不完全变态中的

To investigate whether the expression of cdc2 and cyclin B1 in spermatogenic cells during spermatogenesis is actually a temperature dependent event, in situ hybridization, Western blotting and immunohistochemistry analysis were used to study the expression of cdc2 and cyclin B1 in normal and cryptorchid testis. Results showed that heat would differentially hurt male germ cells in different developmental stages during spermatogenesis, especially the pachytene primary spermatocytes. Most of spermatogonia in contralateral cryptorchid testis were not harmed fatally by heat as yet, indicating that spermatogonia could resist to beat to a certain extent. In this case spermatogonia could develop to pachytene/diplotene primary spermatocytes, but they could not acquire the ability to complete the transition from mitosis to meiosis, and then appeared to go through apoptosis. Therefore, we could not find the descendants of meiosis: secondary spermatocytes and round spermatids, elongated spermatids and spermatozoon. The abdominal temperature had no significant influence on the transcription of cdc2 and cyclin B1 in the spermatogonia and pachytene/diplotene primary spermatocytes. In normal rabbit testis, cyclin B1 increased in the pachytene/diplotene primary spermatocytes before meiosis and reached its peak in the spermatids.

为了解精子正常发生过程中cdc2和cyclin B1表达的低温依赖性，我们利用原位杂交和免疫组化等方法，研究了正常和隐睾精子发生过程中cdc2和cyclin B1的转录和翻译调控活动，结果表明：（1）热对各阶段的雄性生殖细胞都有损害，粗线期的初级精母细胞尤为敏感，实验性隐睾内的精原细胞尚未完全受到"致命"影响，说明精原细胞对热有一定的耐受性，但即使成为粗线期／双线期初级精母细胞，却未能获得由有丝分裂过渡到减数分裂的能力，呈现不同程度的凋亡，所以在整个切片中找不到源自减数分裂的产物----次级精母细胞、圆形精子细胞，更谈不上长形精子细胞和精子的形成；（2）腹腔高温未明显地影响隐睾精原细胞和粗线期/双线期初级精母细胞中cyclinB1和cdc2的转录，说明高温并不是通过影响cyclin B1和cdc2的转录活动而导致生精过程阻断的；（3）正常兔睾丸组织中，〓在精原细胞和粗线期／双线期精母细胞中均有表达：cyclin B1蛋白在减数分裂前期的粗线期／双线期初级精母细胞中的表达量增加，于变态末期的精子细胞中达高峰。

I still remember a cold evening in 2003's winter,when the snowflake was blusterous in the sky,and when the road was much pale owning to the ice on the ground, and I had only two choices in front of me,going abroad or pursuing a postgraduate degree,maybe others didn't know my suffering,but I did,for my English was so poor that whichever I chose ,it would not be avail for me at last,I participated in a series of English classes,reading and writing all day long,I was very sorry that I had troubled too many friends, as a result ,I could simply communite with our foreign professor,but the problem was not simple,I got a low mark in my Toefl test,thanked to the language class in the school abroad,I arrived at Quebec three monthes later,my first impression about this city was cold, since my hometown is located in the south of the Yangtze River, I have to imagine what does the 'heavy snow' mean cause there are always snowing lightly,to my surprise,almost half a year it was heavy snowing in the city,and it did really satisfy my desire about the snow,furthermore,we also had a long holiday,when I got there,most of my classmates were prepared for their vacation ,so I could asked for help from the senior, maybe I was not tall but sometimes the snow on the ground was taller than me,actually ,I had a good time when I stayed with my friends for we have a common language named chinese,the language class was simple for me,but the teacher was serious,whether you passed the course or not was depended on her, so you'd better don't offend her,thank godness, I passed this class at first time after a few monthes,then began with the other courses,it was said that chinese students abroad was the most assiduous in the world,and that was ture,usually,we didn't have a strong suit in the ability of a foreign language,since it asked for some understanding in our course,so I had to borrowed the note from others when I first went to class,how times fly,my GPA first was above 4.0 at that time,I phoned my relations,telling them I was okey there,but in the first evening of that holiday,a fire broke out in our dorm,I couldn't find my passport after the incident,though most of our res were moved outside,maybe it was burnt out or was lost at somewhere,but it told me that I couldn't stay longer in this city,we comforted each other for a while,''Goodbye!

雪花在2003年一个寒冷的夜空中无情的肆虐着，回家的大道被雪映的煞白，而摆在我面前的却只有两条窄窄的小路，考研，出国，也许别人不会知道我的痛苦，可是我清楚，其实我的英语很烂，现在不管选那个，好像对我都不利，我疯狂的去参加一Qy系列的英语辅导班，从早到晚大声的阅读，没天没地的做题目，以致招来了很多非议，感觉可以和外教简单交流了，可是我的托福成绩还是不堪入目，不过好在那边学校开了语言课，所以三个月后，我还是顺利来到了加拿大的魁北克省，那里给我的第一感觉就是一个字'冷'，我们江南一般下小雪，大雪是什么只能靠想像了，不过那边将近有大半年的时间都在下大雪，让我这个雪盲好好过了一把雪瘾，不过假期也长，我去的时候大多已不上课了，所以可以找到学姐，学兄，也许是我的个子不太高，不过积雪有时完全可以把我湮没，和他们在一起感觉很亲切，很开心，也许我们在一起都说汉语吧，语言课是比较简单的，不过老师很变态，她说行才行，不行你就是行也不行，所以这个老师是绝对不能得罪的，学了很长一段时间，语言考试通过了，就可以正常上课了，呵呵，都说中国留学生最刻苦了，不苦行么，我们语言能力一般都不是强项，加上课程需要一些理解的能力，初次去听课，不借阅他人的笔记是绝对不行的，时间总是晃的很快，那次的GPA成绩第一次超过了4.0，打了电话回家，报了平安，可是假期的第一个晚上就发生了一些小小的意外，一把无名火把我们住的地方烧了，当时东西都搬出来了，好在没有损失什么，可是后来检查了一下，我的护照怎么都找不到了，也许是烧了吧，也是是掉了，反正这件事也告诉了我，魁北克我是待不长了，朋友们在一起相互惋惜了一阵。

In the previous work, we found a new antibacterial substance existed in foregut of an Oxya chinensis, an incomplete metamorphosis insect.

本实验首先以不完全变态的直翅目昆虫中华稻蝗为材料，从中华稻蝗的前肠提取液中检测到了抗菌物质的存在。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐