不染色的

As the DDP group A549DDP cells were treated with IC10 of cisplatin for 48 hours. As the DDP＋Rh2 group A549DDP cells were treated with IC10 of cisplatin and IC5 of gensenoside Rh2 for 48 hours. As the controlling group A549DDP cells were not treated with any kinds of drugs. Changes in cellular morphology were observed by fluorescence microscopy. Peak value apoptosis of A549DDP cells were inspected by flow cytometry. State of PTP was evaluated by ultraviolate spectrofluorometer. Changs of calcium in cells and membrane potential of mitochondrion were determined by flow cytometry. Release of cyt-c from mitochondrion with westton blot Radiative activity of〓Tc-MIBI ingested by cells was measured withγcounter. Results: IC50 of cisplatine to A549 cells was 24uM.

以无毒浓度的Rh2单独作用于A549DDP细胞作为Rh2组，以低效浓度的顺铂单独作用于A549DDP细胞作为DDP组，以无毒浓度的Rh2和低效浓度的顺铂联合作用于A549DDP细胞作为Rh2+DDP组，作用时间为48小时，以不加药物干预作为对照组，Hoechst33258染色荧光显微镜下观察细胞形态的变化，流式细胞仪检测细胞凋亡峰；紫外光分光光度计检测线粒体PTP开放情况，流式细胞仪检测细胞内钙离子浓度及线粒体跨膜电位的变化，Westton blot检测线粒体内细胞色素C释放情况及Caspase-3的活性，γ计数仪检测细胞摄取〓Tc-MIBI放射性活性。

Simultaneously 15 samples of normal nephric tissue were used as control.The sections from these samples were subjected to immunohistochemical analysis with antibodies directly against uPA and PAI-1 by LSAB method. In this study we adopt semiquantitative analysis to determined the expression of these molecules , and utilized the relevant data processing and statistical analysis to evaluate the correlation between their expression and the clinicopathological features of RCC.Results: 1. The positive rate of uPA and PAI-1 of uPA in RCC were 72%(36/50)and 56%(28/50), respectively.But in control group there was nearly

正常肾组织标本中几乎无阳性染色。2、uPA表达水平与患者年龄、性别、肿瘤病理分级无关(均P＞0.05)，与T分期、淋巴结转移有明显的相关性(r=0.684，P＜0.05；r=0.789，P＜0.05)，uPA高表达多见于T_(3-4)、有转移的RCC.3、PAI-1的表达水平与患者年龄、性别、肿瘤病理分级和T分期无关(P＞0.05)，与淋巴结转移有明显的相关性(r=0.800，P＜0.05)，PAI-1高表达多见有转移的RCC.4、对uPA和PAI-1行相关性检验，uPA在RCC中的表达与PAI-1的表达呈明显正相关(r=0.168，P＜0.05)。5、uPA和PAI-1的表达与淋巴结转移的关系以Logistic回归法进行分析，结果显示uPA与区域淋巴结转移密切相关，uPA是淋巴转移的危险因子(RR=2.718，95％CI=1.150～7.170，P=0.024)，PAI-1则不是淋巴转移的危险因子(RR=1.599，95％CI=0.624～4.102，P=0.328)。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。