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The sizes of the self-assembly micelles of the amphiphilic block copolymers and the effects werestudied by dynamic light scattering and UV-visible spectrophotometer. The micelle sizes werelargely influenced bythe hydrophobic chain content in the copolymer, the properties of the solvent and the copolymer concentration in the organic phase. The morphology of the micelles was investigated by transmission electron microscopy. Theresults showed that the micelles appeared spheres with inner core and outer shell. The critical association concentrations were determined by pyrene monomer fluorescence probe technology. The degradability of copolymer was studied by the loss of the intrinsic viscosity in the degradation process. The results indicated that the degradation rate of PECL was slower thanthat of PELLA and PEDLLA. The stability of the copolymer micelle dispersion was examined by measuring the critical flocculation concentration, which gradually decreased with increasing the content of thehydrophobic chain in the copolymer. The rheological results showed thatthe viscosity ofthe micelle dispersion with higher content of PEG segments was firstly decreased and then increased with the increasingthe temperature. Otherwise, the variation of viscosity was irregular. The viscosity of the micelle dispersion increased with the increase of the electrolyte (Na 2SO 4) concentrations. Polymer micelles paclitaxel was prepared by self-emulsification/solvent evaporation method and solid dispersion technique with amphiphilic block polymers as the carrier material.

运用动态光散射和紫外分光光度计研究了两亲性嵌段共聚物自组装胶束的粒径及其影响因素，结果表明，自组装胶束的形成机理受制备方式的控制，胶束的粒径随着共聚物相对分子质量或疏水嵌段相对分子质量和有机相中共聚物浓度的增大而增大，随着有机溶剂的水溶性的增强而减小；采用透射电镜观察胶束的形态结构，发现胶束呈具有核壳结构的均匀球形；采用芘荧光探针法测两亲性嵌段共聚物的临界聚集浓度；通过降解过程中共聚物特性粘度的变化研究两亲性共聚物的降解性能，发现与PELLA和PEDLLA的相比，PECL 降解速率较慢；随着共聚物中疏水嵌段含量的增大，纳米分散液的稳定性逐渐下降；PEG含量高的PEDLLA纳米分散液随着温度的升高，纳米分散液的粘度先下降然后增大，反之，没有规律性，且随着电解质Na 2SO 4浓度的增大，体系粘度增大。

Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

Results 1.After treated by:program freezing,the maximum load of the allografts has no evident decrease and the cell activity of allografts was preserved well,the frozen hurt after program freezing was more slight than －80℃ deepfrozen,2.No evident reject reaction can be seen during the healing process after deep frozen and the reject reaction declined as the time pass by.3.Three weeks afer the allografting,there was no significant difference between the two al lografting groups in maximum load(P＞0.05),Eight weeks later,the maximum load of the group that was preserved by liquid nitrogen after program freezing(76.60±4.67)N was better than the group that was preserved by －80℃ deepfrozen(75.99±4.20) and similar with the autografting group(76.72±2.55).4.The healing process and histological behavior of the two deepfrozen groups are similar with the autografting group,and the program freezing group is better.

结果 a经程序冷冻液氮保存方法处理后，髌腱的最大载荷无明显下降，细胞活性得到了较好的保存，组织学观察冷冻损伤较－80℃深低温保存方法轻微；b程序冷冻液氮保存处理的移植物在术后未表现明显的排斥反应，且免疫反应随时间的推移而下降；c移植后3周，各组移植物的最大载荷无显著差异(P＞0.05)，移植后8周，程序冷冻液氮保存组移植物的最大载荷(55.87±1.86)N优于－80℃深低温保存组(52.14±2.79)，而和自体移植组相近(57.70±2.76)N；d从组织学观察看，－80℃深低温保存组和程序冷冻液氮保存组移植后的愈合过程均和自体移植组相似，而程序冷冻液氮保存组的愈合过程和组织学行为更接近于自体移植组。

Finally, the pH value, lactic acid, water soluble carbohydrate, ammonia nitrogen and cellulose contents were determined for WH silage prepared under the suitable adding doses of lactobacillus and cellulase at day 1, 3, 5, 15, 25, 35 after ensilaging. The pH value decreased from 5.5 before ensiling to 4.3 on the 35th ensiling day, whereas the reverse change tendence was observed for lactic acid content. The cellulose content of silage was lower from 26.5%DM in raw material to 22.3%DM.

结果表明：pH值由青贮前的5.5至青贮第35天时降至稳定的状态，而乳酸含量的变化趋势相反；凤眼莲茎叶纤维素含量从未青贮前的26.5%干物质，至青贮第35天降至22.3%干物质，降低了约4个百分点；青贮料中可溶性碳水化合物的含量随青贮时间的延长而逐渐下降，但是在第5天到第15天的时间段里，可溶性碳水化合物的含量无显著性下降；氨态氮占总氮的比例从青贮第1天的4.61%显著上升至第5天的8.25%，而从第5天开始到第35天，氨态氮占总氮的比例上升的幅度较小。

In traditional agricultural production , farmers always fertilizes the land at their own will , which not only increased the cost of agriculture production and worsened the soil fertility , but also caused serious environmental pollution and threaten .

传统农业生产中，由于农民盲目施肥现象严重，这样不仅造成肥料利用率下降，也增加了农业生产的成本，使得耕地地力下降，而且也带来严重的环境污染，威胁农产品质量安全。

The results showed that steroid potentiates hypercoagulable and hypofibrinolytic states which would play an important role in inducing and developing osteonecrosis, these might cause IC, forming intraosseous thrombi, which would further cause ischemia of bone marrow and bone, thus result in osteonecrosis.

这表明激素助长了高凝和纤溶下降状态，高凝和纤溶下降在激素性骨坏死发生发展中起重要作用，二者异常引起骨内IC，形成骨内血栓，引起骨缺血乃至骨坏死。

The sprouts from endplate and a few contact sites of NCAM with AChR can be seen.C: Untreated group on the 20th day after L5 nerve root compression. There are some AChR plaques of morphological abnormality, and no contact sites of NCAM with AChR although the intensity and scope of NCAM staining is increased.D: Yiqi Huayu Recipetreated group on the 20th day after L5 nerve root compression. All the AChR sites are reoccupied by NCAM and the morphology of AChR are restored. E: Untreated group on the 30th day after lumbar nerve root compression. AChR plaques display irregularity in shape, with a few round or spindle shaped cupulae. Meanwhile, some NCAM stainings are still shown to be around the AChR. F: Yiqi Huayu Recipetreated group on the 30th day after L5 nerve root compression.

随着时间的推移，AChR斑的形态渐趋规则，NCAM反应也逐步增强，至造模后20 d达到最高峰，但益气化瘀方组仍然明显优于模型组，其不仅在数值上超过了胚胎期大鼠，且与AChR全部重叠；而模型组仍可见到不少重叠不全且形态异常的AChR斑。L5神经根受压后30 d，NCAM的表达水平开始下降，基本局限于终板区内，其下降速度和范围在益气化瘀方组明显大于模型组；模型组不仅NCAM的表达明显高于益气化瘀方组，且仍能见到一些成熟不全的AChR斑；而益气化瘀方组AChR斑形态已接近于正常。

There are some AChR plaques of morphological abnormality, and no contact sites of N-CAM with AChR although the intensity and scope of N-CAM staining is increased.D: Yiqi Huayu Recipe-treated group on the 20th day after L5 nerve root compression. All the AChR sites are reoccupied by N-CAM and the morphology of AChR are restored. E: Untreated group on the 30th day after lumbar nerve root compression. AChR plaques display irregularity in shape, with a few round or spindle shaped cupulae. Meanwhile, some N-CAM stainings are still shown to be around the AChR. F: Yiqi Huayu Recipe-treated group on the 30th day after L5 nerve root compression.

随着时间的推移，AChR斑的形态渐趋规则，N-CAM反应也逐步增强，至造模后20 d达到最高峰，但益气化瘀方组仍然明显优于模型组，其不仅在数值上超过了胚胎期大鼠，且与AChR全部重叠；而模型组仍可见到不少重叠不全且形态异常的AChR斑。L5神经根受压后30 d，N-CAM的表达水平开始下降，基本局限于终板区内，其下降速度和范围在益气化瘀方组明显大于模型组；模型组不仅N-CAM的表达明显高于益气化瘀方组，且仍能见到一些成熟不全的AChR斑；而益气化瘀方组AChR斑形态已接近于正常。

There are some AChR plaques of morphological abnormality, and no contact sites of NCAM with AChR although the intensity and scope of NCAM staining is increased.D: Yiqi Huayu Recipetreated group on the 20th day after L5 nerve root compression. All the AChR sites are reoccupied by NCAM and the morphology of AChR are restored. E: Untreated group on the 30th day after lumbar nerve root compression. AChR plaques display irregularity in shape, with a few round or spindle shaped cupulae. Meanwhile, some NCAM stainings are still shown to be around the AChR. F: Yiqi Huayu Recipetreated group on the 30th day after L5 nerve root compression.

随着时间的推移，AChR斑的形态渐趋规则，NCAM反应也逐步增强，至造模后20 d达到最高峰，但益气化瘀方组仍然明显优于模型组，其不仅在数值上超过了胚胎期大鼠，且与AChR全部重叠；而模型组仍可见到不少重叠不全且形态异常的AChR斑。L5神经根受压后30 d，NCAM的表达水平开始下降，基本局限于终板区内，其下降速度和范围在益气化瘀方组明显大于模型组；模型组不仅NCAM的表达明显高于益气化瘀方组，且仍能见到一些成熟不全的AChR斑；而益气化瘀方组AChR斑形态已接近于正常。

Chain rental home price survey, in 2004 the Olympic region apartment rental business in 2003 than a decline, with an average reduction of 500 yuan / month, in which European classical 3-bedroom rental in 2003 by the 6000 yuan / month, down 2004 5,500 yuan / month, while the Sunshine Plaza 3-bedroom rent in 2004 than in 2003 reduced by 1,000 yuan / month.

连锁出租房屋价格调查显示，在2004年奥林匹克区域公寓租赁业务在2003年比下降，平均下降500元/月，在欧洲古典3居室租金在2003年的6000元/月，低于2004年5500元/月，而阳光广场3居室的租金在2004年比2003年减少了1000元/月。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。