不分离的

By extending the concept of MBE technique and employing the uniform buffers to separating the sample electrophoresis, the zone electrophoresis was developed. The isotachoporesis technique is based on the separation in a discontinuity form between two buffer solutions and results in separation of samples. The isoelectric focusing is a method that concentrates the ampholyte sample by the pH gradient introduced in the background ampholytes.

区间电泳法以移动边界电泳法作为基础的技术，其在系统环境充满均匀的缓冲液进行分离；等速电泳法则利用两缓冲液进行不连续分离之技术；等电点聚焦电泳法则利用背景溶液建立出pH值梯度，双性电解液样品因其位於等电点所受库伦力为零而聚焦於此。

T. , the isolated yield reached to 92% and the selectivity was above 99%. The product was immiscible with TSILs and could be separated simply by decantation after the reaction.

由于生成的乙酸苄酯不溶于催化体系，反应产物与催化体系分层，通过简单的倾析便可实现产物分离，简化了分离过程。

The second variation type is called reversion evolution type, which represents the liquid segregation mainly with vapour-liquid fractional distillation. The third variation type is called saltatory variation type, which is formed from the liquid segregation mainly with immiscibility.

称为正向演化类型，是结晶分异结果；②称为反向演化类型，代表气液分馏为主的液态分离；③称为演化突变类型，是不混溶为主的液态分离形成。

AIM: To isolate bioactive secondary metabolites from the fruits of Schisandra rebriflora Rehd. et. Wils and study their effects on HIV-1 infectivity. METHODS: Inhibition of syncytia formation, HIV-1 reverse transcriptase and protease, protection of HIV-1 infected cells, level of HIV-1 p24 antigen,blockage of fusion were detected. RESULTS: A lignan compound, named rubrifloralignan A, was first isolated as a natural product with modest inhibition effects on syncytium formation and HIV- 1 replication and could protect HIV-1 infected cells with weak inhibition effects on fusion and RT, but it showed no effects on the replication of HIV-1 chronic H9 and PR. CONCLUSION: Rubrifloralignan A is an efficient anti-HIV-1 product with effect on early stage of HIV-1 replication.

目的：从红花五味子果实中寻找活性代谢产物，研究其抗HIV—1活性和作用机制方法：通过合胞体抑制、HIV-1感染细胞保护、HIV—1p24抗原测定、融合阻断、逆转录酶和蛋白酶活性分析等实验，检测分离化合物的抗HIV—1活性并探讨其作用机制一结果：红花五味子甲素能够抑制病毒诱导的合胞体形成，保护病毒感染细胞，抑制病毒在细胞内的复制对HIV-1感染细胞与正常CD4^＋细胞间的融合，逆转录酶活性有一定的抑制作用但是红花五味子甲素不抑制病毒在慢性感染细胞中的复制，也不抑制蛋白酶的活性、结论：首次从天然产物中分离得到红花五味子甲素，它对HIV—1病毒复制早期具有抑制作用

In case the Apostles had celebrated the Lord's Supper during the triduum mortis (the time during which Christ's Body was in the tomb), when a real separation took place between the constitutive elements of Christ, there would have been really present in the Sacred Host only, the bloodless, inanimate Body of Christ as it lay in tomb, and in the Chalice only the Blood separated from His Body and absorbed by the earth as it was shed, both the Body and the Blood, however, hypostatically united to His Divinity, while His Soul, which sojourned in Limbo, would have remained entirely excluded from the Eucharistic presence.

在案件使徒们庆祝了主的晚餐期间triduum mortis （时间，在基督的尸体是在墓穴中），当一个真正的分离之间发生构成要件的基督，本来就不会有真正目前，在神圣的主办单位只，不流血，无生命基督的身体，因为它卧在墓穴中，并在chalice只有血液分离，从他的身体和吸纳由地球，因为这是大棚，无论是身体和血液，但是， hypostatically团结，以他的神性，而他的灵魂，其中sojourned在莲步，一直处于完全排除在圣体圣事的存在。

The A and B components remanent magnetization were loaded by goethite and maghemite respectively, the C component remanent magnetization was carried by the magnetite in the sandstones and were loaded conjunctly by the magnetite and hematite in the mudstones and muddy sandstones.

它们的存在并不影响岩石原生剩磁的分离，也不影响原生剩磁信号的稳定性和获得的古地磁数据的可靠性。16个砂岩采点中不能分离出C分量，是由于后生的赤铁矿彻底改变了由磁铁矿携带的原生剩磁组分，在高密度采样的情况下剔除这些采点并不影响古地磁极性柱的构建和解释。

In case the Apostles had celebrated the Lord's Supper during the triduum mortis (the time during which Christ's Body was in the tomb), when a real separation took place between the constitutive elements of Christ, there would have been really present in the Sacred Host only, the bloodless, inanimate Body of Christ as it lay in tomb, and in the Chalice only the Blood separated from His Body and absorbed by the earth as it was shed, both the Body and the Blood, however, hypostatically united to His Divinity, while His Soul, which sojourned in Limbo, would have remained entirely excluded from the Eucharistic presence.

在案件使徒们庆祝了主的晚餐期间triduum mortis （时间，在基督的尸体是在墓穴中），当一个真正的分离之间发生构成要件的基督，本来就不会有真正目前，在神圣的主办单位只，不流血，无生命基督的身体，因为它卧在墓穴中，并在chalice只有血液分离，从他的身体和吸纳由地球，因为这是大棚，无论是身体和血液，但是， hypostatically团结，以他的神性，而他的灵魂，其中sojourned在莲步，一直处於完全排除在圣体圣事的存在。

We initiated a genetic screen for Drosophila olfactory genes by means of SG18.1-Gal4/UAS system: The result validates our screen as an rapid, effective approach for recovering genes controlling glomerular map patterning; From a misexpression screen of 1,515 P{GS} lines, we identified 23 genes that, when forcibly expressed in the olfactory receptor neurons, disrupted the stereotyped anatomy of the Drosophila antennal lobes; These genes, which have not been shown previously to control olfactory map development, encode novel proteins as well as proteins with known roles in axonal outgrowth and cytoskeletal remodeling.

本文首次利用SG18.1-Gal4／UAS基因筛选系统对果蝇嗅觉基因进行了筛选和鉴定：结果表明，所采用的方法可以快速、有效地分离到果蝇嗅觉相关基因；从1515株果蝇P{GS}品系分离到86株突变体，其中40株有明显表型，从中鉴定了23个基因，这些基因在ORN强迫表达时可以损坏果蝇嗅叶的固有结构；在筛选中发现的一些调节嗅觉图谱发育的基因可以编码新蛋白或编码参与轴突生长和细胞骨架重塑的蛋白；为了便于研究，根据基因的功能和所编码蛋白的特点，我们对分离到的基因进行了分类，即参与轴突引导和突触产生、调节转录、调节细胞骨架和功能未确定或未知的基因；并通过对嗅觉感觉器的检测发现，除了调节转录的基因外，其他基因对触觉器的数量和分布影响不明显。

This project installs cyclone separator behind the carbueter of vaporization section in residue-type chemical fertilizer ammonia plant and revamps the carbueter structure so that the ash flows with cooling water to the separator instead of remaining in the carbueter.

渣油型化肥厂合成氨气化工段的气化炉后加置旋液分离器，改造气化炉结构，使灰渣不滞留于气化炉中，随激冷水流到旋液分离器中，将激冷水中全部能堵塞后序流程设备的灰渣沉淀，定期排放到屯渣槽中，干净的不含有堵塞设备的灰渣的碳黑水送往碳黑回收工段。

The isolated cells are able to orient and migrate to the magnet in artificial magnetic field but could not orient swimming only under the geomagnetic field. Magnetosomes produced by Acidithiobacillus ferrooxidans were range from 40nm to 90 nm according to the results of TEM. Energy dispersive X-ray analysis indicated that extracted magnetic particles consisted of oxygen and iron. The results show that some Acidithiobacillus ferrooxidans cells have weak magnetotaxis and they could be able to be separated by solid-plate magnetophoresis method from others. With the development of this new isolation method, it is possible that we could do deeper research to generate a comprehensive description of the mechanism that how Acidithiobacillus ferrooxidans synthesize the magnetic particles.

实验结果表明，氧化亚铁硫杆菌具有较弱的趋磁性，在人工磁场下会进行趋磁运动，但仅在地磁场作用下不能定向运动，利用固体平板磁泳筛选纯化含有磁性颗粒的氧化亚铁硫杆菌的方法是切实可行的，磁泳分离技术的进一步完善和改进为传统的微生物菌种分离提供了新的途径，为研究纯氧化亚铁硫杆菌菌株胞内磁性颗粒的形成条件及机理提供了前提条件，也为今后从浸矿细菌中分离筛选更多的含有磁性颗粒的菌株打下基础。

The concept of equivalent rotationally rigidity is offered and the formula of rotationally rigidity is obtained.

主要做了如下几个方面的工作：对伸臂位于顶部的单层框架—筒体模型进行分析，提出了等效转动约束的概念和转动约束刚度的表达式。

Male cats normally do not need aftercare with the exception of the night after the anesthetic.

男猫通常不需要善后除了晚上的麻醉。