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In cotyledon, on one hand, very weak GUS staining was observed in PARF8:GUS, but very strong was found in phyA-209 PARF8:GUS. In the shoot apical meristem, hypocotyle and root tip, on the other hand, phyA-209 PARF8:GUS exhibited weaker GUS activity than PARF8:GUS. Interestedly the same expression patterns of ARF8 resulting from disruption of PhyA have been observed in dark, white-light and far-red-light conditions.

结果表明,无论在黑暗、白光和远红光条件下,PhyA突变均明显提高幼苗子叶和叶片中ARF8的表达水平,但明显降低幼苗下胚轴、茎尖和根尖中的表达水平。

In 95% confidence level, HS and GARCH (1,1)-HE are best. In 99% confidence level, PARCH (1,1)-HE is best and HS is also excellent.

在95%的置信水平下,HS和GARCH(1,1)-HE模型为最好;在99%的置信水平下,PARCH(1,1)-HE模型最好,而HS模型同样优秀。

SDS-PAGE results showed that as to Mut+ recombinant highest yield was obtained after 4 days inducing and with the culturetime prolonged it reduced. Pokeweed antiviral protein gene expressed well when methanol concentration reached 10g/L. Pokeweed antiviral protein obtained high yield in thin acidic culture medium (pH6.0-6.4) and its quantity in total mass of secrete protein exceeded 30%.

SDS-PAGE分析结果表明,Mut~+组菌株在甲醇诱导第四天后PAP在培养液中积累量达到最高水平,延长培养时间会导致产量下降;在10g/L的甲醇浓度诱导下,PAP的表达量达到最高;培养基pH值在偏酸性条件下(6.0-6.4)PAP的表达量都维持在较高的水平。

Results: Caspase-3 mRNA was constitutively expressed at a low level in freshlyisolated CD34+ cells. The expression of caspase-3 mRNA and protein was upregulated when these cellswere first expanded in suspension culture with growth factors for 3 days. However, only the 32 kDa inactivecaspase-3 proenzyme was detected in the freshly isolated CD34+ cells as well as during the first 3 daysexpansion with cytokines.

检测结果显示,Caspase-3mRNA在新鲜分离的脐血CD34+细胞中低水平表达,在细胞因子支持下体外培养3天,扩增的CD34+细胞中Caspase-3mRNA和蛋白质水平表达上调,但在这两种细胞中仅能检测到分子量为32KDa的非活性酶原形式的Caspase-3;随着体外培养时间的延长,在IL-3,IL-6和GM-CSF组合条件下,Caspase-3被激活,可检测到分子量为20KDa的裂解片段。

We collected the urinary samples of one adult male, three pregnant females and one oestrus female.

我们收集了一只雄性黑叶猴不同阶段、三只雌性黑叶猴妊娠期和一只雌性黑叶猴发情期的尿液,用放射免疫分析法分别测定了雄猴尿中的睾酮、雌猴尿中的雌二醇和孕酮浓度,分析了不同阶段、不同状态下各种性腺激素水平的变化,并将其与黑叶猴的繁殖行为相联系,探讨笼养状态下黑叶猴性腺激素水平与繁殖行为的关系。

AR model in linear filter theory is adopted to simulate pulsant wind. Combined with some typical cable-membrane structures, research on self-vibration of the structure has been conducted. Based on the previous work, the influence caused by the vertical wind component on the membrane roofs has been analyzed in time domain. It includes the vibration laws of the structure under separate horizontal wind component, separate vertical wind component and the collective horizontal wind component and vertical wind component. Significant conclusions have been drawn from the analysis.

然后本文采用线性滤波器法中的AR模型对风速场进行了模拟;之后又结合典型的张拉索膜结构研究了膜结构的自振特性;在此基础上,本文研究了索膜结构在竖向脉动风影响下的风振响应特性,其中分别研究了张拉索膜结构在单独水平风,单独竖向风以及水平风和竖向风共同作用下的振动规律,得出了有关重要结论。

The pseudo-static test which subjects to horizontal load on the A-type autoclaved aerated concrete block wall was adopted. Reinforced concrete beams were set up at the upper and lower ends of the test block, and the four-bar linkage device was provided to ensure that the top of the wall was only in translatory motion, not in rotation at the level force. Hydraulic synchronous loading device was adopted to simulate the vertical load which was produced by the wall of the housing interlayer.

对A类蒸压加气混凝土砌块墙体采用水平荷载作用下的伪静力试验,试件的上下两端设置了钢筋混凝土梁,通过四连杆装置保证墙体顶部在水平力作用下只有平动而无转动,并采用同步液压加载装置模拟房屋层间墙体作用的竖向荷载。

However, higher concentration of CRP may increase the expression of protein and mRNA of inflammatory factor in endothelial cells; 2 Higher concentration of CRP may participate in the development of inflammatory process in endothelium by the mechanism that CRP induce cells apoptosis and activate signal pathway involved in cell proliferation. 3 CRP is the member of pentraxins family, in which two faces of pentamer form a special bi-phase structure: one face is ligand recognition phase; the other face is effector phase.

总结: 1CRP是一种保守蛋白,在正常生理水平下对内皮细胞没有明显的诱导炎症发生的作用,仅在高浓度条件下有可能诱导细胞炎症相关蛋白及mRNA的表达; 2高于生理水平的CRP对内皮细胞的致炎作用主要通过诱导细胞凋亡及激活细胞增殖相关蛋白信号通路的表达; 3CRP是一种双面五聚体蛋白,结构具有特殊的二相性,能够分别结合内皮细胞和E-LDL。

Three steps, the establishment of distribution modes, equilibrium analysis and simulation comparing, are displayed in the paper and at last the conclusion is deduced that when enterprises set down policies for distribution modes of output, they should link the earning of subject with his effort level in order to get better bestirring effect.

文章首先建立了数学模型,在动态情景下模拟产出分配方式对主体选择和主体规模的影响,分析了在不同的分配方式下,主体均衡努力水平的差异,以便为企业分配方式改革、激励机制完善提供理论指导;通过模型建立、均衡分析和模拟比较3个步骤,得出在制定产出分配政策时,把主体的收入与自身的努力水平挂钩,可以得到更好的激励效果的结论。

External Communication Writings whose addressees are people outside the business organization belong to external communications.

从下往上的交流,通常以报告的形式出现,提供作出明智决定的信息和建议。水平的交流发生在那些在共同水平下的职工之间。

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As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸,那乌黑、忧郁的眼睛,她便会相信,他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失

But because of its youthful corporate culture—most people are hustled out of the door in their mid-40s—it had no one to send.

但是因为该公司年轻的企业文化——大多数员工在40来岁的时候都被请出公司——一时间没有好的人选。