不分离的

In some cases, its epimer 4 was also obtained. 22 compounds were synthesized, among them 18 were new.3. Using the intramolecular Pictet-Spengler reaction to form the pentacyclic skeleton of Et-743, the byproducts 25 were obtained under various conditions. The structures of them were determined to be 1, 2-dihydro-isoquinoline compounds, and the mechanism of the reaction was proposed.B. The synthesis of the analogs of Et-743Because of the failure of removing the protective groups in compounds 14, the synthetic strategy was adjusted.

对于苄基保护的羟基化合物13a，顺利得到化合物30，然后与不同的羧酸反应得到18个酯化合物33-50；对于苄氧羰基保护的氨基化合物13b，由于脱除保护基的化合物31在空气中不稳定，所以我们采取不经分离纯化，直接与羧酸反应得到酰胺化合物53-62，然后再分别对其C-21位的羰基进行还原氰基化，得到10个酰胺目标化合物63-72，另外，我们还对羟基转化为氨基的反应进行了探索。3。

Making gfp fusion protein is a convenient method to mark the expression of interesting proteins, but it may interfere the expression of interesting proteins, since gfp is a quite big protein itself.

一方面，作为标记与目的蛋白融合可以方便的标示目的蛋白的表达情况，但gfp本身是个相当大的蛋白，会降低目的蛋白的表达；另一方面，进一步分离时需使用抗gfp的单克隆抗体进行免疫共沉淀，既不经济又不方便。

Findings from two experiments with infants with Williams syndrome (a phenotype selected to bolster innate modularity claims) indicate a within-syndrome double dissociation: For numerosity judgments, they do well in infancy but poorly in adulthood, whereas for language, they perform poorly in infancy but well in adulthood.

两个来自对带有Williams综合症的婴儿所进行的实验发现（一种被选出的显型以支持先天模组的主张），指出症状内双重分离：有大量的鉴定，他们在婴儿时期发展良好，但在成人时期则表现不佳；反而在语言方面，他们在婴儿时期表现不佳，却在成人时期发展良好。

The principle of harmonic wave detection which is based on wavelet transform was expatiated. It introduced a new approach of harmonic measurement based on period-interleaving of arithmetic for dissymmetrical system.

首先论述了基于小波变换的谐波检测的原理，根据目前小波变换在三相不对称系统谐波检测中存在的无法分离基波信号之不足，介绍了一种用于三相不对称系统的基于周期交错的测量方法。

For example, it is a pathogenic bacterium and has endotoxins; it secretes protein into the periplasm and often into inclusion bodies. Bacillus subtilis is an attractive host for the production of heterologous secretory proteins. Many genes has succeed expressed in Bacillus subtilis, and the plasmid vectors for Bacillus subtilis are now taken from other genus bacterium, such as Staphylococcus aureus or lactococcus lactis and so on. As it is clear to known that these plasmids are far from optimal for Bacillus subtilis, because of low efficiencies of cloning and high levels of replicating instability.

枯草芽胞杆菌是目前原核表达系统中分泌表达外源蛋白较理想的宿主，近几年已在枯草芽胞杆菌中成功表达了多种基因，但大多都应用来源于金黄色葡萄球菌、乳酸杆菌等的载体来表达外源基因，由于其在枯草芽胞杆菌中存在分离复制的不稳定及外源基因的克隆表达效率低的现象，故都不是枯草芽胞杆菌的最佳选择，还有待改进。

Under the windings of the sea They lying long shall not die windily; Twisting on racks when sinews give way, Strapped to a wheel, yet they shall not break; Faith in their hands shall snap in two, And the unicorn evils run them through; Split all ends up they shan't crack; And death shall have no dominion.

在海洋的卷之下他们横躺长的将不多风地死；当肌肉屈服的时候，在架上扭转，对一个轮子捆绑，然而他们将不打破；在他们的手的信心将咬断在二，而且独角兽邪恶穿越跑他们；在他们上面分离所有的结束而没有裂缝；而且死亡将没有领土。

In recent years, optically active polymers have attracted much attention because of their unique functions such as molecular recognition, optical resolution for racemates and catalytic activity in asymmetric transformations as well.

近年来，光学活性聚合物的合成与结构—性能研究深受国内外学者的高度关注，这是由于这些合成材料具有潜在的应用前景，如：光学活性聚合物可应用于外消旋混合物的对映异构体分离，即光学拆分过程；不对称吸附、手性诱导及许多有机化学反应过程中的不对称催化。

NO·1层: No girl 主 have to marry 谓 a man 宾 whose continue title have the alike initial as ITs ,定语语法 for, as the rhyme goes,"Change 状语语法 the title but not the letter, marry for worse instead of preferable."

要点 这是个主从复合句。for是连接词，引导状语语法。as the rhyme goes是插入语，插在for和它引导的语法之间，造成for和它引导的语法的分离，中文英语互译时，可译成唯一主谓框架。译文妞妞不该嫁给与本人的姓的首字母相同的人，因该正如一首童谣所说：改姓不改首字母，婚后必将受其苦。

Vandierendonck,A.,'The contribution of working memory resources in the verification of simple mental srithmetic sums',Psychological Research,1999,62,72-77.% q! Y! l*~# y: ^ [5] Miyake,A.,Friedman,N.P.,Emerson,M.J.,Witaki,A.H.

由于这两种任务只对中央执行系统产生干扰，对其他两个子系统不产生影响，因而能有效地分离工作记忆各子系统的功能，使研究更有效。

Methods TIL in the patients with PHC were separated with combinative enzymatic digestion of type Ⅰ,Ⅳ collagenases,type Ⅴ hyaluronidase and type Ⅰ deoxyribonuclease followed by discontinuous density gradients.Cytotoxicity of TIL to liver cancer cells was detected with MTT method.

采用Ⅰ型和Ⅳ型胶原酶、Ⅴ型透明质酸酶和Ⅰ型DNA酶四联酶消化，应用不连续密度梯度离心分离TIL，采用MTT比色法检测TIL对自体癌细胞及肝癌细胞株的细胞毒。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。