不分离的

Many tumor specific antigens such as mouse PlA, human MAGE, BAGE and GAGE families had been isolated during recent decade. PlA is a tumor specific antigen expressed by mouse mastocytoma P815. It is one kind of cancer-testis antigens and expresses in various mouse tumor such as mastocytoma P815, plasmoma J558 and fibrosarcoma Meth A but hardly in normal tissue except testis and placenta.

近十年来已经成功分离出多种鼠或人的肿瘤特异性抗原，如小鼠P1A基因和人的MAGE、BAGE、GAGE家族等。P1A为小鼠肥大细胞瘤P815所表达的肿瘤特异性抗原，它是一种CT抗原，在多种小鼠肿瘤如肥大细胞瘤P815、浆细胞瘤J558和纤维肉瘤Meth A等中表达，而在除睾丸、胎盘之外的正常组织中不表达。

However, three isomers are obtained when [3(5)-〓-5(3)-MeS]pyrazole or[3(5)--5(3)-MeS]pyrazole reacts with 〓 in the same conditionpossibly owing to the influence of both the steric and electronic factors.

通过配体与〓的光化学反应，首次分离得到了不对称取代的双吡唑烷VIB金属配合物，但3位取代基的立体位阻减弱了双吡唑烷与〓的反应。

"Rampantly infected by 'the aesthetics of separation,' many people simply cannot think clearly, and before they have even really made a serious effort, they give up on their marriages, in the end suffering even greater regret and pain," says Chen. Marriage is not all or nothing, but is the art of compromise.

在泛滥的『分离美学』感染下，会让一些人在根本没想清楚、也还没有付出足够努力前，就糊里胡涂地放弃了婚姻，结果造成更大的遗憾和伤害，」陈艾妮强调，婚姻不是全有或全无的，而是一种妥协的艺术。

A midtone neutral gray is made up of approximately 50% cyan, 40% magenta, and 40% yellow, although this varies slighty with different scanners and separation software.

有的专业词汇你再改改，毕竟我不是搞这个的……中性灰是由大约50%的青色、40%品红、40%黄组成的，虽然用不同的扫描器和分离软件有细微差别。

However, opioid receptors are not expressed naturally in great abundance, they are relatively labile and frequently difficult to solubilize as monomers. The availability of their cDNAs makes them good candidates for overexpression in heterologous cells, which offers hope for the purification of membrane receptors for biochemical and structural studies.

在生物体内，阿片受体的含量很低，分离纯化过程中受体蛋白不稳定、易形成多聚体。cDNAs的克隆成功使阿片受体的异源高效表达成为可能，为研究阿片受体蛋白的生化特性和结构特征创造了条件。

For me that was: 0 Degrees: Asking a potential client if they want to hire me as a coach By performing complimentary sessions 1 Degree: Offering complimentary sessions by: Passing out cards at events Follow up autoresponders to those who clicked through website Speaking Networking Sending Out Articles Optimizing website Buy Google Ads Sending out autoresponders/ezines to clients with offers of co mplimentary sessions Talk/ write personal notes to clients 1 Degree Make offer more alluring Create, Update, an d Practice complimentary session Work on overcoming objections Focus on creating value for client 2 Degrees: C reating communication opportunities Finding speaking/radio gigs Finding networking gigs Writing articles Finding sites that accept articles Writing speeches Writing blogs Commenting on blogs Talking in discussion groups Promoting products 3 Degrees L ure potential clients Creating confidence in what I offer Creating products Improving products Spiffing up website Im proving copy Improving offer 4 Degrees Hone my abilities Take classes Receive coaching Coach Now, I couldn't do EVERY thing I've listed in 1 and 2 degrees of separation, I would be totally overwhelmed.

对于我来说，那是：0度：要求一个潜在的客户，如果他们想聘请我作为一个教练演艺义务兵1度：免费提供会议：●结业卡事件跟进autoresponders那些通过点击网站来说网络●寄出物品网站优化买谷歌广告派出autoresponders/ezines向客户提供合作mplimentary会议●谈/写个人笔记给客户1度，使提供更为惊人创造，更新，四实践相辅相成届工作，对克服异议重点放在创造价值，为客户度2：三砷通信商机●寻找发言/电台档期寻找网络档期●写文章寻找地点接受条款●写稿写作博客评论博客谈讨论组●促进产品3度1茜潜在客户建立信任，我所提供创造产品改进产品宾客最多的网站即时证明副本●改进提供400度锻炼我的能力班接受辅导教练，现在我不能做所有的事情我已经上市，在1日和2度分离，我会完全压倒。

METHODS: Bone marrow was sterilely separated from human. After heparinization, human BMSCs were harvested using density gradient centrifugation and adherence method. At the fifth passage, BMSCs at 1×108/L were incubated in the 6-well plate and divided into 2 groups. BMSCs in the edaravone group were 50% confluent and incubated in L-DMEM containing basic fibroblast growth factor and fetal bovine serum for 24 hours. After washing in PBS, these BMSCs were incubated in serum-free L-DMEM containing 20 mg/L edaravone for 24 hours. BMSCs in the blank control group were incubated in L-DMEM, supplemented with 10% fetal bovine serum.

无菌抽取的骨髓经肝素化后，采用密度梯度离心法及贴壁筛选法分离获得人骨髓间充质干细胞，传至第5代按1× 108 L-1接种于6孔板内，设立2组，依达拉奉组细胞达50%融合时用含碱性成纤维生长因子、胎牛血清的L-DMEM预诱导24 h，PBS洗涤后再用20 mg/L依达拉奉无血清L-DMEM诱导24 h；空白对照组始终用含体积分数为10%胎牛血清的L-DMEM培养，不加任何预诱导剂和诱导剂。

In the present study, we applied water-insoluble nickel oxide and nickel subsulfide to induce morphological transformation of human embryonic lung cells and to probe the differentially expressed genes related to the transformation.

因此本研究试图用两种水不溶性镍化合物诱发人胚肺细胞的恶性转化模型，并分离、克隆与之相关的差异表达基因，为深入研究镍致癌的分子机理提供初步的资料。

The cell adaptability tests showed that the CPE caused by wild CCV strains were different from reference strains syncytia CPE.

细胞适应性试验表明，分离到的野毒在A72细胞上导致的CPE不同于参考毒株的合胞体病变，NJ2、KM株主要导致细胞圆缩、脱落，NJ2株的6-15代在A72细胞上有CPE，但是15代后不再出现CPE。

We detected the distribution of lipases in 35 Aeromonas isolates by PCR andphenotypic test using tributyrin as substrate. The positive rates were 100% and 83%,respectively. The lipase activity or associated genes could be detected in the two avirulentstrains, A. hydrophila W1 and A. hydrophila 4332. It suggested that lipase might not be animportant factor in pathogenic mechanism of A.

对本实验室保存的35株气单胞菌分离株进行了脂酶的表型检测和PCR检测，结果分别为83％和100％，特别是嗜水气单胞菌无毒株W1和Ah4332也能检测到脂酶活性或基因，表明脂酶在该菌的致病机制中不是决定性因子。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。