G period; After purified, the recombined VacA can be identified by VacA antibody.

体外实验结果表明：一定浓度的硒作用HP后，HP菌落数量随着硒浓度的增加而减少，菌落颜色由灰白色变为砖红色，溶血现象随硒浓度的增加和作用时间的延长而逐渐消失，HP的形态由弯曲形变为球形或椭圆形，鞭毛及菌毛出现脱落现象；硒对尿素酶、过氧化氢酶、碱性磷酸酶活性无影响或影响不明显；一定浓度的硒能使表达VacA的pET32a-vacA-E.coli BL21工程菌产生脂溶性砖红色色素，颜色随硒浓度增加而加深；一定浓度的硒通过抑制pET32a-vacA-E.coli BL21工程菌的生长，减少重组VacA表达量，但不影响其空泡毒活性及编码基因序列；纯化后的VacA重组蛋白可被VacA抗体识别，具有良好的抗原性。

These two isolates presented typical characteristics of Streptococcus iniae with gram-positive cocci in chains, catalase negative and beta-hemolytic, growth under 10℃ and non-growth up 45 ℃, hydrolyzation of esculin and arginine, Voges-Proskauer, urease, and hippurate tests negative, fermentation of glucose, salicin, sucrose and starch, non-fermentation of arabinose, inulin, lactose, melibiose, raffinose and sorbitol.

实验结果表明，两菌株具有典型的海豚链球菌特性：在显微镜下为球形细胞，呈长短不一的链状排列，革兰氏染色阳性：在血琼脂平板上显示β溶血；1O℃生长，45℃不生长；接触酶阴性；水解七叶苷、精氨酸，VP试验、脲酶和马脲酸试验阴性；发酵葡萄糖、水杨苷、蔗糖和淀粉，不发酵阿拉伯糖、菊糖、乳糖、蜜二糖、棉子糖和山梨醇等。

Glucose-6-phosphate dehydrogenase (G6PD, E. C. 1. 1. 1. 49) is a house-keeping enzyme in all cells, and also is a key enzyme in the pentose phosphate pathway. G6PD deficiency, affecting 200～400 million people in the world, can cause chronic non-spherocytic hemolytic anemia, favism, drug-induced hemolytic anemia, neonatal jaundice and several infectious diseases. Its inheritance mode is an X-linked incomplete dominance.

葡萄糖-6-磷酸脱氢酶（Glucose-6-Phosphate Dehydrogenase，G6PD，E.C.1.1.1.49）是存在于所有细胞中的一种看家酶，也是磷酸戊糖途径的关键酶。G6PD缺乏症累及全球2～4亿人，引起慢性非球形细胞溶血性贫血、蚕豆病、药物性溶血、新生儿黄疸及某些感染性溶血，其遗传方式是X连锁不完全显性方式。

The hematolysis rate and biologic immunocompetence of PNS-5 were detected with hemolysis test and experiment of splenocyte proliferation respectively.

皂苷在高等动物的消化道中不被吸收，故口服无溶血毒性，但其注射剂应用于临床有很小的溶血性，故研究皂苷的溶血性对临床用药安全性评价有很大的作用。

To obtain the tervalent fusion toxin gene, three toxin gene fragments from three species of Vibrio parahaemolyticus, Vibrio vulnificus and Vibrio mimicus were connected with the flexible linker using overlap extension PCR. The three toxin gene fragments respectively encode the mature proteins of the thermostable direct hemolysin of V. parahaemolyticus, the cytotoxin of V. vulnificus and the heat-labile hemolysin of V.

采用柔性Linker和重叠延伸PCR方法将三个毒素基因，即副溶血性弧菌的耐热型直接溶血素基因tdh、创伤弧菌的溶细胞毒素基因vvhA和拟态弧菌的热不稳定型溶血素基因vmhA的成熟蛋白基因片段进行串联，得到三联融合毒素基因tdh-vvhA-vmhA。

A large blood volume could be obtained by abdominal aorta approach, which leads to less haemolysis and no hurt to organs, no gas embolism or haemostasis caused by inappropriate operation.

腹主动脉采血法适用于取血量大的实验，不易溶血，不损伤器官，不会出现因操作不当造成的气栓与瘀血，有利于病理组织学检查。

A large blood volume could be got by abdominal aortic approach, which with less haemolysis and not hurt organs.

腹主动脉采血法适用于取血量大、不易溶血，不损伤器官，不会出现因操作不当造成的气栓与瘀血，有利于病理组织学检查。

But it seems there is another point which will not only watch the relationship between the hemolysis, but also RH factor is positive or negative, if it is positive there is no problem, since most citizens are positive, if negative on the trouble spots , and also looking for good this blood type in advance, for fear of massive production, this type hard to find time to find on too late.

不过好像这其中还有一点不光是看会不会溶血的关系，还要看RH因子是阳性还是阴性的，如果是阳性的就没问题，因为大多数国人都是阳性的，如果阴性就麻烦点了，还要事先找好这种血型，怕生产时大出血，这种血型很难找，到时候再找就来不及了。

Through this project, we have discovered that thermoliable haemolysin gene can be horizontally transferred between species. This new finding will overthrow the traditional viewpoint that the tl gene is Vibrio parahaemolyticus species specific.

通过研究，我们发现了不耐热溶血素tl基因可被水平转移的现象，从而推翻了目前所流行的tl基因是副溶血弧菌种类所特有的观点。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐