不分化的

The traits of dedifferentiation and redifferentiation of the explants form H. sampsonii show that the calli the stem and leaf usually form buds at its parietal cells. In the presence of auxin, the parenchyma of the leaf gap at the node is initiated firstly, then, develops blastemata that form buds in a cluster on the node without callus. These show that the various parts ofthe herb differ greatly in cell initiation and organ differentiation.

研究元宝草外植体的脱分化和再分化的特点结果表明，茎段和叶片启动形成愈伤组织，其不定芽通常发生在愈伤组织的周边区，茎节叶腋的叶隙薄壁组织在激素的诱导下启动、分裂，不形成愈伤组织而直接器官分化成芽原基，并在节结处产生一圈成簇的不定芽，表明元宝草不同部位的细胞启动及器官分化调控存在差异。

The results above showed:(1) The cultivars of large-flowered chrysanthemum are widely distributed in Beijing area with plentiful floral types and floral colors;(2) The cultivars with the rare types and colors are urgent for protection;(3) Most of the quantitative characters obeys to the normal distribution,some important traits agreed with skewed distribution.The probability grading can assist quantitative character in establishing new grading standards;(4) Most of qualitative characters is asymmetrical in cultivars,which might be caused by unbalanced differentiation of characters or linkages coming from emphasis characters selection;(5) The floral characters are relatively stable and have comparably large influence on classification,the characters of stipule also have a certain degree of stability and can act as an reasonable supplement for cultivars classification,but the foliar characters have worse stability and can only be recognized as lower level criterion.(6) The genetic relationship among the Flat-petaled group,the Quilled-petal group and the Spoon-petaled group are closest,which followed by the Filiform-petal group because of long-term selection pressure for the specialization of the petal tip loved by most people.The Anemone-petal group has further genetic relationship with these four groups.(7) ISSR molecular marker detection indicates that the proportion of polymorphic loci of lardge-flowered chrysanthemum is higher,in which the Flat-petaled group has the highest genetic diversity level.

综合以上的研究结果表明：(1)北京地区现有大量大菊品种，且花型与花色较为丰富；(2)珍稀花型、花色品种亟需保护；(3)大部分数量性状是符合正态分布的，少数重点观赏性状迫于选择压力呈现偏态分布，概率分级法能够辅助大菊的数量性状建立新的分级标准；(4)大部分质量性状在品种群内的分布是不均匀的，可能是由菊花性状遗传分化的不均衡性或受到选育重点性状的连锁作用而造成的；(5)大菊花部性状相对稳定，对品种分类影响较大，其托叶部分也具有一定程度的稳定性，可以作为品种分类的合理补充，而叶部性状稳定性较差，在品种分类中应作为更次一级的分类依据；(6)平瓣类、管瓣类和匙瓣类的亲缘关系最近，畸瓣类品种花瓣先端特化受到人们的喜爱，长期的选择压力使其区别于3种基本瓣型，而这四种瓣型与桂瓣类在亲缘关系上较远；(7)ISSR分子标记检测表明：大菊品种多态性位点百分率较高，平瓣类品种的遗传分化最为丰富。

Results: The differentiated neuron appeared different patterns of amitotic figures such as transverse sever, longitudinal split, etc. The proliferating neurons divided in an asymmetric way morphologically and chemically. The differentiated neuron appeared an obvious movement of cell migration and neurite extension.

结果：培养分化的神经细胞呈现横裂、纵裂等不同方式的无丝分裂像；分裂中的神经细胞可呈现形态及化学不对称性；分化的神经细胞有明显的细胞迁徙运动和突起延伸。

The short shoot differentiation came from the lateral buds among the bud scales, in the initial stage, the lateral buds elongating, and then differentiating two "anisomerous"(one was small, the other was big) needle primordia in the elongating courses; the big one differentiating once more, and formed three needle primordia.

短枝的发育来源于长枝上芽鳞间的侧芽，在发育的初期，侧芽进行伸长生长，在伸长生长的过程中首先分化出一大一小&不对称&的两个针叶原基，大的叶原基进一步分化，形成三个针叶原基。

Autologous MSC can be obtained easily,their source in human are plenty , own active proliferationand potential to difference into chondroblast.

自体骨髓基质干细胞来源丰富，取材容易，具有旺盛的增殖能力及向成软骨细胞分化的潜能，已成为软骨组织工程的首选种子细胞，但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善，目前主要采用的技术为细胞团块离心管培养，其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Autologous MSC can be obtained easily, their source in human are plenty, own active proliferation and potential to difference into chondroblast. Now MSC become the chief seed-cell in cartilage tissue engineer, but the technology to stimulate MSC differentiate into chondroblast is not very successful, the standard technology is culture the cell-ball in centrifuge tube, the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

自体骨髓基质干细胞来源丰富，取材容易，具有旺盛的增殖能力及向成软骨细胞分化的潜能，已成为软骨组织工程的首选种子细胞，但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善，目前主要采用的技术为细胞团块离心管培养，其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Autologous MSC can be obtained easily,their source in human are plenty , own active proliferationand potential to difference into chondroblast. Now MSC become the chief seed-cell in cartilage tissue engineer , but the technology to stimulate MSC differentiate into chondroblast is not very successful,,the standard technology is culture the cell-ball in centrifuge tube,the reconstructured cartilage-like tissue is not achieve the mechanics of hyaline cartilage.

自体骨髓基质干细胞来源丰富，取材容易，具有旺盛的增殖能力及向成软骨细胞分化的潜能，已成为软骨组织工程的首选种子细胞，但MSC诱导分化为成软骨细胞且应用于软骨组织工程的技术尚不十分完善，目前主要采用的技术为细胞团块离心管培养，其构建的软骨组织在力学性能上很难达到关节软骨的要求。

Vitrification, chlorosis, scale-shaped differentiation and failure to root of regenerated shoots frequently occur in industrialized production of tetraploid watermelon Citrullus vulgaris L.

西瓜四倍体工厂化生产中存在试管苗玻璃化、黄化死亡、鳞片状分化、不生根等不利问题，在MS中NH4NO3 含量减半的情况下，前两者可得到有效的控制；用连续增殖、连续壮苗的方法，解决了西瓜四倍体鳞片状分化的难题；用茎尖扩繁的最佳培养基为MS(含半量NH4NO3 )+2 。

In well-differentiated tumors, HSP27 expression was higher than that in normal epithelia.

在低分化的肿瘤细胞中完全不表达，而在高分化的肿瘤细胞染色很深。

The expression pattern of Triae;CYCD2;1 was analyzed by RNA blot hybridization and in situ hybridization , the results revealed that Triae;CYCD2;1 expression is associated with the proliferation tissues, particularly in meristems and leaf primordia.

组织切片结果显示35S：：Triae；CYCD2；1转基因拟南芥叶片的栅栏组织和海绵组织分化不明显，多数细胞排列已不规则，茎表皮下多层细胞明显变短，苗端分生组织明显增大且具有较多未分化的小细胞。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。