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Through this issue, we found that with 2% NaOH-NALC digested within 10 minutes in the rapid decomposition of the viscous sputum, in ensuring the effectiveness of digestion at the same time not too much because of the role of NaOH to kill bacteria (time to 20 minutes), and would not affect the role of magnetic particles; quantitative simulation of self-made through the application of clinical samples, we compared the acid-fast stain and auramine O staining of the detection limit, auramine O fluorescence staining against the backdrop of a clear dark green light acid-fast stain of Mycobacterium tuberculosis than the white background of the red eye-catching TB is more capacity, auramine O staining in the detection limit of 0.05 to 0.001 between Maxwell, higher than the acid-fast stain of Michael's 0.05 units, The former is more sensitive; In addition, the accumulation of magnetic nanoparticles of the samples after the smear, in the microscope show the number of TB was significantly higher than that of the specimens has not been enriched.

通过本次课题,我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质,在保证消化效果的同时不会由于NaOH过强的作用杀死细菌(时间控制在20分钟内),亦不会影响磁性颗粒的作用;通过应用自制定量模拟临床标本,我们比较了抗酸染色和金胺O荧光染色的检出限,金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目,金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间,高于抗酸染色的0.05个麦氏单位,前者敏感度较高;另外,经过磁性纳米颗粒富集作用后的标本涂片,在镜下显示其结核杆菌数量明显高于未经过富集的标本。

Experiment 2, an in-vitro study, was conducted to determine the dialyzability of phosphorus in different plant feedstuffs as experiment 1, with phytase supplementation (500 FTU/kg) and no phytase, respectively, and to research the relationship between the dialyzability with no phytase and apparent or true phosphorus digestibility.

试验2采用体外透析法研究了不同植物饲料(待测植物饲料同试验1)在添加植酸酶(500FTU/kg)和不添加植酸酶情况下磷的体外透析率,并探讨了不同植物饲料在不添加植酸酶情况下磷的透析率与其体内表观消化率和真消化率间的相关关系。

Observe the effect of Fuling Gancao Decoction on gastric liquid emptying and content of nitric oxide and acetylcholine esteras in the gastric fundus,antrum and duodenal tissu of functional dyspepsia rats and investigate the mechanism.1 Reference reviewThe reference review discussed the source of disease name of TCM for FD,etiology and pathogenesis of TCM,selection of therapeutic methods by different stages or according to the differential syndrome diagnosis,advance on the clinical and experimental research as well as the problems lying in the research of TCM and corresponding strategies.We also have summarized the recent research progresses on the FD.In the meanwhile,we further exploited the pathologic characteristics,diagnosis and treatment of FD;summarized etiology and pathogenesis of TCM for FD and application of Classical Prescriptions in clinical and experimental study;Collected and arranged the Literatures and summarized the recent research progresses of Fuling Gancao Decoction;Finally, collected and arranged the literatures of proved cases and clinical experiences of Fuling Gancao Decoction.2 Theoretical studyThe Theoretical study first discussed the source of TCM for stomach deficiency and congestion of fluid-retention in syndromes of FD;Secondly, summarized the indications of Fuling Gancao Decoction,that is:①epigastric throb,②cold hands and feet in syncope,③hydroadipsia,④gastric blockade,⑤gastric splashing sound,⑥no desire for drinks,⑦light white tongue,thin-white and smooth fur,⑧deep and wiry pulse are the indications of Fuling Gancao Decoction;Subsequently,analyzed the composition of Fuling Gancao Decoction,considered that ginger is the main drug which treating congestion of fluid-retention in Middle-Jiao by Zhang Zhongjing.3 Experimental study3.1 Experiment oneObjective:To establish the animal model of FD with delayed gastric liquid emptying and evaluat it.

同时观察茯苓甘草汤对FD大鼠胃液体排空及胃底、胃窦及十二指肠中乙酰胆碱酯酶、一氧化氮含量的影响,探讨茯苓甘草汤的作用机制。1文献综述参照古、今中医有关文献对FD的中医病名、病因病机、辨证论治方法、临床及实验研究进展进行了探讨,并对现代医学在FD研究中所取得的(来源:A0bBC论文网www.abclunwen.com)进展进行了总结,进一步明确了FD的发病机制、诊断和治疗的研究进展;总结了中医对FD的病因及发病机制、中医诊断及经方治疗FD临床及实验研究进展;并搜集和整理已发表的有关茯苓甘草汤研究报道,阐述了茯苓甘草汤的现代研究进展;最后搜集和整理了近现代著名医家应用茯苓甘草汤的经验及验案。2理论研究理论研究部分首先对功能性消化不良胃虚饮停证的认识进行了理论溯源;其次对茯苓甘草汤的适宜证象进行总结,认为:①心下悸,②四肢不温,③口不渴,④胃中痞满,⑤胃中振水音,⑥不欲饮水,⑦舌淡白,苔薄白而滑,⑧脉沉弦是茯苓甘草汤的主证;随后对茯苓甘草汤方药组成解析,认为生姜是仲景治疗中焦饮停的一味主药。3实验研究3.1实验一目的:探讨建立功能性消化不良胃虚饮停证的动物模型方法并对其进行评价。

Symptom : the main function of the small intestines is absorbing the nutriment and transport the food leftover to the bowel, the main function of bowel is excretion and have no digestion function , if the contraction and ballooning of the colon appeared ,it will raise bugling ,poor digestion ,abnormal excretion and no molding stool, when it over-expand; after defecating you want to defecate again, so the chronic toxin can easily deposit in the colon and the diverticulun is formed.

症状:小肠的主要功能为吸收养分,吸收完后的食物残留物则输送至大肠,而大肠本身没有消化的功能而主司排泄,所以当肠道出现紧缩或扩张的现象时,就会引起胀气,消化不良,排泄不正常或大便不成型,扩张过度时亦会造成排完便后仍有便意,也容易造成肠道壁有慢性毒素沉积而产生憩室与宿便的囤积。

For example, can't we imagine an alternative history in which physiologists discover that the humans don't do any digestion themselves--rather, our stomachs contain insects that digest our food for us, excreting waste products which are exactly what we need to live.

例如,我们难道不能设想一个可能的情况,生物学家发现人本身不能消化,胃里的一些微小生物消化着我们的食物然后排泄出来的才是我们活命所需要的?

If it is swallowed in large masses it cannot be masticated at all, and will have but a small chance of being digested; and in an undigested state it will prove injurious to the stomach and to the other organs concerned in digestion, by forming unnatural compounds.

如果是在大型群众吞噬不能咀嚼所有,并有一个小机会,但被消化;和在未消化国家将证明损害胃和其他有关机关的消化,形成自然的化合物。

Also with light tea alternate feed. 4, if necessary, go to the hospital drips, add water, electrolytes and glucose, to prevent dehydration and acidosis. 5, breast milk contains more lactose, most lactose enzyme decomposition of absorption and less by the Department at the end of fermentation to digest the lactose, producing gas, acid production, stimulate the intestinal tract, so that stool was diarrhea-like discharge. 6, breast and milk contain fat, part of being broken down into fatty acids and glycerol absorption, not alkaline digestion of fat in small granular form of calcium soap, so that stool can not appear in the first one to digest things.

还可与淡茶水交替喂。4、必要时去医院打点滴,补充水分,电解质和葡萄糖,以防脱水和酸中毒。5、母乳中含乳糖较多,大部分被乳糖分解酶分解吸收,少部末被消化的乳糖发酵、产气、产酸、刺激肠道,使大便呈腹泻状排出。6、母乳和牛乳均含有脂肪,一部分被分解为脂肪酸和甘油吸收,未被消化的脂肪碱性的钙形成小颗粒状的肥皂,使大便中出现消化不掉的一颗一颗的东西。

The digestions should be less than 6 times, and every digestion should last for less than 20 min. Two-hour differential adherence could improve the purity of myocardial cells. By this protocol, 94.6% of cultured cells were live myocardial cells.

分离细胞时消化次数最好控制在不多于6次,每次消化时间不超过20min.2h差速贴壁可以提高心肌细胞纯度,经肌动蛋白鉴定,心肌细胞达94.6%。

Should be no problem, as long as your baby to eat good, sleep good, do not usually cry when tried, and tried very law, there would be no problem, but now add the milk of many types of nutrients, your baby is too small , incomplete digestion can not be completely absorbed, you may have to happen, the mother can also point to the baby to feed,"a Health fat" is what we often say that the yeast film, there are powder, and good for feeding a baby, my baby is also small, I am fed on improved several times, this is mainly to promote digestion, and no side effects, can be assured.

应该没有什么问题的,只要宝宝吃的好,睡的好,平时便便时不哭闹,而且便便很有规律,就没有什么问题的,现在的奶粉里添加的营养成分种类太多,宝宝太小,消化功能不完善,不能完全吸收,可能会有这种情况出现(有时有绿色的,还有灰白色的),妈妈也可以给宝宝喂点&胖得生&也就是我们常说的酵母片,也有粉状的,对于小宝宝好喂食,我家宝宝小的时候也有这种情况,我喂过几次就有所改善了,这个主要是促进消化的,没有什么副作用,可以放心。

Through this topic, we discover with the 2%NaOH-NALC digesting fluid may in 10 minute in rapid decomposition sputamentum resisting medium, during guarantee digestion effect not because the NaOH strong function will have killed the bacterium (timing control in 20 minutes), also will not affect the magnetic pellet the function; Through the application self-restraint quota simulation clinical specimen, we have compared the acid-fast stain and the pyoktanin yellow O fluorescence dye pick out limit, the pyoktanin yellow O fluorescence dyes the dark background to after-crop the obvious green bright the bacillus tuberculosis to compare in the acid-fast stain white background the red bacillus tuberculosis to accommodate strikingly, the pyoktanin yellow O fluorescence dyes picks out limits between 0.05~0.001 Mai unit, is higher than the acid-fast stain 0.05 Mai unit, the former sensitivity is high; Moreover, concentrates the function after magnetic nano particles the specimen smear, under the mirror demonstrated that its bacillus tuberculosis quantity is higher than the specimen which obviously has not passed through concentrates.

通过本次课题,我们发现用2%NaOH-NALC消化液可在10分钟内迅速裂解痰液的粘性介质,在保证消化效果的同时不会由于NaOH过强的作用杀死细菌(时间控制在20分钟内),亦不会影响磁性颗粒的作用;通过应用自制定量模拟临床标本,我们比较了抗酸染色和金胺O荧光染色的检出限,金胺O荧光染色黑暗背景下发出明显绿色亮光的结核杆菌较抗酸染色白色背景中的红色结核杆菌更容醒目,金胺O荧光染色的检出限在0.05~0.001个麦氏单位之间,高于抗酸染色的0.05个麦氏单位,前者敏感度较高;另外,经过磁性纳米颗粒富集作用后的标本涂片,在镜下显示其结核杆菌数量明显高于未经过富集的标本。

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