下水平

1.To study the expression of in the different glucose concentration with the time changing. 2.To study the effect of different concentration of Hepatocyte Growth Factor on the Integrin-linked kinase in protein and mRNA level by Mesangial Cells in high concentration (25 mmol/L) of glucose. 3.To study the effect of different concentration of HGF on the Fibronectin'secretary by Mesangial Cells. 4.To study latent therapeutic action and possible mechanism of HGF on the glomeruler sclerosis. The difference of ILK's experession on mRNA level in different concentration of glucose and HGF are detected by means of revers transcription-polymerase chain reaction,and by means of immunohistochemistry to detect their difference in protein level;the effection of HGF to the FN's secretary in high glucose are detected by means of enzyme linked innunosorbent assay.

1探讨在不同的血糖浓度下大鼠肾小球系膜细胞中ILK伴随时间变化在蛋白和mRNA水平表达的差异 2探讨不同浓度的HGF对高糖环境下(25mmol/L)大鼠肾小球系膜细胞中ILK在蛋白和mRNA水平表达的影响 3探讨不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响 4初步探讨HGF对肾小球硬化的潜在的治疗作用及其可能机制采用RT-PCR的方法分析在不同血糖浓度和不同HGF浓度下大鼠肾小球系膜细胞中ILK在mRNA水平表达的差异；采用免疫组织化学的方法直观的观察它们在蛋白水平表达的差异；采用ELISA法分析不同浓度的HGF对高糖环境下大鼠肾小球系膜细胞的FN的分泌的影响。

This paper has studied the physiological and biochemical changes during the period of forming strong buds of tree peony, and expect to provide academic basis for extending its view value and economic benefit . The main results are as the following:1 Under the field condition, Characteristics of photosynthesis of tree peony are very regular. From before blooming to the prophase of abducting buds that after blooming ,that is April,May and June,the net photosynthetic rate of tree peony is higher than the other period .It indicates that this period is very important for accumulating photosynthetic products of tree peony . So, if we want to get quality flower of tree peony ,it is very important to strengthen the management of tree peony cultivation and to ensure provide the fertilizer and water.2 From the middle ten days of June, with the time elapsing and with the environment conditions worsening, the characteristics of photosynthesis of tree peony decline deeply. But it is different among different tree peony breeds. For example , cv. Wu Long Peng Sheng can also has higher net photosynthetic rate, it indicates that cv. Wu Long Peng Sheng is super than the other two tree peony breeds when adapting the environment conditions and cv. Wu Long Peng Sheng has wide prospect to extend .3 The main factors of effecting the characteristics of photosynthesis are stomatal factor and non-stomatal factor ,that is the ability of photosynthesis of leaf cells. Under the environment of high light intensity in midsummer, if overshadow tree peony properly, then it can slow the declining of chlorophyll content, and increase the net photosynthetic rate and benefit for the accumulating of photosynthetic products.4 The content of soluble sugar in the root of tree peony is the highest among different organs during the period of forming strong buds . According to analyse ,we believe that the soluble sugar will compose starch then, and provide the solid base of material for the growth and development next year. It provide us strong academic basis of adopting the method of cultivating root and protecting root under thefield environment conditions .In other words ,it will help to increase the health level of the whole plant of tree peony and will be beneficial to forming quality tree peony flower if we strengthen the management of root. 5 To tree peony, the endogenesis hormone is important for its growth and development .In the prophase ,that is about the blooming of tree peony and the mid-summer ,IAA and ZR contents are higher and GA content is lower and its changes are very little, and the content of ABA is decreasing . This indicates that IAA and ZR act the important promote function for growth and development of tree peony in this period, and the function of GA is not important. Bur in the period of anniversary senescence of tree peony , the content of IAA and ZR begin to decrease ,while the ABA content has a certain rebound .T

本研究对几个牡丹品种壮芽形成过程中的某些生理生化变化做了研究，以期了解牡丹生长发育过程中生理生化的变化规律，探讨大田栽培条件下提高牡丹花质量的栽培措施和管理技术，为提高牡丹的观赏价值和经济效益提供理论依据，结果如下：1 大田栽培条件下，牡丹光合特性在生长发育过程中表现出极强的规律性，牡丹各品种的光合速率从牡丹开花前，到牡丹花谢后的花芽诱导期前期（6月10日之前），也就是在4、5、6月份，各牡丹品种的净光合速率相对与其他时间都维持在比较高的水平，这说明，在这段时间，是牡丹光合产物积累的重要时期，因此，要保证得到高质量的牡丹花，在这段时间加强对牡丹的栽培管理，保证这个时期的肥水供应十分重要。2 从6月中旬后，随时间的推移，环境条件的恶化，牡丹的光合性能剧烈下降，但是不同牡丹品种间仍有差异，比如，乌龙捧盛仍可维持较高的光合水平，这说明了乌龙捧盛这个牡丹品种对于环境的适应性较之其他牡丹品种高，有着广泛的推广前景。3 影响牡丹光合性能的主要因子是气孔因素和非气孔因素即叶肉细胞的光合能力，在盛夏高温高光强的环境条件下，对牡丹进行适当遮荫，能缓解牡丹叶绿素水平的下降，适当提高Pn，有利于光合产物的积累。4 可溶性糖在牡丹各品种的壮芽形成过程中，根中可溶性糖的含量在牡丹各部位中始终维持最高水平，分析认为这些可溶性糖在根系中进一步合成淀粉等储藏性物质，为来年牡丹的生长提供雄厚的物质基础，这为我们在大田栽培条件下对牡丹采取养根护根的栽培措施提供了有力的理论依据，即在大田栽培条件下，加强对牡丹根系的管理，有助于提高牡丹整株的健壮程度，有益于形成高质量的牡丹花。5内源激素对牡丹的生长发育调节作用是十分明显的，在前期，即开花前后和盛夏，IAA和ZR含量维持在比较高的水平，GA含量低且变化不明显，ABA含量逐渐降低，说明了IAA和ZR对牡丹在这段时间内的生长发育起到重要的促进作用，GA的作用不是很明显，ABA可能在高温高光强下有明显的升高，也说明了ABA的确有一些促进作用，但是到了牡丹周年衰老期，IAA和ZR的含量开始下降，ABA含量却有了一定程度的反弹，说明了IAA和ZR的作用开始降低，ABA起到了促进衰老的作用

First of all, the paper carried out test study on four two-spanned model members of FSMRCWB, including three 1/2-scaled two-storeyed models and one 1/3-scaled three-storeyed model. The paper analyzed the test results, including analysis on mechanical property of the composite wall beam under vertical loads, and failure mode, deformation capacity, aseismic performance under horizontal loads and constant vertical loads. Secondly, the nonlinear property of concrete is studed and the nonlinear models of FSMRCSS is builded by ANSYS. Last, The paper studies the result of ANSYS, which mainly includes the loading-displace curve of FSMRCSS under horizontal loads and constant vertical loads, the mechanics of arch under vertical loads lonely and under horizontal loads and constant vertical loads respectively, and status of the distribution and changing of vertical stress and cross shear on the surface of the suporting beam, and the influence of span ratio and hole.All in all, the caculating results are in good agreement with the test results.

本文首先进行了三个1/2比例的两跨两层和一个1/3比例的两跨三层的框支密肋复合墙梁的模型试验，分析了框支密肋复合墙梁在竖向荷载作用下的受力性能，以及在竖向荷载与水平低周反复荷载共同作用下的受力性能、变形性能、滞回性能、耗能系数及破坏形态；其次，研究了混凝土的非线性特性，建立了框支密肋复合墙梁非线性有限元模型，并借助大型通用有限元软件ANSYS进行计算；再则，进行了非线性有限元结果分析，主要研究框支密肋复合墙梁在竖向荷载与水平地震力共同作用下的荷载位移曲线，分析在竖向荷载单独作用下和在竖向荷载与水平地震力共同作用下墙梁的拱作用，托梁顶面的竖向应力和横向剪应力分布及变化情况，并讨论跨度比、洞口对墙梁拱作用和托梁顶面的竖向应力的影响。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

The activitys of Super Oxide Dismutase, peroxidase and Multiple Displacement Amplification of Ceratophyllum demersum L raised rapidly in the late of experiment, and the maximum value of MDA of Ceratophyllum demersum L in the eutrophic condition was 5 time higher than that in the medium nutritional condition, implied that Ceratophyllum demersum L has been stressed in the middle nutritional condition.

金鱼藻超氧歧化酶活性、过氧化物酶活性和丙二醛含量在试验后期上升速度加快，且富营养水平下金鱼藻MDA质量分数的最大值是中营养水平下最大值的5倍，表明在中营养水平下金鱼藻已经受到明显胁迫，而在富营养水平下受到胁迫更为显著。

The results showed that during the 45 days experimental time the fresh weigh of Myriophyllum spicatum L, Hydrilla verticillata L and Cerato-phyllum demersum L were 0.92 g, 0.27 g, 1.11 g and 0.56 g, 0.41 g, 0.87 g in medium nutritional condition TN 1.86 mgL^(-1; TP0.087 mgL^(-1) and eutrophic condition TN 2.47 mgL^(-1; TP0.16 mgL^(-1), respectively. The largest single plant biomass of Myrio-phyllum spicatum L and Ceratophyllum demersum L in in middle nutritional condition were over 39% and 22% higher than those in eutrophicated condition, but that of Hydrilla verticillata L was less than 51%, which shown that Myriophyllum spicatum L and Ceratophyllum demersum L grow better than Hydrilla verticillata L in middle nutritional condition.

研究结果表明，在45 d的培养过程中，狐尾藻、轮叶黑藻和金鱼藻在中营养TN1.86 mgL^(-1； TP0.087 mgL^(-1)和富营养TN2.47 mgL^(-1； TP0.16 mgL^(-1)水平下的单株最大生物量分别是0.92、0.27、1.11、0.56、0.41和0.87 g，狐尾藻和金鱼藻在中营养水平下单株最大生物量分别较富营养水平下高出39%和22%，而黑藻在中营养水平下单株最大生物量较富营养水平下低了51%，表明狐尾藻和金鱼藻在中营养水平下的生长较黑藻好。

Using panel data of listed companies, this paper analyzes it with a quantile regression approach, then draws a conclusion: the share structure and the capability of emerging interior resources effect the capital structure changeably with the different quantiles respectively. That is, for those conditions with low asset-liability ratio, the sign is positive, on the contrary, the sign is negative.

为此本文尝试着将分位数回归理论引入到回归模型中，通过对我国上市公司的相关数据进行分位数回归分析后发现，不同资产负债率水平下，企业股权结构、企业产生内部资源的能力对资本结构的影响效果不同：低负债率水平下，两者均与债务水平正相关；而高负债率水平下，又均与债务水平负相关。

The results showed that resistant gene Ht in F1 heterozygote was the most effective on controlling spots number and size; all monogene in heterozygote could produce spore by moisture culture and resistant gene Ht1 was the most effective; resistant gene Ht1 in medium level, Ht3 in high level, and Ht in low level of vertical resistance of heterozygote, were the most effective on controlling spots spread; while Ht2 in high level of vertical resistance of heterozygote was the most ineffective on controlling spots spread; and all resistant monogene in heterozygote showed superior to their parents on controlling incubation period and gene Ht was more significant than other genes.

结果表明，抗性单基因Ht处于F1代杂合状态时，控制病斑数和病斑面积的效应最强；单基因处于杂合状态时，湿培48h病斑全部能产生分生孢子，且处于同一水平抗性杂合状态时，Ht1控制产孢量的效应最强；Ht1处于中水平、Ht3处于高水平和Ht、处于低水平垂直抗性杂合状态下，控制病斑扩展效应最强；而Ht2处于高水平垂直抗性杂合状态下，控制病斑扩展效应最弱；抗性单基因处于杂合状态下，控制潜伏期多表现超亲现象，Ht控制病害潜伏期比其它单基因显著。

Based on both oblique functions and magnitude of the patterns, 59 cases of V pattern were divided into three groups and treated with three surgical methods, vertical transposition of horizontal recti, inferior oblique weakness and vertical transposition of horizontal recti combined with inferior oblique weakness.

59例V征患者根据下斜肌功能及上下25°注视时的斜视度差分别采取水平肌后徙和/或缩短+下斜肌减弱术、水平肌后徙和/或缩短+下斜肌减弱术+水平肌垂直移位术、水平肌后徙和/或缩短+水平肌垂直移位术，术前术后检查眼位及双眼视功能情况，观察三种手术方式的效果。

The flow resistance of Ice slurry increases if the pipes obliquity increases. That means the ice slurry that in the level pipe has the largest flow resistance, the reason is: the gravitation make the flow became more and more foul-up, accordingly, lead to the increase of flow resistance. Sixthly, introduced all of the preparative tasks before we began the experiment, because we met many problems during the test, we had our own methods which could solve these problems. In this paper, problems and these methods were introduced.

随着管道倾角的增大，二元冰的摩擦阻力减小，也就是说相同条件下水平管道内的摩阻最大，这是因为重力的影响导致管道内二元冰和乙二醇分层现象更加明显，从而导致紊流程度增大，最终使得摩阻增大；第六，对二元冰蓄冷空调系统运行前的准备工作进行了介绍，由于在试验过程我们遇到了很多问题，针对这些问题，我们有自己的方法和解决办法，本文介绍了这些问题和相应的解决办法。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失