下水平

METHODS: The level of intracellular calcium of human microglia grown on coverslip,which was loaded by calcium-probe,Fluo-4,and then treated in various experimental processing,was detected by confocal microscopy with time resolution mode.The binding of gp120 to human microglia was determined with confocal microscopy or flow cytometry after treatment with gp120 and stained with anti-gp120-FITC antibody.Phosphorylation of ERK within human microglia with or without gp120 stimulation was analyzed with confocal microscopy following the direct immuno-staining with anti-phosphorylated ERK antibody.

用钙离子探针Fluo-4标记粘附在盖玻片上的人小胶质细胞，运用共聚焦显微镜以荧光强度为指标实时观察各种条件下的细胞内钙离子水平的变化；用gp120处理并用anti-gp120-FITC进行染色，运用共聚焦显微镜术和流式细胞术分析人小胶质细胞与gp120结合情况；用抗磷酸化ERK 抗体免疫荧光方法进行染色，运用共聚焦显微镜术和流式细胞术进行ERK磷酸化水平分析。

These results showed that B directly involved in the polar auxin transport process.5. Under control condition, decapitation resulted in the rapid increase of cytokinins concentrations in the shoot and the release of lateral bud of pea pl

对缺硼的豌豆植株去顶也可以导致细胞分裂素水平的增加，但变化的幅度明显小于对照条件下的变化，表明由于缺硼影响了激素的水平从而减弱了顶端优势。

Methods: firstly,depanding on the methods of fcm,mtt etc,we got the best density and actiontime of adriamycin.and then,we used several depressors of different signal transduction pathways to observe the possible pathway of osteosarcoma cell apoptosis.finally, we observed different expression including phosphorylation and non phosphorylation of correlated signal molecules,by the methods of westernblot.

首先通过fcm，mtt等方法探讨引起成骨肉瘤细胞凋亡的最佳药物作用浓度及时间；在此条件下应用不同信号转导通路的抑制剂观察导致细胞凋亡的可能通路；最后应用western blotting来检测在成骨肉瘤细胞凋亡过程中相关信号分子的表达水平及磷酸化水平的变化。

2QTL model for total meat content, loin eye area and bone percentage had reached chromosome-wide level (P.05) and a significant improvement on the one-QTL.

在确认瘦肉量、眼肌面积和骨率1个QTL染色体水平显著情况下，用同一染色体2个QTL模型也达到了染色体显著水平，且比1个QTL模型解释更多的表型变异。

Huang-hua' pear fruit was a typical climacteric fruit after harvest, and the fruit senescence-related factors, such as LOX activity, 02" content, AOS activity, ACC synthase activity, ACC content, ACC oxidase activity and ethylene production, changed with peaks at 20 "C. The activities of LOX, AOS, ACC synthase, ACC oxidase and the accumulation of O2 ", ACC content and ethylene synthesis were inhibited when stored at 1癈. And the severity of flesh floss and fruit decay was lightened. Low temperature combined with polyethylene film bag packing (PEF1 and PEF2) could further decrease the rate of weight loss, flesh floss, and fruit decay and keep preferable freshness. When PEF1 and PEF2 packing fruits were transferred to shelf life at 20 "C after storage at 1癈. The activities of LOX, AOS, ACC synthase, ACC oxidase could be suppressed and the accumulation of 02 , ACC content and ethylene production were slowed down to a certain extent, especially during the shelf life after 60 d storage. PEF1 and PEF2 packing fruit could maintain higher firmness as well, and the effects became more distinct along with the storage period, there existed no significant difference between PEF1 and PEF2 treatments.

试验结果表明，在20℃下，LOX活性、O2-水平、AOS活性、ACC合成酶活性、ACC含量、ACC氧化酶活性及乙烯的生成，均呈峰形变化；1℃处理可抑制LOX、AOS、ACC合成酶、ACC氧化酶的活性和O2-、ACC的积累，抑制贮藏果实的乙烯合成，减轻果肉发绵和果实腐烂。1℃下贮藏的果实，若结合不同聚乙烯薄膜袋(PEF1和PEF2)小包装，可以进一步降低果肉发绵率和果实腐烂率，保持果实新鲜度；当果实经过一段时间贮藏后，转移到20℃下，在货架期间，PEF1和PEF2对果实成熟衰老各个相关因子均有不同程度的抑制效应，主要表现在贮藏60d后的货架期间，同时维持较高的果实硬度，对硬度的影响随着贮藏时间的延长愈加明显，PEF1和PEF2处理效果之间无显著差异。

Between the top board of the support and girder, between the bottom board and frusta when the level power is three times of the design horizontal supporting capacity.

充分保证了支座上座板与梁体、下座板与墩台之间在水平力为设计水平承载力三倍时无相对位移。

The measurement results show that the general characteristics of the present crustal stress field of the project area are as follows: the horizontal principal stress is the maximum principal stress, and the preferred orientation of the maximum horizontal principal stress is NW-WNW, the maximum principal stress of surrounding rocks of the plant buildings and high-pressure branch tunnel area is generally 14.5±0.5 MPa and the minimum principal stress is 9±0.5 MPa; the dead oad-bearing capacity of surrounding rocks is 10—14 MPa; and the surrounding rocks are essentially impermeable at high pressures of 8—10 MPa.

测量结果表明，工程区现今地壳应力场的总体特征为：水平主应力为最大主应力，最大水平主应力的优势方向为NW-NWW向；厂房和高压岔管区围岩的最大主应力值一般为14.5±0.5MPa，最小主应力值一般为9±0.5MPa；围岩自身的抗载强度一般为10～14MPa；在8～10MPa高压力作用下围岩基本不透水。

Thirdly, on the basis of the refractive index function around a hot-rolling bar obtained from experiments, the law of the light-ray propagation traces and the light-ray deflection around a high-temperature bar (stationary and in motion, respectively) is studied by the numerical computing of the light-ray differential equations.

作者用光学干涉和热电偶测温法，系统地对直径从35-86mm、壁温从500-1170℃的水平圆柱在自然对流及受迫对流状态下、周围折射率场或温度场进行了实验研究，得到了高温差水平圆柱对流换热的实验关联式。

The results showed compared with the native state the shear strength of rockmass under the saturation state decreased by 11.17%, cohesion force decreased by 16.09%, internal frictional angle decreased by 10.02%.(3) 3D-σFEM numerical simulation software is used to simulate the slope stability on the native state and saturation state. Compared with the nature state the displacement of the discontinuity and stress concentration in the saturation state is largened clearly, the results showed slope stability under the rainfall is weakened.

更多含有天然结构面的岩体试件进行了不同含水量下的强度试验，试验结果证明在岩体试件处于饱水状态时的结构面抗剪强度比天然状态下平均下降了11.17%、粘聚力平均下降了16.09%、内摩擦角平均下降了10.02%；(3)采用3D-σ有限元数值软件对K86+180～K86+470段岩质边坡进行了三维有限元数值模拟，并且分天然状态分阶段开挖和饱水状态分阶段开挖两种不同状况进行了稳定性分析，从分析结果可以看出与天然状态相比，饱水状态下软弱层水平方向位移明显增大，坡脚及软弱层特别是软弱层临空面附近的剪应力值显著增大，并且其安全系数与天然状态相比也显著减小，说明在有大的降雨发生当岩体处于饱水状态时边坡整体稳定性被削弱，边坡失稳可能性增大。

are gained that after widening, the settlement of two sides of the old road pavement is contrary, cross slope of old road is different, horizontal displacement of the old road pavement is minishes, the area of maxium settlement, maxium lateral displace and disease of old road is different as before widening.

分析结果指出：在拓宽段作用下，靠近老路中心一侧老路路面沉降逐渐减小，远离老路中心一侧沉降逐渐增大，相应横坡也在发生变化；老路路面的水平位移有较大幅度的减小；而且老路最大沉降、最大水平位移、最大剪应力及最易发生病害的位置与拓宽前也发生了较大的变化。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失