不染色细胞

Results:(1) Progesterone-treated models showed intact nasal mucosa, regular ciliary lining and inactive glands;(2) Untreated or normal saline treated models showed disrupted mucosa, inverted cilia and massive mucosal infiltration of neutrophils;(3) Smearing of nasal discharge revealed limited vs abundant number of neutrophils in SD models treated with progesterone vs untreated or treated with normal saline.(4) Hoechst stain: significantly fewer apoptotic cells per field were found in progesterone-treated models (1.583 ± 0.28) compared with untreated or normal saline treated models (2.85 ± 0.285 and 4.8 ± 0.715, respectively).

结果：(1)用黄体酮滴鼻液治疗的SD大鼠鼻腔黏膜上皮完整，纤毛整齐，腺体开放不多；(2)用或不用生理盐水滴鼻液治疗的SD大鼠鼻腔黏膜上皮纤毛倒伏，上皮结构散乱，上皮层大量中性粒细胞浸润；(3)鼻腔分泌物涂片示黄体酮滴鼻液治疗的SD大鼠有少许中性粒细胞，而用或不用生理盐水滴鼻液治疗的SD大鼠鼻腔分泌物含有大量中性粒细胞；(4)Hoechst染色：用黄体酮滴鼻液治疗的SD大鼠鼻腔黏膜上皮凋亡细胞数［(1.583±0.28)/视野］明显少于用或不用生理盐水滴鼻液治疗的SD大鼠［分别是(2.85±0.285)/视野和(4.8±0.715)/视野］。

The results showed that with treatment of the fixative solution added with antimonite,the free Ca2+ in pear different organs and tissues could be changed into antimonite calcium and precipitated in situ,which,after being stained,appeared as black particles under electronic microscope,whereas such particles could not be observed in the treatment without antimonite.

结果表明：在固定液中加焦锑酸钾处理进行制样，梨树不同器官或组织细胞中的自由态Caa2+，都能生成焦锑酸钙在原部位沉淀，经染色后在电子显微镜下呈现出黑色颗粒，而固定液中不加焦锑酸钾处理的则没有这种颗粒。

Decalcification solution has no effect on the staining of mast cell in nasal mucus by histochemical staining and immunohistochemical staining.

不同脱钙液（8%盐酸脱钙液、EDTA脱钙液、混合酸脱钙液）处理不影响鼻腔粘膜中肥大细胞的组织化学和免疫组织化学染色。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, which indicates PX and melatonin supplementation have no significant effect on CRH levels.

结果： ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低，提示松果体摘除后下丘脑CRH 神经元的表达增强；②松果体摘除大鼠补充褪黑素后，下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复，不过不论是低剂量组还是高剂量组，也不论是术后4 周还是术后8周，均不能恢复至正常；③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低，提示下丘脑CRH 神经元释放CRH 增加；④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性，表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, whichindicates PX and melatonin supplementation have no significant effect on CRH levels.

结果： ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低，提示松果体摘除后下丘脑CRH 神经元的表达增强；②松果体摘除大鼠补充褪黑素后，下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复，不过不论是低剂量组还是高剂量组，也不论是术后4 周还是术后8周，均不能恢复至正常；③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低，提示下丘脑CRH 神经元释放CRH 增加；④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性，表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

Strain YT-7 was Gram positive,nonmotile,non-sporing and long rods.

该菌株细胞呈长杆状，不运动，不形成芽孢，革兰氏染色阳性。

Six weeks after implantation, small amount of oval lacunae appeared. PGA fibers began to be absorbed. The amount of collagen increased and Safranin O stained positively. Eight weeks after implantation, cartilage with single or occasionally double chondrocytes in round lacunae was found.

体内埋植4周后可见PGA纤维间被不成熟的软骨细胞充填。6周时出现椭圆形软骨陷窝，PGA纤维开始吸收，胶原增多，番红花—"O"有阳性染色。8周时软骨陷窝呈圆形，单个，双个对称或多个排列。

Methods: The expression of apoptosis and the positive expression of p53, bcl－ 2 proteins were observed in 31 cases of breast cancer in situs, 20 breast dysplasia and 20 breast hyperplasia by using DNA nick end labeling technique and immunohistochemical staining . Eight normal breast tissues were used as control.

利用DNA缺口末端标记技术和免疫组织化学染色，原位观察31例乳腺癌，20例乳腺不典型增生和20例乳腺单纯性增生中细胞凋亡和p53、bcl－2蛋白的表达，以 8例正常乳腺组织作为对照。

Findings as follows: Experiments 1: Hormone to ovocyte IVM mature ratio influence: Dyes with H33342 observes COCs in three kind of different culture environment FSH → without hormone,FSH→ LH, FSH + LH → without hormone.""→""

研究结果如下：试验1：用H_(33342)染色观察COCs在将卵母细胞分别在三种不同培养环境中FSH→不含激素、FSH→LH、FSH+LH→不含激素。&→&

Epilepsy rat models were made by injecting KA into amygdale under stereotactic instrument.The ERK1/2 protein expression in various groups were tested with immunohistological method.

采用立体定位仪下杏仁核内注射KA方法制备大鼠癫痫模型，于致痫后不同时程进行灌流固定、心肌组织的石蜡包埋、切片及免疫组织化学染色，检测不同时程心肌细胞ERK1/2表达的灰度值。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。