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The purpose of the experiment is to realize the prokaryotic expression of β-glucosidase gene from Acetobacter xylinum ATCC 23769, which will be used to produce enzyme preparation so as to develop the efficiency of extracting resveratrol from plants. Aspergillus niger β-glucosidase gene is cloned both at DNA lever and cDNA lever, which will lay a foundation for further research about its expression in Saccharomyces cerevisiae in a secretory form with the aim of increasing resveratrol contents in wines.

本实验实现了木醋杆菌β-葡萄糖苷酶基因的原核表达,目的是为了生产粗酶制剂,用于提高从植物中提取白藜芦醇的得率;分别在DNA和cDNA水平从黑曲霉中克隆β-葡萄糖苷酶基因,为下一步在酿酒酵母中实现分泌表达以提高葡萄酒的白藜芦醇含量的研究奠定基础。

According to the arra ngem ent of phloem and xylem, shape of xylem and the Arranging way of xylem rays, the midrib-bundles were divided into five forms :(1) amphicribral round bundle;(2 ) pinnate-ray amphicribral reniform bundle;(3) fan-ray amphicribral reniform b undle;(4) almost-amphicribral bundle;(5) collateral bundle.

根据中脉维管束结构特点:木质部与韧皮部的位置,木质部的形状,木射线排列的方式,可分为5个类型:(1)圆形周韧维管束;(2)羽线肾形周韧维管束;(3)扇线肾形周韧维管束;(4)近周韧维管束;(5)下韧维管束。

This study investigated the effect of gibberellin (GA3) on the change of part of signal substances in the Japanese aralia seed dormancy-breaking process under fluctuating temperature.

研究了在变温条件下赤霉素(GA3)对辽东楤木种子解除休眠过程中部分信号物质变化的影响。

Aralia has good economy values;it could be used for eating,drug,landscaping and industry material etc.however,wildlife resource iS close to perdition by over collection for 10ng time,So it is necessary to probe into artificial breeding pattern to ensure this resource can be used constantly.

楤木有较好的经济价值,可供食用、药用、园林绿化、工业原料等。野生资源在人们长期的过度超采下已濒临枯竭,因此,探索人工繁育方式以保证资源的可持续利用势在必行。

The paper carries on qualitative analysis to the acetone extractives before and after discoloring, shows the main components of the extractives and then takes comparison. The results shows :①The hydroxyl is oxidized and then shifted to the carbonyl,carboxyl, ester and ketone groups during inducing discoloration under the pretreatment conditions,which makes the wood color deep;② There are plenty of auxochromic groups in the acetone extractives of silver chain, the interaction between the auxochromic groups and the chromophoric groups makes the wood color deep.

摘 要:本文通过对刺槐变色前后丙酮抽出物的定性分析,得出变色前后丙酮抽出物的主要成分,进行对比分析,初步得出抽出物对诱发变色的作用为:①变色前后羟基在预处理条件下氧化成羰基,羧基,酯基和酮基等官能团,使木材颜色加深;②刺槐丙酮抽出物的主要成分中含有大量助色基,这些基团会与木素中的发色基团相互作用,使木材颜色加深。

The results showed a betulin yield of 25.43%, a purity of 59.19% was achieved from 1g powder of B. platyphylla bark under the extraction temperature of 72℃, microwave power 67W, and extraction time 100s.

试验结果表明,1g白桦树皮粉末,在提取温度72℃、微波功率67W,提取时间100s的条件下,微波辅助提取的桦木醇收率达到25.43%,纯度达到59.19%。

Paul Blart: What are you trained to doNothing...

保罗·布拉特:&你所受到的训练中这种情况下应该怎么办?。。。木油这类训练。&

The seedlings could be regenerated naturally under forest canopy and are considered as shade-tolerance species. For exploration the adaptability strategy of Chamaecyparis obtusa var.

小苗可於冠层孔隙下下种更新成苗,而被视为耐荫性树种,然对於已更新成长为幼木阶段的台湾扁柏林木,其对现有林分环境的生长调适能力的暸解尚有限。

Lmt.The characteristics and novelty in this dissertation was obvious in the following aspects: cofermentation of glucose and xylose to ethanol by recombinant yeast S. cerevisiae ZU-10, ethanol production from hydrolysates of cellulose and hemicellulose in corn stover by recombinant yeast S. cerevisiae ZU-10, and integration of key technologies in ethanol production from lignocellulosic biomass. These results were meaningful not only in academic research, also in utilization of renewable lignocellulosic biomass, decreasing the production cost of fuel ethanol , and alleviating the world grain crisis.

本文在基因重组酵母对葡萄糖和木糖的共发酵、利用基因重组酵母厌氧条件下发酵玉米秸秆纤维素和半纤维素水解液生产乙醇、以及利用木质纤维原料生产乙醇的关键技术集成等方面的研究具有明显的特色与创新,相关研究结果不仅有一定的学术价值,而且在促进可再生木质纤维素资源的转化利用、缓解人类的粮食危机、降低燃料乙醇的生产成本等方面具有重要的现实意义。

UV-B absorbing capacities of methanol and cell-bound alkali extractable phenolics were measured in leaves of potted one-year-old seedlings of Pinus massoniana, Schima superba, Cryptocarya concinna, Castanopsis fissa and Castanopsis chinensis to investigate the UV-B protecting strategy in plants exposed to full sunlight.

通过测定马尾松和荷木,及黄果厚壳桂、藜蒴和锥树叶片中甲醇提取物和细胞壁碱性酚类提取物中UV-B吸收物质的含量,以及叶片叶绿素含量和比叶面积,研究全日光强下植物对UV-B的防护策略。

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