不分离的

The results showed that the virus isolation and identification could generate considerably reliable results but the procedure was complicated and costed long time; microtomy fluorescent antibody detection was fast, but the specificity was low and needed to exclude the false positive from the results. Sandwich ELISA detection combined the merits of the former two methods, but it generated the false negative results when the samples were in bad preservation. Compared with the others, RT-PCR was fast, high sensitive and especially useful for detecting the CSFV directly from the samples. The specificity could be raised by using the nested-PCR to detect the CSFV from the simples, especially when the simples were in bad preservation.

结果显示，病毒分离鉴定法准确性较高，诊断比较容易，但存在试验步骤繁琐，所需时间长的缺点；荧光抗体检测法所需时间较短，但需要经验比较丰富人员来判定结果，且存在假阳性结果，特异性比较差；RT-PCR检测法具有快速、敏感等优点，特别是样品保存不理想时更有价值，利用套式PCR可以提高检测的特异性；夹心ELISA检测法具有快速、敏感等优点，但在样品保存不理想时会出现假阴性结果。

She assumptions Road: Emilio segre should have found the first transuranic element neptunium, if he is not a lack of adequate knowledge of chemical and rare earth elements to make mistakes to 23,993 words; Similarly, Enrico Fermi should have been found in fission, if his team The chemical separation technology is not quite so appropriate.

她假设道：Emilio segre本应当发现第一个超铀元素镎，如果他不是缺少足够的化学知识而把23993错认作稀土元素的话；与此相似，Enrico Fermi本应该发现裂变，如果他的研究小组的化学分离技术不是这样不甚适当的话。

New-style analyse palace produces conclusion not to oversew mural layer peritonaeum can make celiac straight flesh and uterus compact adhesion, bring about when the operation enters an abdomen, depart again adhesion time is long, loss of celiac straight flesh is big, adhesion is more traditional the uterus issues paragraph of analyse to produce art serious, to again the operation increased difficulty, what through new-style analyse palace peritonaeum of layer of wall yield art oversews is improved, not only the advantage that retained new-style analyse palace to produce, and the adhesion that block stopped celiac straight flesh and uterine cut, do not disturb especially and injure bladder, do not give again analyse palace is produced bring difficulty, it is a kind of very good new-style analyse palace produces improved art.

结论新式剖宫产不缝合壁层腹膜可使腹直肌和子宫致密粘连，导致再次手术进腹时分离粘连时间长，腹直肌损伤大，粘连较传统子宫下段剖产术严重，给再次手术增加了难度，通过新式剖宫产术壁层腹膜缝合的改良，不但保留了新式剖宫产的优点，而且阻断了腹直肌和子宫切口的粘连，非凡是不扰乱和损伤膀胱，不给再次剖宫产带来困难，是一种很好的新式剖宫产改良术。

Isolates CNR-1 and CNR-2 were from Brassica napus; CNA-1and CRW-1 from Rhododendron simsii and Oxalis corymbosa respectively. All isolates grew well in R-2 liquid medium and exhibited contractive movements. The colonies of all isolates were circinal and grain-like in solid medium. Through electron microscopy, all isolates exhibited helicity during their growth phase. All isolates could pass through a 0.22 μm filtrate membrane and resist to penicillin (2000 U/mL). They must grow in medium with serum.

这4株螺原体在R-2液体培养基中生长良好，都能通过孔径为0.22 mm的微孔滤膜；在R-2固体培养基上呈圆形或颗粒状菌落，菌落直径约50~600 mm；在生长的某个阶段可呈典型的螺旋状，菌体直径为37.04~370.40 nm，长度约0.89~11.88 mm；它们都能利用葡萄糖作为碳源，不能利用尿素；在不含胎牛血清的R-2培养基中，它们都不能生长；菌株CNR-1、CNA-1能强烈代谢精氨酸，而CNR-2和CRW-1不能代谢精氨酸；在氨苄青霉素钠浓度高达2000 U/mL的R-2培养基中，分离菌株生长良好。

Moreover,France virtually controlled Scotland,where the mother of the young Queen ruled as Regent for her daughter,lately married to the Dauphin in Paris,and wh...

席慕蓉 印记不要因为也许会改变，就不肯说那句美丽的誓言，不要因为也许会分离，就不敢求一次倾心的相遇。

The government extension system is the main body of agricultural technology extension system participated by agricultural scientific research department, farmer cooperate organization, supply and marking assn, enterprise and the multitude organization sodality.

然而面临全球经济一体化、加入WTO和农业新技术革命的形势，农业技术推广体系的种种问题逐渐暴露，存在着农业推广机构设置不合理，定位不恰当，运行制度存在缺陷，农业推广资金投入不足，推广与科研、教育分离，基层农技推广人员素质差，观念陈旧等问题，制约着推广力度。

The essence of agent problem is that managers may stray away from the objectives of shareholders and take advantage of the enterprises'resources to seek their own benefits due to the unbalance in terms of information, and risks, the incompleteness in terms of agreement, which all result from the separation of owner -ship and management and due to the fact that shareholders and managers have different interests.

代理问题的本质是所有权和控制权的分离所造成的信息不对称、风险不对称、契约不完全，再加上经营者的利益与股东的利益不一致，使得经营者有可能偏离股东们的目标，利用公司的资源去追求自己的私利。

The day of his and Lydia's departure soon came, and Mrs. Bennet was forced to submit to a separation, which, as her husband by no means entered into her scheme of their all going to Newcastle, was likely to continue at least a twelvemonth.

转眼之间，他和丽迪雅的行期来到了，班纳特太太不得不和他们分离，而且至少要分别一年，因为班纳特先生坚决不赞同她的计划，不肯让全家都搬到纽卡斯去。

And the nature of local government characters the legal rules to deal with local government's debts. There are differents in principle and system design. Three principals, no disincorporate administrative organization, no liquidation government finance, separation between financial bankruptcy and government administrative function, should be followed.

地方政府的特殊性决定了地方政府债务处理的法律规则应与一般破产制度在操作原则和具体制度设计上存在不同，在地方财政破产中应贯彻行政主体不消灭、政府财政不清算、财政破产与政府行政职能分离等原则。

We, then, following the Holy Fathers, all with one consent, teach men to confess the one and same Son, our Lord Jesus Christ, the same perfect in Godhead and also perfect in manhood; truly God and truly man, of a reasonable soul and body; consubstantial with the Father according to the Godhead, and consubstantial to us according to the Manhood; in all things like unto us, without sin; begotten before all ages of the Father of the Father according to the Godhead, and in these latter days, for us and for our salvation, born of the Virgin Mary, the Mother of God, according to the Manhood; one and the same Christ, Son, Lord, Only-begotten, to be acknowledged in two natures, inconfusedly, unchangeably, indivisibly, inseparably; the distinction of natures by no means taken away by the union, but rather the property of each nature being preserved, and occuring in one Person and one Subsistance, not parted or divided into two persons, but one and the same Son, and only begotten, God the Word, the Lord Jesus Christ, as the prophets from the beginning concerning him, and the Lord Jesus Christ himself has taught us, and the Creed of the holy Fathers has handed down to us.

我们跟随圣教父，同心合意教人认识同一位子，我们的主耶稣基督，是神性完全、人性亦完全者；他真是上帝，也真是人，具有理性的灵魂，也具有身体；按神性说，他与父同体；按人性说，他与我们同体；在凡事上与我们一样，只是没有罪；按神性说，在万世之前，为父所生；按人性说，在晚进时日，为求拯救我们，由上帝之母，童女马利亚所生；是同一基督，是子，是主，是独生的，具有二性，不相混乱，不相交换，不能分开，不能离散；二性的区别不因联合而消失；各性的特点反得以保存，会合于一个位格，一个实质之内；而非分离成为两个位格，却是同一位子，独生的，道上帝，主耶稣基督；正如众先知论到他自始所宣讲的，主耶稣基督自己所教训我们的，诸圣教父的信经所传给我们的。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。