不可分离的

Low resolution was obtained in genetic variation investigation of different cowpea cultivated germplasm of Vigna unguiculata based on ITS sequence data, while good results could be obtained in genetic diversity and phylogeny of differern species of genus Vigna.

RAPD可以用于种下的遗传多样性研究。但基于随机引物的RAPD-PCR扩增，受实验中各种因素的影响较大，导致数据获得的重复性不是很好、且不可移植。因此只能作为一种辅助方法。从技术上讲，为保证实验条件的一致性，应该一次PCR反应中对所有的研究对象进行扩增，并在扩增片段的电泳分离检测时尽量保持条件的一致。

Linton complied; and had he been unrestrained, would probably have spoiled all by filling his epistles with complaints and lamentations: but his father kept a sharp watch over him; and, of course, insisted on every line that my master sent being shown; so, instead of penning his peculiar personal sufferings and distresses, the themes constantly uppermost in his thoughts, he harped on the cruel obligation of being held asunder from his friend and love; and gently intimated that Mr Linton must allow an interview soon, or he should fear he was purposely deceiving him with empty promises.

林惇顺从了；如果他不受拘束，他大概会使他的信中充满了抱怨和悲叹，结果就会把一切搞糟：但是他的父亲监视他很严；当然我主人送去的信每一行都非给他看不可；所以他只好不写他特有的个人痛苦和悲伤，而这是他的思想里最先想到的题目，他却只表达了硬把他与他的朋友和爱人分离之苦；他还向林惇先生慢慢暗示必须早些允许见面，不然他会担心林惇先生是故意用空话来搪塞他了。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。