不分离的

The uncovered tracts and the liver wound surface were managed by some of the methods combined such as high frequency electrocoagulation, titanium clamp, suture, hemostatic gauze oppression, argon-beam coagulation, protein glue adherence, and Endo-GIA switcher. The total operative outcome, operating time, blood loss, postoperative complications and postoperative hospital stay were summarized in order to assess feasibility and safety of the clinical laparoscopic liver resection.Results: 1. Laparoscopic liver resection with clamp dissection method needs only the ordinary laparoscopic equipment. It has a low cost but causes a little more bleeding. 2. Laparoscopic liver resection operated by microwave tissue coagulation method had very little bleeding when dissecting hepatic parenchyma. The operative field is clear and the cost is low. It is a safe, convenient and effective method. 3. Ultrasound dissector is a relatively ideal appliance for laparoscopic liver resection at present because ofless bleeding during operation. But the speed is slow thus prolongs the operation time. Its separating intensity is not strong enough, and it can only be applied to patients without hepatic cirrhosis. 4. Ligsure has clamp dissection and electrocoagulation functions. It can carbonize hepatic tissue including the tracts whose diameters are less than 7mm. It has some advantage in dissecting. However, it has the shortcomings of the clamp dissector during coagulating. 5. Hand-assisted laparoscopic liver resection can make use of the flexibility and feeling of the operators left hand.

结果：1、钳夹分离切肝法不需特殊仪器设备，只要具备常规的腹腔镜器械即可实施，成本低，肝断面较易渗血；2、微波固化切肝法肝实质出血很少，视野清晰，成本低，操作简单，是一种安全、简便、有效的方法；3、超声刀是目前较为理想的切肝器械，术中出血少，但是，切割速度慢，手术时间长，而且，切割强度有限，适用于无肝硬化的肝切除；4、Ligsure具有挤压粉碎及高频电凝止血两大功能，可使包括7~以下管道的肝组织碳化、形成焦痴，具有一定的优势，但是，也存在分离钳电凝止血的缺点；5、手辅助腹腔镜肝切除可以利用术者左手的灵活性及手感，便于术中显露、分离、控制出血等，可随意协助右手及助手的主要操作，缩短了手术时间，大大提高了腹腔镜肝切除的安全性，李朝龙等的改良方法，同样达到了手辅助目的，又节省了费用；6、小切口腹腔镜辅助切肝法可以使用剖腹肝切除常规器械及剖腹肝切除技术，操作简单、可靠，适用于肝左外叶切除及右肝第V段切除；7、Endo一GIA切肝法在离断肝组织的同时闭合管道结构，多用于肝左静脉、门静脉分支、管径较大胆管的切割，缺点是不能用于较厚肝组织的切割，而且价格昂贵；8、临床巧例腹腔镜肝切除均获得成功，手术时间最短1.5h，最长sh，平均125 min，多数在100 min左右，手术出血量最少50 ml，最多500 ml，平均1 78 ml，除2例合并严重肝硬化的原发性肝癌患者术后出现少量腹水，1例术后发生胆漏外，其余无并发症，发生胆漏的1例患者术后住院40d，多数在术后一周左右出院，术后住院时间5一40d，平均gd。

Thress homologous chromosomes of trisomics associated as a trivalent or a bivalent and a univalent or three univalent during diakinesis and metaphase 1 ;Laggard chromosome and univalent separated in advance at anaphase I ,leading to distribution of 10-1-10,9-12,11-11,10-2-10,10-12,10-10,9-1-11 besides 10-ll;And there was distribution of 11-11-10-10, 10-10-10-12, 10-10-10-11, 10-11-11-11, 11-11-10-9, 10-10-10-10, 9-12-10-10, 9-9-11-11, 11-10-10-9, 12-10-9-11, 11-9-9-13, 10-10-10-9 at anaphase II ;n l microspore frequency were different of four trisomics.

三体在终变期和中期Ⅰ，3个同源染色体联会成三价体，或2个联会成二价体和1个单价体，或3个都为单价体；后期Ⅰ出现落后染色体或单价体提前分离，导致除10-11式分离外，还有10-1-10，9-12，11-11，10-2-10，10-12，10-10，9-1-11等不均衡分离方式；后期Ⅱ有11-11-10-10，10-10-10-12，10-10-10-11，10-11-11-11，11-11-10-9，10-10-10-10，9-12-10-10，9-9-11-11，11-10-10-9，12-10-9-11，11-9-9-13，10-10-10-9等分离方式；4个三体中的n 1小孢子频率不同。

There are some isoforms of PAPs showing inhibitory ability to virus infection in various organs and at different stages of development. Three different species of the protein can be isolated from leaves of pokeweed. Proteins were precipitated with 50-80% ammonium sulfate from leaves in spring, summer and autumn respectively. Proteins were purified on SP cation-exchange then on MQ anion-exchange and finally on MS cation-exchange or on MQ anion-exchange. We found that some new pokeweed antiviral proteins separated from these leaves were different from PAP, PAPII and PAPIII.

对美洲商陆春季、夏季、秋季采集的叶片，用50-80％饱和度硫酸铵沉淀出来的蛋白样品，经过SP阳离子柱后，上MQ阴离子柱，然后把活性蛋白峰再上MS阳离子柱或MQ阴离子柱分离，从春季叶片中分离出四个不同的抗病毒蛋白峰；从夏季叶片中分离出二个不同的抗病毒蛋白峰，这两个蛋白峰与春季叶片中分离出的活性蛋白峰都能重叠上；但从秋季叶片中只分离出来一个抗病毒蛋白峰，它和春季、夏季叶片中分离出的活性蛋白峰都重叠不上。

There are some isoforms of PAPs showing inhibitory ability to virus infection in various organs and at different stages of development. Three different species of the protein can be isolated from leaves of pokeweed (PAP, PAPⅡ and PAPⅢ). Proteins were precipitated with 50-80% ammonium sulfate from leaves in spring, summer and autumn respectively. Proteins were purified on SP cation-exchange then on MQ anion-exchange and finally on MS cation-exchange or on MQ anion-exchange. We found that some new pokeweed antiviral proteins separated from these leaves were different from PAP, PAPⅡ and PAPⅢ.

对美洲商陆春季、夏季、秋季采集的叶片，用50-80％饱和度硫酸铵沉淀出来的蛋白样品，经过SP阳离子柱后，上MQ阴离子柱，然后把活性蛋白峰再上MS阳离子柱或MQ阴离子柱分离，从春季叶片中分离出四个不同的抗病毒蛋白峰；从夏季叶片中分离出二个不同的抗病毒蛋白峰，这两个蛋白峰与春季叶片中分离出的活性蛋白峰都能重叠上；但从秋季叶片中只分离出来一个抗病毒蛋白峰，它和春季、夏季叶片中分离出的活性蛋白峰都重叠不上。

A virus strain was isolated from the arthrosis of broiler chickens.The virus was shown to have spherical particles with isometric symmetry,no envelope,double deck capsids,with outer capsid of 75nm,inner one of 50nm in diameter by electron microscopy.The virus was not sensitive to aether,acid and heat.The virus could multiply on the chick embryo fibroblastcell,and could cause syncytial pathologic change with the appearance of cytoplasm inclusion bodies.The challenged chickens of one-day old by nasal,oral,intramuscular and pulvillus injection displayed obvious clinical symptom of severe arthritis and accompanied with pathological changes of malabsorption and necrosis.Meanwhile,four avian reovirus isolates CL,S1133,YH and YB were examined in the cross-neutralization test.

从临床患病鸡关节病料中分离到一株病毒，在电镜下观察到病毒粒子无囊膜，有双层衣壳，外衣壳直径约75nm，内核约50nm；分离病毒株对酸、热有较强的抵抗力，对乙醚不敏感；病毒感染鸡胚成纤维细胞上能产生以融合细胞为特征的CPE；爪垫接种1日龄雏鸡表现出明显的病毒性关节炎临床症状，并伴有吸收障碍型和坏死型的病理变化；血清中和交叉试验表明，该病毒分离株与国内的2株番鸭源呼肠孤病毒分离株和国外S1133呈不同程度的交叉反应。

Single spore strains without producing fruit body were isolated from the original Cordyceps militeris, WWM04 with producing fruit body.

对优势蛹虫草原始菌株WWM04进行单孢子分离，共获得9个单孢子分离株，采用米饭培养基培养确证并且获得6个完全不产子实体的单孢子分离株。

In conclusions: 1 The treatment that buffalo occytes are cultured in Ionomycin (5 min)+CDM (3 h)+ 6-DMAP(3 h) after ICSI with sorted sperm can improve their subsequent embryonic development; 2 The dead ultrasoniced sorted sperm can be used for ICSI; 3 The result of ICSI with sorted sperm is similar to that with unsexed sperm, but the result of IVF with sorted sperm is lower than that with unsorted sperm; 4 The treatment of heparin after ICSI with sorted sperm can improve the blastocyst development rate; 5 The development rate of blastocyst with sorted sperm was variable among buffalos indicating that sorting high quality sperm from specific buffalos can increasethe efficiency of ICSI.

结果表明：1水牛分离精子ICSI后用Ionomycin(5min)+CDM(3h)+6-DMAP(3h)方法激活处理能提高其胚胎发育率；2分离后的死精子经超声断尾也能用于水牛卵母细胞的ICSI；3分离精子的ICSI效果不低于未分离精子，但分离精子的IVF效果却低于未分离精子；4肝素能促进分离精子ICSI后的胚胎发育；5分离精子ICSI后的胚胎发育率在不同公牛之间有差异，选择能产生高质量精子的公牛进行精子分离，可以提高ICSI的效率。

The cells were cultured under the following serum microenvironment. The primary cells of autoserum group were cultured with autoserum, changing the medium with fetal bovine serum after passage. The primary cells of homogeneity foreign serum group were cultured with homogeneity foreign serum, changing the medium with fetal bovine serum after passage. The primary cells of fetal bovine serum group were cultured with fetal bovine serum, and cultured with fetal bovine serum after passage. The primary cells of Dulbecco's modified Eagle's medium group were cultured with serum-free DMEM, changing the medium with fetal bovine serum after passage.

分别以下列血清微环境培养：自身血清组：分离细胞后原代用含大鼠自身血清的培养基培养，传代后换用含胎牛血清的培养基培养；同种异体血清组：分离细胞后原代用含同种异体血清的培养基培养，以后用胎牛血清培养基培养；胎牛血清组：分离后用含胎牛血清培养基培养，以后一直用含胎牛血清培养基培养；DMEM组：分离细胞后原代用不含血清的DMEM培养基培养，传代后换用含胎牛血清的培养基培养。

The silicone rubber membrane used in SRMBR is a polymer-made non-porous material. It is hydrophobe and non-permeable to inorganic salt, and has the high selective ethanol permeability. Compared to the conditional separation technology, the pervaporation through SRM has the advantages of low-cost and low-pollution, especially applicable to separating volatile matter from organic matter-water system or organic-organic matter system.

其中的硅橡胶膜作为一种均相聚合物材料无孔膜，具有不透无机盐成分、疏水和对乙醇有较高选择透过性等特点；与传统分离技术相比，硅橡胶膜的渗透蒸发分离过程具有低能耗、污染少等优点，尤其适用于在有机物-水或有机物-有机物体系中分离挥发性有机物组分，现已用于发酵下游产物分离处理的试验研究中，并取得初步成效。

For the hyper-critical,"to boldly go where no man has gone before" should be "to go boldly…" It is good to be aware that inserting one or more words between "to" and a verb is not strictly speaking an error, and is often more expressive and graceful than moving the intervening words elsewhere; but so many people are offended by split infinitives that it is probably better to avoid them except when the alternatives sound strained and awkward.

对那些吹毛求疵的人来说，&to boldly go where no man has gone before&应为：&to go boldly…&。在介词&to&和动词之间插入一个或多个单词，严格说并没有什么错，而且往往比随意插入更优美、表达更充分，但很多人对分离不定式大为不满，认为除非更改会造成牵强附会和蹩脚，否则避免使用分离不定式可能会更好。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。