不成熟

By the adult,says is the moral wants the good .but foundation of the moral wants the good faith,this point as if is the vouth who soon good faith,this point as if is the youth who soon marches into the society ,natural.by maturing,resembled studies this degree,should be successful,what but faced was a broader ignorance,studied,but not deep,knew but not fine,the innermost feelings,went without saying apprehensively.by industry,as if only then achieves the adult you only then to be possible.to complete,no matter and matures or not but you must achieve want the adult,however the adult with the relations which matures,did not need to speak the archaism to have the cloud:the pre-advance its matter ,must benefit first its,the adult with matures is also so,if wants to mature you to result in the first adult.

由成人，说是道义上要好的，但基础的道德希望的诚意，这一点，如果是vouth谁尽快诚意，这一点，如果是青年谁尽快游行，融入社会，自然。由成熟，类似于研究这种程度，应该是成功的话，什么，但面对的是更广泛的无知，研究，但不深，知道但不罚款，内心的感受，去说apprehensively.by业，如果只有这样，实现了成人只有这样，你要possible.to完成，无论是和成熟，或没有，但你必须要实现成人，但成人与关系日趋成熟，并不需要讲古有云：前提前事，必须有利于它的第一，成人与成熟，也因此，如果要成熟，你的结果，在第一成人。

Vg could be detected in haemolmph of the water—fed female at 48h after eclosion, too.

用10％的蜂蜜和清水分别饲喂两组成虫(简称"蜜组"和"水组")，以研究成虫期的营养对Vg发生和卵巢发育的影响，结果显示，成虫期营养对于Vg的启动合成并不起关键的决定性作用，两组成虫均可以在羽化的48小时检测到Vg，且水组中的血淋巴水平并不低于蜜组对照，但绝大多数水组个体的卵巢发育停留在卵黄沉积的某一时期，此时在血淋巴中也检测不到，只有约20％的饲水个体卵巢可以发育成熟，出少量卵；用高剂量的ZR515于羽化当日点滴处理饲水个体，可以使所有接受处理的饲水个体的卵巢发育成熟，但是产卵〓依旧低于对照组的，不能恢复正常；这说明成虫期营养对卵巢中全体卵母细胞的成熟是必需的，其作用的产生是通过内分泌系统途径实现的。

Following chemical activation, blastocysts rate of the treated oocytes was similar to untreated oocytes.8 Following fertilization, however, few oocytes inhibited with CHX developed into morulae/blastocysts, due to a high incidence of polyspermy.9 Cortical granule migration occurred during inhibition, but CHX inhibition impaired CG migration, significantly no oocytes inhibited by CHX completed CG migration after maturation.10 CHX inhibition had no effects onα-microtubles and microfilaments of goat oocytes.

抑制24h转为正常培养24h，不影响卵母细胞的成熟和孤雌激活能力，并且CHX抑制后再成熟的卵母细胞经孤雌激活发育到囊胚的比例与对照组卵母细胞相似。8、体外受精后，CHX抑制后再成熟卵母细胞的多精受精现象显著增加，发育到桑椹胚/囊胚的比例显著低于对照组。9、CHX抑制过程中皮质颗粒仍能发生迁移，但是CHX抑制会对皮质颗粒的迁移造成不可恢复的损伤，使再成熟的卵母细胞内皮质颗粒不能完全迁移。10、CHX抑制对山羊卵母细胞α-微管和微丝都没有影响，无论是抑制后处于生发泡期的卵母细胞，还是抑制后再成熟的卵母细胞，微管和微丝的分布都与对照卵母细胞相似。

This two species of Gloiopeltis could mature and release spores at illuminance 2 500~8 500 lx. For no nutrient addition to the stocking water the two species of Gloiopeltis could mature and release spores, while nutrient addition too high (80 mg·L-1 NaNO3 and 8 mg·L-1 KH2PO4) it was not good for the mature and spores release of this two species alga.

结果表明，在水温10~20℃培养条件下，海萝能成熟放散孢子；在水温12~24℃时，鹿角海萝能成熟放散孢子；在光照2 500~8 500 lx的范围内，2种海萝均能成熟放散孢子；培养海水中不添加营养盐对这2种海萝的成熟没有影响，培养液的浓度高达硝酸钠80 mg·L-1、磷酸二氢钾8 mg·L-1时反而不利于藻体的成熟及孢子放散附着。

The results show that: 1 supplementation of protein to maturation media improves cumulus expansion in vitro compared to the protein-free control, but cumulus expansion is not necessarily related to oocyte nuclear maturation in pigs, and cumulus expansion is not the criterae for determination of nuclear maturation of pig oocytes, but only the exclusion of the first polar body; 2 exposure of pig COCs to hormone supplements for 23-24 hours improved cumulus expansion but had no significant effect on nuclear maturation compared to that for 46-48 hours; 3 under our research conditions, supplementation of different proteins into different maturation media has different effects on porcine oocyte nuclear maturation, but has no significant effect on subsequent embryonic development after IVF; 4 the nuclear maturation rates of pig oocytes matured in mTCM+pFF and mNCSU+pFF are superior than that in mNCSU+FCS; 5 different maturation media have no effect on pig oocyte cumulus expansion and subsequent embryonic development after IVF.

结果显示：(1)在成熟液中添加蛋白质可以加强卵丘细胞的扩散，但猪卵母细胞的核成熟与其周围的卵丘细胞扩展没有必然的联系，卵丘细胞扩散或成放射状不宜作为猪卵母细胞核成熟的标准，只有排出第一极体才能作为猪卵母细胞核成熟的标志；(2)在猪COCs的46-48小时成熟培养的后23-24小时阶段去除成熟液中的激素不但可以保证卵母细胞的核成熟率，而且可加强卵丘细胞的扩散；(3)在现有实验条件下，在mTCM和mNCSU中添加10％pFF与在mNCSU中添加10％FCS相比可获得较高的猪卵母细胞核成熟率；(4)在不同的成熟液中添加不同的蛋白质对猪卵母细胞核成熟率的影响效果不一样，但对体外受精后的早期胚胎发育影响不明显；(5)成熟液种类对猪卵母细胞的卵丘细胞扩散和体外受精后的早期胚胎发育无显著影响。

Pig oocytes cultured in vitro for some time were inseminated by frozen–thawed ejaculated sperm. At specified times after insemination, sperm penetration, cell cycle progression and mitogen-activated protein kinase phosphorylation were evaluated. It was shown that:(1) oocytes at various maturational stages could be penetrated by sperm;(2) sperm penetration did not affect meiotic cell cycle progression;(3) sperm penetration of germinal vesicle oocytes and maturing oocytes did not alter MAPK phosphorylation; and (4) when premetaphase I and metaphase I oocytes, in which MAPK was activated, were fertilised, no evident MAPK dephosphorylation was detected as in metaphase II oocytes.

不同成熟阶段的猪卵与精子融合后体外培养发现：(1)不同成熟阶段的猪卵母细胞都可被精子穿透；(2)精子穿透不影响减数分裂细胞周期进程；(3)精子穿透GV期卵母细胞和正在成熟的卵母细胞不改变MAPK磷酸化：(4)已受精的前中期I和中期I卵母细胞的MAPK有活性，MAPK不像MII卵母细胞那样受精后发生磷酸化。

Japonicus.Nerve extraction substance of sea star and dithiothreitol has been reported to be effectively to induce oocyte maturation in sea cucumbers. In this study we tested their effects on oocyte maturation induction in sea cucumber A. japonicus. The results are as follows, oocyte of A. japonicus does not get mature spontaneously. Neither NES alone nor NES with follicle suspension can induce oocyte maturation. While DTT can prominently increase the percentages of germinal vesicle break down. When the concentration of DTT is between10-1 mol/L and 10-3 mol/L, the effect of maturation induction is more sufficient. When the concentration is about 10-2 mol/L, the percentage of GVBD can reach up to more than 90%.

分别利用在海参中有诱导作用的海星神经提取液和二硫苏糖醇对从成熟卵巢解剖出的刺参卵母细胞进行诱导成熟试验，结果显示，刺参的卵母细胞在海水中不会发生自发成熟，海星神经提取液以及神经提取液加滤泡悬浮液对卵母细胞没有诱导成熟的作用，而二硫苏糖醇处理可以诱导卵母细胞生发泡破裂，当DTT溶液的浓度处于10-1 mol/L到10-3 mol/L之间时，对刺参卵母细胞的促熟作用较为明显，在10-2 mol/L左右时，卵母细胞的诱导成熟率可以达到90%以上。

In part this was due to early partners reporting on her past inexperience, but also due to reports of her death, such as the 1998 news item from Outside which stated that Mauduit and her partner, Ang Tsering Sherpa,"either failed to dig their tent out from under a cover of fresh snow or neglected to turn off their camp stove."

这部分是由于她早期的合作伙伴披露了一些她过去不成熟的做法，还有关于她的死因的一些报道，例如1998年一些新闻说起Mauduit和她的搭档Ang Tsering Sherpa时说，&他们或者是搭帐篷时未躲开新雪，或者是忘记了熄灭帐篷里的炉子。&

"I don't think this is ready for prime time," said Dr. Jonathan S. Bromberg, director of transplantation at Mount Sinai Hospital in Manhattan.

&我认为时机还不成熟，&曼哈顿西奈山医院器官移植主任乔纳森·S·布隆伯格博士说。

"I don't think this is ready for prime time," said Dr. Jonathan S. Bromberg, director of tra lantation at Mount Sinai Ho ital in Manhattan.

&我认为时机还不成熟，&曼哈顿西奈山医院器官移植主任乔纳森·S·布隆伯格博士说。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。