不凋的

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling . Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

The following methods were used in the study, such as viral inoculation of animal, pathological method, flow cytometry, RT-PCR, in situ hybridization, immunohistochemistry and terminal deoxy nucleotidyl transferase mediated dUTP nick end labling. Morphological changes of the infected chickling, dynamic changes of T, B cells and T subsets, changes of adhesion molecules on the lymphocyte surface, necrosis and apoptosis of lymphocyte and neurons, changes of MIP-1β and IL-8 producing cells in the brain, cell types of perivascular tuffing in cerebral tissue were systematically studied. The key research results were.(1)The average percentage of CD19+cell in blood, bursa and spleen, after 3, 5 days of inoculation, was significantly Phybridization revealed that the number of IL-8 and MIP-1βproducing cells was increased in the infected brain.

在研究过程中，采用了病毒接种技术、普通病理学研究方法、流式细胞仪技术、RT-PCR技术、原位杂交技术、免疫组化技术、凋亡细胞末端标记技术，系统研究了不同日龄的SPF雏鸡人工感染AEV-NH937株后的病理变化，T和B淋巴细胞、T淋巴细胞亚群的动态变化规律，淋巴细胞表面某些粘附分子的变化，淋巴细胞和神经元的坏死与凋亡，雏鸡脑组织中产生趋化因子MIP-1β和IL-8细胞的变化规律，脑组织中围官性细胞浸润的细胞类型。

In conclusion, the lag phase of Streptococcus thermophilus was shorten in the eutrophy culture medium, and the time achieving the culminated point of logarithmic phase was shorted also, at the same time, the stationary phase lengthened. After secondary culture, Streptococcus thermophilus reached directly the log phase hardly through the lag phase, and then quickly reached the decline phase. There were more relations of the metabolic regalities of the three kinds of nutritive substances with the super-concentrated incubation process of Streptococcus thermophilus: the more the number of the bacteria, the quicker the metaboling, while the fewer, the slower the metabolism. Moreover, there apparently existed morphologic changes in this course, and there maybe existed the apoptosis, which correlated with bacteria propagation and acid producing. With the culture time postponed, the nutritive substances in the culture medium lacked also, and the morphologic change and apoptosis appeared more obviously.

结果表明，嗜热链球菌在营养丰富的培养基中的生长延滞期缩短，到达对数生长顶点的时间变短，稳定期的时间延长，当经过二次培养后，嗜热链球菌几乎不经过延滞期直接到达对数期，平稳期持续的时间较短，很快到达衰亡期；嗜热链球菌在超浓缩培养过程中三大营养物质的代谢规律也同它的培养过程有很大的关系，菌数多，其代谢旺盛，菌数降低其代谢速度减慢；嗜热链球菌在超浓缩培养过程中存在着明显的菌体形态变化，并可能存在着细胞凋亡，与菌体增殖和产酸规律密切相关，随着培养时间延长，培养基中营养物质变得贫乏，菌体形态变化和细胞凋亡的情况愈加明显。

Methods The mouse thymocyte model of apoptosis was induced by dexamethasone. The apoptosis ratio was observed with flow cytometry technique.

方法采取体外细胞培养 Balb/c小鼠(体质量 20 g左右的 Balb/c小鼠，雌雄不限)胸腺细胞的方法，制备地塞米松诱导的细胞凋亡模型，通过流式细胞仪观察凋亡细胞数量的变化情况。

Pentylenetetrazol is a central nervous system drug that induce chronic and acute epilepsy model to study epileptogenous mechanism .Kindling is a natural epileptogenous phenomenon.

我们前期研究工作发现：应用电镜、Timm染色等从形态结构方面已证实在点燃前神经元发生了可塑性改变包括苔藓纤维芽生，新突触形成(突触密度增大或数量增多)与突触重排等，但这些只是一个初步探索，仍不能够说明问题的全部，如其分子生物学基础、神经元凋亡情况等尚不清楚，需进一步研究探讨。

Results Nicotinamide at 1-5 mg/ml induced apoptosis of GLC-82 cells in a dose-dependent manner, and group 4 mg/ml and group 5 mg/ml showed increased apoptosis than other groups. Distinct morphologic changes of cell apoptosis such as karyopyknosis and conglomeration were observed; Cell migration became slower as the dosage of Nicotinamide increased;Cells produced an arrested at G1 phase, accompanied by a reduction of cells transiting through S phase.

结果 烟酰胺诱导细胞凋亡程度与用药剂量呈正相关，较高剂量的药物能引起GLC-82肺腺癌细胞呈现边界不清，体积缩小，核固缩，染色质凝集、边缘化，甚至细胞发生破碎，凋亡小体形成；随着烟酰胺浓度的增高GLC-82细胞向划痕区的愈合速度不断减慢；烟酰胺的浓度大于等于3 mg/ml 时，细胞周期主要表现为G1 期比例的上升伴随S期比例的下降。

We may well explain why ricin-induced apoptosis showed no typical DNA ladder. The presence of laddering in DNA gels implies an apoptosis process, but its absence does not rule out the apoptosis. The ricin 0.05μmol〓 had no effect on the 〓 phase of cell cycle, but induced 〓 arrest. The ricin may affect the balance of synthesis and destruction of proteins that controlling the cell cycle.

检测到DNA梯度表明细胞发生了细胞凋亡，未检测到DNA梯度不能否认凋亡的存在。0.05μmol.L〓的蓖麻毒素对HeLa细胞周期的G〓/G〓期无影响，而诱导了G〓/M期阻滞，蓖麻毒素可能引起一些控制细胞周期蛋白的合成与降解发生紊乱。

The Food and Drug Administration of USA hasapproved the use of this topical medicine for treatment of different malignant andpremalignant tumors such as actinic keratoses and basal cell carcinoma. Althoughimiquimods exact mechanisms of action in neoplastic diseases has not yet been fullyelucidated, animal and human studies have shown its ability to enhance the productionof cytokines such as interferon-, interleukin-12, and tumor necrosis factor- though conjugations with Toll like receptors on the surface of dendriticcells of the monocyte-macrophage lineage.

有关咪喹莫特抗癌作用的具体机制仍不十分明了，一般认为，咪喹莫特主要是通过与免疫相关细胞表面的Toll样受体结合、促进IFN-α、TNF-α、IL-12等多种前炎症细胞因子的分泌，进而激活天然与获得性免疫反应的方式发挥抗癌作用；进一步的研究发现，咪喹莫特尚能选择性的抑制某些皮肤肿瘤细胞的增殖并诱导其凋亡，提示增殖抑制和诱导凋亡可能是评价咪喹莫特抗肿瘤作用的重要指标和机制。

Berbamine could inhibit the growth of leukemia cell line NB4. The induction of cell apoptosis may be one of the mechanisms for suppressing the growth of leukemia cell line NB4. Inhibition of Survivin mRNA and upregulation of Caspase 3 protein might be also involved in cell apoptosis.

小檗胺对NB4细胞有增殖抑制作用，诱导细胞凋亡可能是其抗肿瘤作用的重要机制之一，但不是通过作用于NB4细胞特异性融合基因PML/RARα发挥作用的，其抗凋亡的可能分子机制之一是抑制Survivin基因的表达和增加Caspase3的表达。

This thesis compared the effect of Uracil, ITS, and ATP on the maturation of bovine oocytes and further development, observed the microstructure changes of oocytes and parthenogenetic embryos among different groups, and granule cell apoptosis among different groups.

本文对比了添加尿嘧啶和三价因子等试剂对牛卵母细胞成熟及后期发育的影响、观察了不同组之间成熟牛卵母细胞和孤雌激活胚胎显微结构变化和不同组之间颗粒细胞凋亡情况。

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称；三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起，称之为挽歌体诗人或者史诗诗人，他们被称为诗人，似乎只是因为遵守格律写作，而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。