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An area of (1.5×1.5) cm2 autologous full thickness skin graft model was made on the back of the all the rats. The color change of skin graft was observed at different time points. Meanwhile, whether hematocele and fluidify as well as binding to wound appeared under the skin graft were investigated.

所有大鼠背部制作(1.5×1.5)cm2面积的自体全层皮肤移植模型,在不同时间点观察移植皮片的颜色改变,移植皮片下是否有积血、积液,皮片是否与创面粘合。

By in situ hybridization, 53BP2 mRNA expression is revealed in tumor cell nests of NPC paraffin sections. The strong positive particles localize in the nuclei of tumor cells; but slight positive signals are also found in tissue cells of chronic nasopharyngitis and connective tissue, such as lymphocytes and smooth muscle cells; There are also positive signals in the nuclei of hyperplastic squamous epithelial cells. However, 53BP1 mRNA expression is not found in nuclei of tumor cells, tissues of chronic nasopharyngitis and connective tissue.

原位杂交结果显示,石蜡切片的鼻咽癌癌巢内,可见53BP2的强阳性表达信号,信号呈紫色颗粒状分布于胞核,核仁清晰可见;而在相同条件下,在慢性鼻咽炎组织和癌旁上皮的结缔组织,也可见弱表达信号,如淋巴细胞和平滑肌细胞;在增生的鳞状上皮细胞的核内,可找到强阳性信号。53BP1在鼻咽癌、慢性鼻咽炎组织和癌旁上皮的结缔组织切片上,均无表达信号。

On a ZORBAX Stable Bound (2.150 mm, 1.8 m), the rutin、quercitrin、quercitrin-3-O-giucoside and quercitrin-3-O-galactose could be separated completely with 0.05 mol/L potassium dihydrogen phosphate and methanol as mobile phase (40:60,V/V) at a flow rate of 0.3 ml/min and detected with a photodiode array detector .

在色谱柱为ZORBAX Stable Bound (2.150 mm, 1.8 m),流动相为0.05 mol/L的磷酸二氢钾缓冲溶液-甲醇(40:60,V/V),流速为0.3 ml/min,在检测波长为360 nm的色谱条件下,芦丁、槲皮素、槲皮素-3-O-葡萄糖苷和槲皮素-3-O-半乳糖苷分离效果好,方法灵敏度高,重现性好,用于测定雪莲果叶中的上述几种黄酮芦丁型黄酮,效果满意。

Changes in brain tissue similar to those of newborn in HIE were detected after 2 hours of hypoxia and ischemia.Meanwhile,swelling of kidneys,hemorrhage below encapsules,swellin of tubular epithelial cells,necrosing and falling of a few tubular epithelial cells,and narrowing of tubular lumens were observed.The basement membranes of renal tubules remained normal ...

结果发现新生猪经2小时缺氧缺血后,在脑组织出现类似新生儿HIE病理损害的同时,肾脏出现肿胀,包膜下出血,肾小管上皮细胞肿胀,管腔变窄,少数上皮细胞坏死脱落,肾小管基膜完整,血清尿素氮及肌酐明显升高(P<0.025及P<0.05),表明在HIE状态下新生猪已出现肾脏的病理及功能损害,但损害程度较轻,解除缺氧72小时后,上述损害基本恢复正常。

In rat esophagus, the positive varicosities and fibres could be observed in the circular muscle layer and the muscularis mucosae layer at 21st day before birth. With the development of rat digestive tract, neurokinin A-immunoreactivity positive nerves could be observed gradually in the epithelium, submucosa, longitudinal muscle layer, myenteric plexus and submucosal plexes, while mature nerve fibres could be seen at one month after birth. 2. In rat stomach, the positive reaction of NKA initially happened in the myenteric plexus at the 14th day of embryo, and then appeared on circular muscles, longitudinal muscles, submucosal, muscularis mucosae, lamina propria and epithelium. 30 days after birth, expression of NKA is same as seen in adaulthood. 3. In rat small intestinal, the NKA-IR could first be found in the myenteric plexus of the duodenum, jejunum and ileum at 14th , 15th and 17th day before birth respectively. Then the NKA-IR occurred in the longitudinal muscle layer, circular muscle layer, intestinal villus, intestinal gland, muscularis mucosae, submucosa, submucosal plexes, mucosa plexus and deep muscular plexus.

结果 1、在食管,于胚胎21天的粘膜肌层和环肌层内观察到神经激肽A免疫阳性膨体纤维,出生后,随幼鼠的生长发育,相继在上皮,粘膜下层,纵肌层、肌间丛、粘膜下丛有NKA-IR表达,30天时已和成年鼠相似;2、在胃,首先于胚胎14天的肌间丛出现NKA-IR的表达,随发育相继在环肌、纵肌,粘膜下层、粘膜肌、固有膜及上皮内出现NKA-IR的表达,30天时具备成年鼠的分布特征;3、在小肠,分别于胚胎14、15、17天的十二指肠、空肠和回肠的肌间丛处出现NKA-IR的表达,随发育相继出现在纵肌、环肌、绒毛、小肠腺周、粘膜肌、粘膜下层、粘膜下丛、粘膜丛、深肌丛。

By contrasted with silica gel, the corn hull desiccants by hydrolyzation method and heat treatment method have advantages as stabilization on moisture adsorption, less water of reversible adsorption and high moisture adsorption ratio in high humidity. These desiccants have disadvantages as lower moisture ratio than silica gel in low humidity and slower moisture adsorption speed than silica gel. The corn hull desiccant by derivation method has advantages as high moisture adsorption ratio, high moisture adsorption speed, stabilization on moisture adsorption and hardly any water of reversible adsorption.

通过与变色硅胶干燥剂的对比,通过水解法和热处理法得到的玉米渣皮干燥剂的优点是吸水稳定,可逆吸附水少,在高湿度下吸水率高,不足是在较低的湿度下,吸水率比变色硅胶干燥剂略低,吸水速度比硅胶慢;通过衍生方法得到的玉米渣皮干燥剂的优点是吸水率高,吸水速度快,吸湿稳定持久,几乎不含可逆吸附水,不足是再生后产品容易结块。

Abstract] Objective To investigate the change of bronchial mucosa cilia epithelium structure on children with recurrent respiratory tract infection in acute stage and convalescence stage.Methods Choose 38 cases of patients with RRTI as study group,respiratory ciliated cells were collected by bronchial fiberscope in acute stage and convalescence stage respectively,observe the change of cilia structure by electron microscopy and compare the incidence of cilia cylindrical epithelium structure abnormality.

摘要] 目的研究反复呼吸道感染患儿急性期与恢复期支气管黏膜纤毛上皮结构的特点与变化,临床治疗的有效性提供客观依据方法选择符合儿童RRTI诊断标准的患儿38例作为研究对象,分别于急性期及恢复期在纤维支气管镜下夹取支气管黏膜组织,电镜下观察纤毛上皮结构的特点并比较纤毛异常的发病率,探讨RRTI患儿治疗后纤毛的恢复情况。

Sometimes PP mouse may be in bad mood also, at this moment she always keep silence only without saying one word , like a scared little mouse, nobody can get her out of the depression but the lawyer, lawyer is always get his special way to lead his customer out of dead end, once PP mouse in bad mood, the lawyer will carry her in his arm and frusta her hip slightly, one.two..three… until she beg for mercy with laughing..

皮皮鼠也有不高兴的时候,有时莫名其妙的生气,也许是老鼠性在作怪,生气时一声不吭,倒真有点象老鼠了,对老公也不理不睬,可律师总归是律师,总能想出好办法逗她,看到她的小鼠脸拉长了,便抱起她在桌子上墩她的屁股墩,一下、二下、三下……。。她不得不大叫求饶,笑作一团…。

Basing on the setup of animal model of root avulsion of the brachial plexus, we divided the experimental animal into four groups by the operative method: Group A: one end of the maternal nerve graft was connected with the upper trunk of the healthy side by end-to-side neurorrhaphy, and the other end was sutured to the distal stump of musculocutaneous nerve infraclevicularly by end-to-end neurorrhaphy. Goup B: on the healthy side, the operation was the same, the other end was sutured to the distal end of C〓 by end-to-end neurorrhaphy on the affected side. Group C: the maternal nerve graft bridged the phrenic nerve and infraclevicular musculocutaneous nerve of the affected side by end-to-end neurorrhaphy. Group D: the phrenic nerve was sutured to the distal end of C〓 supraclevicularly by end-to-end neurorrhaphy.

在构建了模拟小儿臂丛神经根性撕脱伤的动物模型基础上,将实验动物按手术方式分组如下,A组:母鼠提供的的神经移植体一端与子鼠健侧臂丛上干行端侧吻合,另一端与患侧已切断的锁骨下肌皮神经远端行端端吻合。B组:母鼠提供的的神经移植体一端与子鼠健侧臂丛上干行端侧吻合,另一端与患侧已切断的锁骨上颈〓远端行端端吻合。C组:母鼠提供的的神经移植体桥接于子鼠患侧膈神经与锁骨下肌皮神经之间。D组:子鼠患侧膈神经直接与锁骨上颈〓远端行端端吻合。

In order to produce chitin derivative with high-quality, the processing for producing chitin from puparia of silkworm Bombyx mori were optimized: puparia were dipped in 3% HCl for 22 h at 30℃, then in 5% NaOH for 4 h at 85℃, followed by decoloring in 10% H2O2 for 0.5 h under 85℃.

为制备高品质甲壳素衍生物,研究了利用家蚕蛹皮制备甲壳素的最佳工艺条件:将家蚕蛹皮用3% HCl在30℃条件下浸泡22h,再用5%NaOH在85℃条件下水浴4h,然后用10%H2O2于85℃下加热脱色0.5h。

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