下游

Gonorrhoeae was amplified with the one set of primer for the 16S rRNA gene. The reverse primers were labeled with biotin. The products were identified by hybridization with its specific oligonucleotide probes labeled with amidogen in a nylon membrane.

选择NG 16S rRNA基因设计一对PCR引物，生物素标记下游引物扩增NG DNA，然后与固定在尼龙膜上的特异性寡核苷核探针杂交。

Herodotus calls the Dai a Persian nomad tribe, but they can not have lived in Persia proper, because they are mentioned in the Anabasis of Arrian as living along the lower reaches of the Syrdar'ya.

希罗多德呼吁傣族 1波斯游牧部族，但他们不能一直住在波斯适当的，因为他们中提到假的安作为居住在下游

Design another set of specific primers, of which upstream and downstream primers were located in the two sides of the cleavage site respectively, to develop the one-step RT-PCR method for detecting H5 and H9 subtypes of AIV. Identify the sensitivity of the developed one-step RT-PCR method by detecting 2 AIV strains of H5 subtype and 3 AIV strains of H9 subtype, and the specificity by detecting the RNAs of NDV, IBV and IBDV as well as analogue detection using computer software.

根据H5、H9亚型HA基因序列，分别设计一套特异性引物，其上、下游引物分别位於裂解位点两侧，应用RT-PCR一步法检测AIV H5和H9亚型，并验证所建立方法的敏感性；通过检测NDV、IBV和IBDV等RNA病毒及电脑软件模拟检测，验证其特异性。

The Upper Tunguska is the lower course of the Angara River.

上通古斯卡河是安加拉河的下游。

Northern Lanna Thai has also expanded its forces, and in 1296 its capital city of Chiang Mai,.14 century, the Chao Phraya River downstream emerged a powerful Ayutthaya Dynasty (1350-1767), annexed Sukhothai, torn of Angkor Thom.

北部的兰那泰王国也扩大了势力，1296年定都清迈城。14世纪中，湄南河下游出现了强大的阿瑜陀耶王朝（1350—1767），吞并了素可泰，蹂躏了吴哥城。

And the more "upstream," culture-friendly, purpose driven, and wise perspectives should not be disregarded in favour of "downstream", more immediate anthropocentric risks and consequences.

而较"上游"的和谐文化、驱动力和明智的观点，也不能不顾"下游"的利益，更为现时的人类风险和后果。

In those years, anticyclone circulation prevails in Northern China with ridge to the western side of Beijing.

北京及附近地区位于低压槽区或处在东高西低的下游"阻塞"形势，是北京夏季多雨的基本环流条件。

Along the Mekong River, flooding is becoming increasingly worse for the downstream countries in recent years while aridness is also getting worse during dry seasons.

沟通在湄公河流域，这几年下游国家的洪灾越来越严重，但旱季时却变得更加干旱，那里的人们感到很气愤，认为是中国在上游不负责任地修大坝造成的结果。

The differential expression profiles of 588 known genes were determined using Atlas human cDNA expression array. Fifteen genes were differentially expressed following the overexpression of BNIPL-1. Most of them were involved in cell proliferation or cell apoptosis. Furthermore, the differential expression result was confirmed by semiquantitative RT-PCR.

然后采用人Atlas cDNA expression array对新基因BNIPL-1在细胞内的下游调控事件进行了初步研究。588个已知基因中有15个的表达发生了改变，其中大部分是细胞生长和凋亡相关基因，半定量RT-PCR验证了杂交结果的可靠性。

The Silk Road gave rise to the clusters of military states of nomadic origins in North China, invited the Nestorian, Manichaean, Buddhist, and later Islamic religions into Central Asia and China, created the influential Khazar Federation and at the end of its glory, brought about the largest continental empire ever: the Mongol Empire, with its political centers strung along the Silk Road (Beijing in North China, Karakorum in central Mongolia, Sarmakhand in Transoxiana, Tabriz in Northern Iran, Astrakhan in lower Volga, Bahcesaray in Crimea, Kazan in Central Russia, Erzurum in eastern Anatolia), realizing the political unification of zones previously loosely and intermittently connected by material and cultural goods.

丝绸之路引发了由中国北部开始的游牧地区军队丛生，引发了景教、摩尼教、佛教，后期还有伊斯兰宗教进入中亚和中国，创造了有影响力的哈扎尔联盟。在它的显赫终结之时，带来了曾经是最大陆地面积的帝国：蒙古帝国，它的政治中心是沿着丝绸之路（中国北部的北京，蒙古中部的哈拉和林、河中地区的萨马卡罕、北伊朗的大不里士、伏尔加河下游的阿斯特拉罕、克里米亚的巴塞莎拉、中俄罗斯的喀山、安纳托利亚东部的埃尔祖鲁姆），意识到先前松散和被物质和文化商品间歇地连接的地区需要政治上的统一。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失