不分离的

You can say i'm selfish, ignorant and that i don't care gotta understand i took your pain away from your own fears putting you through this would shatter your hope and dreams you just don't see it right now till it hits your own scene believe me i care it's just that this world is all about survival dealing with denial it's our own personal issue and trial Chorus 2x Only in deep down dreams i feel your face as i stare off into the lost open space or ratherly pounds of thousands pace concentration gets hard when i'm at work mixed up between dreams and reality, yes it hurts i find myself hiding just so i can feel ya true love, i never achieve it so loneliness still attached to my everyday instinct so many new faces dashing through been searching forever just tryna find the real you ah, imagine you and i at that place, that place that separates pain away from peace in the country field we dash through just ,you and i as we join hands and hands spread your wings and sooth with me to another land over the ocean as we freeze through the breeze these memories will not be put to rest learn to move on and live your life at it's best forward towards another day waking up stunned again damnn, i'm just dreaming away...

你可以说我自私，无知，而且我也不在乎爱是理解我把你的疼痛远离自己的担心让你通过这将打破您的希望和梦想你刚才没有看到它现在直到访问您自己的舞台相信我，我照顾只是，这个世界就是生存处理拒绝这是我们自己的个人问题和审判合唱团2 只有在内心深处的梦想我觉得你的脸我瞪了进入了开放空间或ratherly磅的数千步伐浓度会很难当我在工作混合了梦想与现实之间，是疼我发现自己隐藏，所以我能感觉到亚真正的爱情，我从来没有做到这一点如此孤独仍然附在我的日常本能这么多新的面孔通过破灭一直在寻找永远只是tryna找到真正的你啊，你可以想象，你和我在那个地方，那个地方分离疼痛远离和平在该国的领域，我们通过破折号刚才，你和我，因为我们携起手来，双手展开你的翅膀和抚慰我到另一个陆地的海洋，因为我们通过冻结微风这些记忆将不会平息了解进入你的生活和居住在最好迈进新的一天起床震惊再次damnn ，我只是梦想远离找到了

The calculation results shows that ,(1) when the rotor rotates at the working speed, a local regurgitant whirlpool that is rightabout to the rotor"s rotating direction appears in the classifying region, which makes the separated granules come back to the outside of the classification district and influences the classification efficiency of the turbine classifier;(2) in the condition of a low rotating speed of turbo rotor, ultrafine particles can follow the gas phase by and large, but when the rotating speed increases, a phenomenon of velocity slippage between two phases appears evidently;(3) A operating parameter, air flow rate is so important that can directly influence the stability of flow field in classifying chamber, the bigger the air flowrate is , the steadier the flow field is;(4) Compared with straight blades, the new shape of blades with backward crooked elbow are helpful to improve the stability of flow field and form whole stream easily, also beneficial to advance the classification accuracy and classification efficiency;(5) The motion trace of particles are very complicated when the turbo rotor rotates at a high speed, the constant bounce of the particles appear between the blades and the coarse particles are even bounce back from the inlet, only the finer can pass through the classification area from the oulet;(6) Along with the increase of the rotor"s rotating speed, radiah axial and tangential velocity all begain to fluctuate repetitively like sine-wave, the smaller the particles are, the more violently they fluctuates.

数值模拟结果表明，(1)在涡轮达到工作转速时，在分级区域形成了一个与涡轮旋转方向相反的局部回流涡，它使得分离出来的细粒重又被带回分级轮外边缘，从而严重影响分级机的分级效率；(2)粉尘粒子在涡轮低速运动情况下，基本上能跟随气体运动，但随着转速加大，两相之间出现了明显的速度滑移现象；(3)分级机工作时的风量也是一个比较重要的操作参数，它可以直接影响到分级机内腔流场的稳定性，风量越大，流场越稳定；(4)同直叶片形状相比较，带后弯导板的叶片，有利于改善流场的稳定性，流场容易形成整体流，对提高分级效率和分级精度都非常有利；(5)粒子轨迹线在涡轮转速较高时十分复杂，有的出现了反复的弹跳现象，大颗粒不能通过分级区域而从进口返流了，只有细小的颗粒才从出口处流出；(6)涡轮转速加大时，粒子的径向、轴向和切向速率出现了明显的类似于正弦波的脉动现象，颗粒越小，脉动程度越为剧烈。

Total RNAs from KAx-3 cells and AK127 cells(developed for 14h) were isolated. After the reverse transcription and PCR reaction, two distinct differential fragments were acquired., fragment A was from KAx-3 cells and fragment C was from AK127 cells. After retriving and reamplifying the differentially expressed fragments, white-blue plaqueselection, the fragments were purified. Northern blot proved that fragment A was from KAx-3 cells and fragment C was from AK127 cells. The results of sequencing and researching for NCBI database have been showed: part sequence of fragment A shows 91% similarity to the gene encoding DhkA, 92% similarity to the gene encoding DhkF, 91% similarity to the gene encoding STATc, 97% similarity to the homoeobox gene encoding protein. These genes play important part in controlling cell differentiation and cell proportion in Dictyostelium discoideum.

本研究通过提取盘基网柄菌发育14小时的野生型KAx-3细胞和突变型AK127细胞的总RNA，运用mRNA差异显示法分离出了两条明显的差异表达片段，其中片段A来自野生型KAX-3细胞，片段C来自突变型Ak127细胞；并通过凝胶回收差异片段、对差异片段进行再次PCR、蓝白斑筛选克隆、提取质粒、酶切电泳纯化差异片段；接着进行Northern杂交的结果表明，片段A只与野生型KAx-3细胞的总RNA有杂交信号，片段C只与突变型AK127细胞的总RNA有杂交信号，这就排除了差异片段假阳性的可能；最后通过测序，搜索NCBI BLAST数据库发现：片段A的小部分序列与编码组氨酸激酶DhkA基因中一段序列的相似性高达91%，与编码组氨酸激酶DhkF基因中的一段序列相似性高达92%，与编码STATc蛋白基因的一段序列相似性达91%，以及与编码同源框蛋白的基因中的一段序列相似性达97%，这些基因在盘基网柄菌细胞分化和细胞比例调控过程中起着相当重要的作用，这些数据进一步说明了突变细胞不能完成发育的原因。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。