下游

Up to May 2000, before water delivering, the cutting off runoff of lower Tarim River had been continued for 30 years, all the surface water had disappeared for a long time. It forces the environment which is originally controlled by dry circulation to develop rapidly toward dry environment. The desertification had expanded greatly. As the results, a large number of the saline meadow and halophilous shrub deserta had disappeared.

：由于近30a的断流，使本来就处于干旱环境中的塔里木河下游末端朝着更加干旱的方向急剧发展，致使原有的荒漠植被大量消失，植物成片死亡，或濒于死亡，并形成大片裸地；遗留种类极度贫乏，群落结构简单，一年生草本植物趋于灭绝。

In arid climates, surface water is generally better than ground water in headwater areas, but poorer downstream.

在干旱气候的上游区，地表水一般较地下水好，但下游的地表较差。

Random amplified polymorphic DNA technique was employed to detect the DNA polymorphism in 20 individuals of Coreius heterodon in the lower reach of the Yangtze River. 19 arbitrary primers were used and 90 repeatable RAPD bands were generated.

用随机扩增多态DNA技术对长江下游铜鱼20个个体的遗传多样性进行了检测，从40个随机引物中筛选出19个对每个铜鱼的DNA进行扩增。

Comparing the genetic diversity of Coreius heterodon with that of other fishes, the experiment revealed that the genetic diversity of Coreius heterodon in the lower reach of the Yangtze River was a little lower.

通过与其他鱼类的遗传多样性的研究结果比较可初步判断，长江下游铜鱼群体的遗传多样性较低。

Random amplified polymorphic DNAtechnique was employed to detect the DNA polymorphism in 20 individuals of Coreius heterodon in the lower reach of the Yangtze River. 19 arbitrary primers were used and 90 repeatable RAPD bands were generated.

用随机扩增多态DNA技术对长江下游铜鱼20个个体的遗传多样性进行了检测，从4O个随机引物中筛选出19个对每个铜鱼的DNA进行扩增。

Executive plan May:professional consultation,inquire on the net,data collection,training These data includes:the names, photos,habits,the condiction of distribution and the level of severe danger about the aquatic hydrophilous wild animal in the lower reaches of the DaDu River and the drainage area of the MaBian River.

实施方案五月：专家咨询，网上查询，资料收集，人员培训这些资料包括，大渡河下游及马边河流域野生珍稀水生动物的名称，图片，生活习性，分布现状和濒危级别。

The silicone rubber membrane used in SRMBR is a polymer-made non-porous material. It is hydrophobe and non-permeable to inorganic salt, and has the high selective ethanol permeability. Compared to the conditional separation technology, the pervaporation through SRM has the advantages of low-cost and low-pollution, especially applicable to separating volatile matter from organic matter-water system or organic-organic matter system.

其中的硅橡胶膜作为一种均相聚合物材料无孔膜，具有不透无机盐成分、疏水和对乙醇有较高选择透过性等特点；与传统分离技术相比，硅橡胶膜的渗透蒸发分离过程具有低能耗、污染少等优点，尤其适用于在有机物-水或有机物-有机物体系中分离挥发性有机物组分，现已用于发酵下游产物分离处理的试验研究中，并取得初步成效。

The N gene ORF was then subcloned into pET-30a vector and the recombinant plasmid was transformed into E.coli BL21 (DE3) and induced with IPTG. The protein expression was determined by SDS-PAGE. The expressed protein had a molecular weight of 54.4 Ku that existed as inclusion body. Thin-layer scanning showed that the expression product accounted for 30.5% of the total bacterial proteins. The recombinant protein possessed native biological activity and could react with anti-PEDV hyperimmune serum in Western blot.

以阳性质粒为模板，用分别含有BamH Ⅰ和Xho Ⅰ酶切位点的上、下游引物扩增得到ORF，其PCR产物经BamH Ⅰ和Xho Ⅰ双酶切后定向克隆到pET-30a载体，构建的重组质粒命名为pET-30a-PN；将pET-30a-PN转化到大肠杆菌BL21 (DF3)中，在IPTG诱导下进行表达；SDS-PAGE结果表明表达出与预期大小相符的约54.4 Ku的重组蛋白，重组蛋白以包涵体形式存在；薄层扫描结果表明表达产物占菌体总蛋白的30.5%；Western blot分析表明表达的重组蛋白能与抗PEDV高免血清反应，说明该重组蛋白具有免疫学活性。

Objective To express SZ39 PE40 in the form of GST fusion protein and prepare a promising way for further investigation of the recombinant immunotoxin against glioma.

目的 以GST融合蛋白的形式表达SZ39 PE40 ，为抗胶质瘤重组免疫毒素的下游研究与开发准备一条可供选择的途径。

In ancient times,it is vital to think highly of the irrigative construction and renovation that plays an important part in agricultural economy development,especially for the lower reaches of the Yangtze characteristic of rice-growing.

对农田水利的重视和兴修，是我国古代农业经济发展最为重要的条件之一，对于以植稻为主的长江下游地区来说，农田水利的兴修尤为重要。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。