不分离的

The recombinant plasmid pQEM1 could steadily exist in the X4064 strain of S. typhimurium,without influence on its invasion into host cells.X4060（pQEM1）strain isolated from infected mice still had the ability to re-express M1 protein.

重组pQEM1质粒能稳定地存在于减毒鼠伤寒杆菌X4064株中，不妨碍其入侵宿主细胞，从小鼠组织中分离到的X4064(pQEM1)依然具有表达M1蛋白的能力。

The methods to eliminate the furfural's interference in the determination of cyanide in wastewater, such as silver salt, petroleum ether extraction, two-step petroleum ether extraction, are tested and compared.

目前对糠醛干扰氰化物测定方面的报道不多，大多是只是针对醛类干扰的消除方法，主要有：NaCl -AgNO3-H2 SO4蒸馏法〔3〕、银盐沉淀法〔4 ，5〕、萃取分离〔6〕。

METHODS: After isolated the PBMCs and blood-plasma from adult human peripheral blood by Ficoll-Hypaque centrifugation, the PBMC culture procedure with or without the self-blood -plasma was applied to polarize T cells in vitro , these cells were polarized by PHA(20 mg/L),non-PHA respectively.

用Ficoll-Hypaque梯度离心法分离出正常成人抗凝外周血单个核细胞和血浆，将PBMC置于含或不含有自身血浆环境中，并在植物血凝素(PHA ，2 0mg L)的刺激下进行培养，培养 2 4、48、72h后收获细胞，分别用双色免疫荧光标记，以流式细胞术对CD4+ T细胞表达表面分子CCR3、CCR5进行分析。结果：CD4+ T细胞受自身血浆和PHA刺激后向Th1 Th2两个方向极化，2 4、48、72h后极化率分别达到(13 2 8± 1 5 9)%(12 70± 1 6 5 )%、(17 19± 1 0 3)%(17 5 0± 1 30 )%、(19 49± 2 87)%(18 5 8± 1 49)%；而只有PHA刺激的极化率很低，两组有显著性差异(P 0 1)；Th1∶Th2之比约等于 1。

High performance ion exchange chromatography has been demonstrated to be capable of efficient separation of bacteria of different species with high resolution on the base of the differences in both electrostatic charge property and structure of the cell.

高效离子交换色谱已被证明能根据细菌细胞的荷电性及表面构造快速而高分辨率地分离不同种属的细菌。

Root soils of Vitis vinifera cv. Cabernet sauvignon grapes of 4 and 8 years old in Yangling region vineyards were taken as samples; and the methods of plate culture and selective medium were used to culture and separate soil microbes. Then the microbes separated from different regions were counted and classified.

以陕西杨陵4年生和8年生赤霞珠葡萄的根系土壤为试验材料，利用平板培养法和微生物选择培养基进行培养分离，采用梯度平板涂布法计数，对葡萄根系土壤中的7种目标微生物（细菌、真菌、放线菌以及4种细菌生理群）在不同区域、不同层面的分布和数量进行比较、分析。

Mechanized placer mining, with special devices for washing and separating waste from valuable minerals, is used in productive alluvial deposits.

机械化砂矿开采用于含矿量丰富的砂矿床。利用专门设备来淘洗、分离废物与有用矿物。这个盆子是镀金金属的，所以不太值钱。

A suspension of finely divided particles in a continuous medium in which the particles are approximately5 to5, 000 angstroms in size, do not settle out of the substance rapidly, and are not readily filtered .

胶质在一连续介质中被细微分开的粒子形成的悬浮状态，粒子尺度约5至5，000埃，不会很快从该物质中分离，也不易被滤出

The soft rot of the Chinese cabbage in cellar develops throughout the storage period.

用病根及病叶等所作的堆肥中不能分离出具有致病力的軟腐細菌。

But it only can use one time, after once puts on cannot again take off, because extinguished actually its witch collects the strength under its influence already to surmount its own limit, too crossed the exuberant flame finally to be able to burn oneself, when it is loosened once more, the technique in instantaneous will obtain certainly the big strength, but in after, also will lose his/her body for extinguished actually all abilities Constrains the word spirit: The light depends on the technique name to be able the reflective launch technique Pulls the star box: God of death aristocrat's symbol * Breaks the Asian space newly, in order to does not let the space which the present world and the corpse soul each other contact produces impractically in the traversing break the accident separately outside the samsara soul, paces back and forth is breaking, passes through year after year gathers in together, afterwards the soul can separate the memory and the energy, does for returns to the samsara to prepare.

不过它只能使用一次，一旦戴上后就不能再脱下来，因为灭却师在它的影响下其灵子收束力已经超越了其自身的极限，太过旺盛的火焰最终会烧伤自身，当它再次被解下时，术者将在瞬间得到绝大的力量，但在之后，也将失去其身为灭却师的所有能力压抑言灵：光靠术名就能反射性的发动术牵星箱：死神贵族的象征吧（朽木白哉头上的东西★新断界亚空间，为了不让现世与尸魂界彼此接触而产生的空间虚浮在穿越断界意外失散于轮回外的魂魄，徘徊在断界，经过长年累月聚集在一起，之后魂魄会分离出记忆和能量，做为重返轮回做准备

According literature above,its considerd that the former is proper to be interpreted by means of multiple alleles hypothesis,and the fertility heredity of the latter is appropriate to be interpreted using inhibition.

根据大白菜及其近缘芸薹属作物核不育材料育成的纯合两型系和杂合两型系可育株间互交F~1|可育株自交，其子代可能出现无育性分离情况或者产生13∶3两种表型的育性比资料，认为前者宜用复等位基因假说解释，而后者用抑制作用解释为妥。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。