不同地

After thecomplete genome extraction of the strain was performed, the genomic DNA was partiallydigested by restriction enzyme Sau3AⅠ, the DNA fragments from 1 to 5Kb was clonedinto prokaryote expression vector pET-28a-c, and transformed host bacteria. The resultsshowed that we succeeded in constructing the gene expression library of haemophilusparasuis serovar 5, which is fundamental for the study of advanced gene screening. Inaddition, primer design was performed based on haemophilus influenzae in this study. In addition, PCR was performed by using genomic DNA of haemophilus parasuisserovar 5 as the template. The results demonstrated that we obtained two neo-gene:23SrRNA gene(conserved gene belonging to the large-subunit of ribosome) and adenylatecyclase gene(encodes adenylate cyclase and participates in converting adenyl nucleosidetriphosphate to cyclic adenosine3",5"-monophosphate). Furthermore, the phylogeneticanalyses between the species was performed, and neighbor-joining tree was constructedbased on comparison of 23S rRNA gene sequences, so it was illuminated betweenHaemophilus parasuis and other species in molecular evolution relationship.

选择我国流行优势菌株副猪嗜血杆菌血清5型地方株为研究对象，提取细菌基因组DNA，用限制性内切酶Sau3AⅠ对基因组DNA进行部分酶切，回收大小为1～5Kb的DNA片段，将其连接入原核表达载体pET-28a-c，最后转化宿主菌，结果成功地构建了基因表达文库，为后续的基因筛选工作奠定基础；另外，本研究选择嗜血杆菌属的流感嗜血杆菌为参考对象进行引物的设计，以副猪嗜血杆菌血清5型地方菌株的基因组DNA为模板，进行PCR扩增反应，结果表明成功地获得两个新基因：23S rRNA基因(存在于核糖体大亚基中的保守性基因)和腺苷酸环化酶基因(负责将腺嘌呤核苷三磷酸转变为环腺苷酸)，并进一步做了不同物种之间的分子系统发育分析，构建了基于23S rRNA基因的邻接法系统发育树，阐明了副猪嗜血杆菌与其它菌种的分子进化关系。

After the initiation", Sri Ramakrishna once said, describing the event,"Nangta began to teach me the various conclusions of the Advaita Vedanta and asked me to withdraw the mind completely from all objects and dive deep into the Atman.

开始之后&，室利罗摩克里希纳曾经讲述过这件事：&Nangta开始教导我不二论吠檀多的各种不同结论，要求我把头脑完全地从所有对象中撤离，深入地进入阿特曼。

There were 22 pollinating insect species belonging to 4 orders and 11 families, among which, native bee species Megachile abluta, M. spissula, Xylocopa valga, Andrena parvula and Anthophora melanognatha were the main pollinators. M. abluta, M. spissula and X. valga were of uni-peak type with diurnal foraging activities trom 11:30 to 15:30, while A. parvula and A. melanognatha were of double-peak type with diurnal foraging activities on 9:30-11:30 and 16:30-18:30, indicating a complementary relationship among the pollinators. The main pollinators had significant differences in their tripping frequency and landing time, and their foraging behaviors were correlated with their body sizes.

结果表明：苜蓿访花昆虫共计22种，分别属于4个目，11个科，根据弹花效率初步确定鳞地蜂、黑颚条蜂、净切叶蜂、细切叶蜂和紫木蜂为河西地区主要传粉昆虫；主要传粉昆虫的日活动规律出现单峰型和双峰型2种，净切叶蜂、细切叶蜂和紫木蜂属于前者，只在11：30-15：30出现一个活动高峰，而鳞地蜂与黑颚条蜂则为双峰型，在9：30-11：30和16：30-18：30分别出现访花高峰，不同的传粉昆虫之间存在互补关系；主要传粉昆虫的弹花频率和小花停留时间存在显著差异，且访花行为与野生蜂体型紧密相关。

There are two different world between work with passion and steadily and work apathetically .

激情而投入地工作与麻木而呆滞地工作是完全不同的两个天地。

Could it possibly be that Harry's place is not to destroy LV and the DE's in a final, apocalyptic duel of archetypical proportions, but to, rather, destroy what is at the core of their evil ways: hatred and mistrust of those who are different.

有没有可能，哈利并不是要在最后的、毁灭性的、有着人类最原始本性的斗争里，击垮伏地魔和他的食死徒，而是，相反地，销毁他们邪恶的根源本身：对不同人群的憎恨与猜疑。

The result indicated that good symbiosis was formed between AM fungi and Avena sativa, and different sample sites significantly affected the colonization of different structure and spore density of AM fungi.

对青海省民和、大通、湟中、海北4样地燕麦根际AM真菌空间分布和土壤成分测定分析表明，燕麦可与AM真菌形成共生关系，AM真菌不同指标和孢子密度与样地生态条件密切相关。

Software Description: About Awave Audio, This program allows you to perform batch audio conversions between 90 different audio file formats.

Awave Audio 这个程序允许你迅速地和容易地执行进到超过 30 种不同音频的格式的一批音频信号转换。

Therefore,some sports prescription that takes advantage of extracurricular sports activities are brought forward to contrapose different types of psychologi...

因此，我们提出针对大学生不同的心理疾病类型，发挥课外体育活动的优势，以人为本地制定促进大学生心理健康的运动处方，从而更好地发挥运动处方针对性强、灵活性强的特点，为全面落实体育与健康课程的改革提供有力的帮助。

The wind corrade of differ underlying surfaces' research express that in bottomland , the erosion of no-tillage of vegetable land is the most, it is 504.02 g/m2, the second is the stubble land of corn and plough land, the stubble land of naked oats.

对不同下垫面的风侵蚀量的监测表明，滩地类型下免耕菜地的风蚀量最高，达504.02g/m~2，其次是玉米留茬地和耕翻地，莜麦留茬地风蚀量最小。

After analysis of hydrocarbon to different kinds of ore, rock and inclusion, it is discovered that organic materials had been more or less participated in the mineralization process of Au, Ag, Sn, Pb, Zn, Cu, Sb deposits (include some mid-high temperature deuteric hydrothermal deposits). At the same time, higher content of hydrocarbon has been well preserved to present, even more than tens thousands uμl/kg.

首次系统地对各类矿石、岩石、矿物气液包裹体等进行了烃类组分分析，结果发现，各种金、银、锡、铅、锌、铜、锑等金属矿床(包括岩浆期后中高温热液矿床)的成矿过程均不同程度地存在有机物的参与，至今仍存在很高的烃气含量，甚至高达几万μl/kg。

Recall that, in the Kolmogorov axiomatization, measurable sets are the sets which have a probability or, in other words, the sets corresponding to yes/no questions that have a probabilistic answer.

延期开工的Kolmogorov宣布要衡量所有套职能有限许多坐标限制在于衡量子寅。换句话说，如果是/否的问题回答f可看的价值观念在许多最有限坐标，然后它有一个概率的答案。

The experimental results showed that the algorithm can filter singular points and remain fixity of the target edge.

实验表明：基于模糊理论的图像智能滤波方法比常规图像滤波方法运算量小，它既能去除奇异点，又能保持图像中目标的边缘不变性，具有很好的滤波效果。