下游

The coding sequence of grouper Epinephelus coioides growth hormone gene was cloned under the regulation of carp β-actin gene promoter and enhancer, so an"all-fish"recombinant pCAecGHc was constructed, then the construct was microinjected into eggs of Lepomis cyanellus and the transgenic Lepomis cyanellus was produced.

通过基因重组，将石斑鱼生长激素基因编码序列克隆到鲤鱼β-actin基因启动子下游，构建了&全鱼&生长激素基因表达载体pCAecGHc。

Signal peptide sequence was removed lest it couldnt be recognized by Bacillus subtilis, gene coding for mature peptide was ligated to downstream of sacB signal peptide sequence of pWB980 to form a new ORF and generate pWB980-LipA, gene coding for its chaperone was also amplified and ligated to the downstream of LipA to generate a secretion/expression vector pWB980-LipAB.thechaperone gene was sequenced and analyzed by multiple alignments, resulted showed that there were mutations of nucleotides(1～7) and amino acids (1～2) with the other reported genes.

为了防止枯草芽孢杆菌信号肽酶不能识别脂肪酶的信号肽，切除掉脂肪酶的信号肽编码序列，与枯草芽孢杆菌分泌表达质粒pWB980连接并与质粒上的SacB信号肽构成一个完整的开放式阅读框，获得重组质粒pWB980-LipA，因为该脂肪酶的活性表达需要一个特异性分子伴侣帮助折叠成有活性的构象，将脂肪酶分子伴侣基因串连到脂肪酶基因的下游，获得重组分泌表达载体pWB980-LipAB并且测序分析分子伴侣的基因序列和氨基酸序列并与其它报导的序列进行了比对，发现有1到7个碱基的差异和1到2个氨基酸残基的突变。

High-fat feeding of both strains led to decreased serum triglycerides, hepatic steatosis, and hypercholesterolemia; howeer, B6 mice deeloped worse steatosis and a larger increase in LDL cholesterol. Both B6 background and high-fat feeding increased sterol regulatory element-binding protein-1c (SREBP-1c), a key regulator of lipogenic gene transcription, and its downstream targets.

两种品系的高脂喂养都导致血清甘油三酯降低，脂肪肝，和高胆固醇血症；然而，B6小鼠产生更严重的脂肪肝和低密度脂蛋白胆固醇的增加。B6遗传背景和高脂喂养都增加甾醇调解元件结合蛋白-1c（SREBP-1c），它是脂肪生成基因转录和它的下游靶点的关键调解元件。

Mr Mandil said: Everybody from producing to consuming countries has to ensure that there is enough investment in the energy chain, from upstream to downstream.

芒迪先生说，&从产油国到石油消费国的每一方，都必须确保能源链从上游至下游拥有足够的投资。&

The muscle PCBs concentration of Tilapia and Mugil cephalus from downstream Tan-Shui River were 3.852 and 6.582 ppb.

分析淡水河下游吴郭鱼及乌鱼肌肉含多氯联苯的浓度分别是3.852与6.582 ppb。

This part is to explore the effect of rhEPO on neuromotor function,secondary pathology change and expression of Caspase-3, which was peritoneal injected at different time after rat traumatic spinal cord injury Method Wistar rat were randomized into normal group,sham operation group, blank control group and rhEPO group.

第二部分脊髓损伤后促红细胞生成素对Caspase-3表达的影响目的：半胱氨酸蛋白酶被认为是细胞凋亡过程中最重要的一系列蛋白酶，其中Caspase-3是最重要的蛋白酶，是多种凋亡途径的共同下游效应部分。

Bromine and its mainly engaged in the production of downstream products and research and development, bromine annual output of 4,000 tons of products, quality standards in line with GB 2021-94, 2,6 dibromo 4 nitroaniline, more than 1,000 tons of annual production, quality to meet Customers as a standard, best-selling products at home and abroad.

主要从事溴素及其下游产品的生产和研发工作，溴素产品年产量4000余吨，质量符合GB 2021-94标准，2，6二溴4硝基苯胺，年产量1000余吨，质量以满足客户为标准，产品畅销国内外。

PKC plays an important role in nonasthmatic ASMCs proliferation, which is induced by many mitogens. However, to date, in the ASMCs proliferation, the signal transduction effect and downstream of PKC haven't been well studied.

但目前国内外尚少见对PKC在ASMC增殖调控中的信号转导作用及其活化后的下游途径进行深入研究。

The results show that the Reynolds stresses of the flow near the wall at the exit are abviously nonisotropic and the flow fluctuation transport downstream with the flow speed.

结果表明埋入式进气道出口近壁区气流的雷诺应力呈明显的各向异性，其上游的气流脉动是按气流平均速度向下游传输的。

Based on parabolized stability equations , the stability of nonparallel boundary layer is calculated and analyzed by using recursion numerical method.

该文从抛物化稳定性方程出发，采用从上游往下游递推的数值方法，对非平行边界层稳定性问题进行了数值计算和分析。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。