不加入

In this paper the changes of Si/C and CE and alloy addition on the mechanical properties and microstructure of gray cast iron were studied.

通过CE和Si/C量的变化，并分别加入与不加入合金，探讨了它们对灰铸铁性能和组织的影响。

Denmark twice raised interest rates in October to help protect its currency, the krone, which is pegged closely to the euro.

即便是像前社会主义国家波兰那样坚持不加入欧元区的国家也在加速准备加入欧盟。

In Norway, when the German-controlled premier Vidkun Quisling tried to get all teachers to join a political union, 12000 out of 14000 resigned rather than do so.

在挪威，德国控制的总理维德昆。吉斯林曾要所有教师加入一政治联盟，结果一万四千个教师，有一万二千个宁愿辞职都不加入。

Then the cultivated chondrocytes were embedded in fibrin glue fused on spongy bone, covered with priosteal flap; the complex was used to repair the femoral trochlea osteochondral defect which size is 3mm × 4mm × 4mm made in rabbit knee joint.

在A组的每只兔子的一侧膝关节股骨滑车部人为造成3mm×4mm的骨软骨缺损，骨刀切除软骨下骨到髓腔渗血为止(厚约4mm)，压迫后FG止血；取髂骨骨块，并尽可能保留松质骨，取下的骨块用PBS反复清洗，以除去血细胞，将松质骨填充在骨缺损处，松质骨面朝向关节腔，高度与周边软骨下骨齐平，把骨膜片生发层朝向关节腔，用无创伤缝合线缝合在周边的软骨或滑膜组织上；向EP管中加入1/2悬液体积的FG主体胶溶液并混匀，再与主体胶等体积的催化剂溶液一同注射入骨膜与骨块密闭的腔隙中；同理处理另一侧膝关节。B组处理与A组相比只是不加入软骨细胞；C组造成骨软骨缺损，FG覆盖创面后单纯用骨膜修复缺损。

Methods TASMCs were stimulated with LPS in the presence or absence of CCK-8 and/or PKC inhibitor chelerythrine, morphine,-OR mRNA expression was analyzed by RT-PCR 16h after stimulation.

培养大鼠TASMCs，在加入或不加入CCK-8和/或CHE、吗啡的情况下，用LPS刺激细胞16h，用半定量RT-PCR检测κ阿片受体mRNA的表达。

The disubstituted ureas and the catalyst could be separated by addition of water and the catalyst could be reused without deactivation.

新型高效的菲咯啉钯-离子液体含氮化合物羰化催化剂体系，以苯胺为反应物时该体系的转化频率可以达到6000mol/mol/h；氢氧化铯-离子液体催化剂体系，可以在不加入化学计量脱水剂的条件下活化二氧化碳与脂肪族胺反应得到二取代脲，反应结束后可以通过加入水实现产品和催化剂体系的分离，催化剂体系可以重复使用。

Cells in the induction group were incubated in 1.5 mL of osteoinductive medium, supplemented with 10-8 mol/L dexamethasone, 10 mol/L β-glycerophosphoric acid, and 50 mg/L vitamin C.

选取第3代骨髓间充质干细胞和脂肪间充质干细胞，按5×107 L-1接种后各分为2组：诱导组加入含10-8 mol/L地塞米松、10 mol/L β-甘油磷酸、50 mg/L维生素C的成骨诱导培养基1.5 mL，未诱导组不加入成骨诱导培养基。

METHODS: The third passage of BMSCs and AMSCs at a density of 5×10^7 L^(-1) were divided into two groups. Cells in the induction group were incubated in 1.5 mL of osteoinductive medium, supplemented with 10^8 mol/L dexamethasone, 10 mol/L β-glycerophosphoric acid, and 50 mg/L vitamin C. Cells in the non-induction group were not treated with osteoinductive medium.

选取第3代骨髓间充质干细胞和脂肪间充质干细胞，按5×10^7L^(-1)接种后各分为2组：诱导组加入含10^8mol/L地塞米松、10mol/L β-甘油磷酸、50mg/L维生素C的成骨诱导培养基1.5mL，未诱导组不加入成骨诱导培养基。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of MCSF (25μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 μg/L) and M-CSF (25 μg/L) with or without 10 μg/L TNF-α for4, 5, 6 and 9 days. The number of TRAP multinucleated cells and resorption pits on dentine slices were counted under light microscope.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞，在含有25μg/L M-CSF和0，1，10，100μg/L TNF-α的α-MEM培养液中培养5d后，观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成；细胞在含有25μg/L M-CSF和30μg/L sRANKL的α-MEM培养液中进行培养，比较加入和不加入10μg/L TNF-α培养4、5、6和9d后，所形成的TRAP多核细胞的数目和骨吸收面积。

Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of M-CSF (25 μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞，在含有25μg/LM-CSF和0，1，10，100μg/LTNF-α的α-MEM培养液中培养5d后，观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成；细胞在含有25μg/LM-CSF和30μg/LsRANKL的α-MEM培养液中进行培养，比较加入和不加入10μg/LTNF-α培养4、5、6和9d后，所形成的TRAP多核细胞的数目和骨吸收面积。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失