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不分性别的

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First of all, love is the subject regardless of age and gender.

首先,爱的主体是不分年龄和性别的。

After amplying a 2.2kb fragment form the PPV-SC1 RF-DNA,we clone the fragment into pMD 18-T,named pTNSl.The whole sequence which is 1989 bp long was determined by sequencing, including the complete ORF of PPV-SC1 NS1 which encoding 662 amino acids.Alignment of pairs of sequence indicates that there are 98% and 99% similary with other porcine parvovirus strains Kresse and NADL-2, respectively. Multiple sequence alignment discloses that there are a few difference between ppv-scl nsl gene and other ppv nsl gene: A-G at 39nt,T-C at 153nt,A-G at 175nt, A-C at 1117nt, A-C at 1535nt .Alternative codon in ppv-scl nsl have distinctly different frequentfy by codonbias analysis at EMBOSS(http://genopole.toulouse.inra.fr/bioinfo/emboss). Thereis not distinct hydrophobicity and transmenbrane helices in ppv-scl nsl protein. Struction domain anslysis of PPV-SC1 NS1 protein indicate that there are a ATP/GTP-binding site motif A at 398-405,16 Protein kinase C phosphorylation site,21 Casein kinase II phosphorylation site,and 3 cAMP/cGMP-dependent protein kinase phosphorylation site.At the same time ,there is a same motif between ppv-scl nsl and Poxvirus D5 protein-like which may share in the same fuction which is necessary during virion duplication.

将PPV-SC1 NS1序列与其他PPV NS1基因进行多序列比对,结果显示,PPV-SC1 NS1与其他的PPV NS1的同源性较高,仅存在个别的差异,分别是第39位A→G,第153位T→C,第175位A→G,第1117位A→C,第1535位A→C;同源搜索比较表明,PPV-SC1与PPV NS1同源性可达98%、99%,与其他的细小病毒NS1基因也存在很大的保守性;密码子偏向性分析结果表明PPV-SC1 NS1基因在同一氨基酸的不同密码子的选择上存在一定的偏向性;PPV-SC1 NS1蛋白总体上说具有亲水性不存在明显的疏水性区段,用swiss TMPRED软件预测PPV-SC1 NS1的跨膜区,返回的结果并没有得到有显著意义的跨膜区的存在;根据基于motif数据库的结构域预测,PPV-SC1 NS1的第393-415位氨基酸残基存在潜在的ATP/GTP结合位点,该蛋白还存在16个蛋白激酶C磷酸化位点,21个酪蛋白激酶2磷酸化位点,3个cAMP-/cGMP依赖蛋白激酶磷酸化位点,PPV-SC1 NS1蛋白与POX_D5(痘病毒D5蛋白)具有一致的保守结构域,推测NS1可能与POX_D5有类似的功能。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。