下丘脑的

Through immunocytochemistry study, we found that the α3 and/or α5 subunits of nicotinic acetylcholine receptor are predominantly expressed in the oxytocinergic neurons. Our finding is quite different from the conclusion of the British scholar Dr. Mason's work in 1980s. We also found in this study that, the α3 and/or α5 subunits of N-AChR can also be expressed on the axons of oxytocinergic neurons, and these immunoreactive axons could be seen projecting to the median eminence and further to the posterior end of neurohypophysis. In the hypothalamus of the 7-day postnatal pup rats, the expression patterns of α3 and/or α5 subunits of N-AChR are similar to that in adult rats.

我们用免疫组化的方法发现，α3和/或α5型烟碱型乙酰胆碱受体(Nicotinic acetylcholine receptor，N-AChR)亚单位在大鼠下丘脑OT能神经元中呈优势性的表达，这与上世纪八十年代英国学者Mason类似工作的结论不一致；而且我们还发现α3和/或α5型N-AChR亚单位还表达在OT能神经元的轴突，此阳性免疫反应的轴突一直延续到正中隆起和神经垂体的末端；在新生7天大鼠的下丘脑，α3和/或α5型N-AChR亚单位的分布类似成年鼠。

Methods ①In situ hybridization was applied to observe the changes ofhypothalamic CRHmRNA positive cells after PX and melatonin supplementation;②Immunohistochemistry was used to observe the changes of CRH positive cellsin hypothalamus and CRH staining in median eminence of neurohypophysis after PXand melatonin supplementation;③ELISA was applied to detect the changes of CRHconcentration in serum after PX and melatonin supplementation.

①运用CRHmRNA 原位杂交的方法来观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRHmRNA 阳性细胞的变化；②应用免疫组织化学染色的方法观察松果体摘除及补充外源性褪黑素后下丘脑室旁核CRH 阳性细胞和神经垂体正中隆起CRH 阳性染色的变化；③运用ELISA 法检测松果体摘除及补充褪黑素后血清CRH 浓度的变化。

Objective To study the morphological and functional effect of pinealectomy on CRH neurons in paraventricular nucleus of rat hypothalamus as well as thefunction of melatonin supplementation, enrich the research of the effect of pinealbody and melatonin on hypothalamic-pituitary-adrenal axis,provide theoreticalevidence of the effect of pineal body and melatonin on immune function andimmune-associated disease such as tumor and autoimmune disease.

目的： 研究松果体及其褪黑素对大鼠下丘脑室旁核CRH 神经元形态和功能的影响以及补充褪黑素的作用，丰富松果体及褪黑素对下丘脑——垂体——肾上腺轴影响的形态及功能方面的研究，为进一步揭示松果体及褪黑素对免疫功能以及免疫相关性疾病如肿瘤、自身免疫性疾病等的影响机制提供理论依据。

For example, prenatal fluoxetine exposure significantly altered the density of 5-HT transporters in subregions of the hypothalamus (dorsomedial nucleus,-21%; lateral hypothalamus,+21%), hippocampus (CA2,+47%; CA3,+38%), and amygdala (basolateral nucleus,+32%; medial nucleus,+44%) in prepubescent offspring.

比如，孕期氟西汀的使用显著地改变了青春期前后代大鼠的下列区域的5-羟色胺转运体密度：下丘脑（背内侧核，-21%，外侧下丘脑，+21%），海马（CA2，+47%；CA3，+38%）和杏仁核（底侧核，+32%，内侧核，+44%）。

Methods The depuratory-type and delactation male-rat was divideinto five groups, normal control、obesity control、catgut implantation at acupoint、drug implantation at acupoint and sibutramine.

将SD清洁型断乳1周的雄性大鼠分为五组，分别为正常对照组、肥胖对照组、普通埋线组、药物埋线组和西布曲明组，观察其治疗前后的体重、李氏指数、体脂变化以及血清、下丘脑中瘦素、胰岛素的变化、下丘脑瘦素受体基因表达的变化。

However, recent neuroanatomical researches have revealed direct and bidirectional connections (i.e., the cerebellar-hypothalamic neural circuits) between the cerebellum and the hypothalamus, the high autonomic center for regulation of the visceral functions.

一般认为小脑是一个皮层下的运动调节中枢，但近年来的神经解剖学研究揭示小脑与内脏功能调节中枢下丘脑之间存在交互的直接纤维联系，即小脑－下丘脑神经环路。

To observe the change of behavior index and mensurate the contents of plasma CORT, CRH and ACTH by using radiation immunity methods, and observed the change of immunity function by testing IL-2 and lymphocyte multiplication produced by spleen cell. At the same time, the author examined the change of thalamencephalon CRH mRNA and pituitary ACTH mRNA.

观察行为学指标的变化，利用放免法测定血浆皮质酮、下丘脑促肾上腺皮质激素释放激素和血浆促肾上腺皮质激素的含量，并通过脾细胞产生白细胞介素2（IL-2）水平和淋巴细胞增殖试验观察大鼠免疫功能的变化，同时检测大鼠下丘脑CRH mRNA和垂体ACTHmRNA含量变化。

Moreover, three prescriptions could reduce excessive expression of CRH mRNA in thalamencephalon and ACTH mRNA in pituitary. It show three prescriptions could adjust the abnormity variety of the HPA of the chronic stress rats.

四君子汤、四逆散及甘麦大枣汤均可提高慢性应激大鼠的免疫功能，不同程度地降低大鼠血浆ACTH、下丘脑CRH及血浆CORT的含量，并可降低慢性应激大鼠下丘脑CRH mRNA和垂体ACTH mRNA的过量表达。

Experiment 1, let rats acquire taste preference to quinine solution, and take account of β-EP-immunoreactive neurons in different nucleuses of hypothalamic areas, separately adopted taste preference learning training and immunohistochemistry; Experiment 2, Added banana essence into quinine solution to discuss the connection between flavor preference learning and the expression of β-EP; Experiment 3, To research endogenous opiate like substances contribute to the establishment of CTP and influence the behavior of taste preference by naloxone was i.p.

实验一，采用味觉嗜好学习训练和免疫组织化学方法，让大鼠获得对奎宁的味觉嗜好，计数了下丘脑不同核团内β-内啡肽免疫反应细胞数；实验二，向奎宁溶液中加入香蕉气味，探讨风味嗜好学习与下丘脑β-内啡肽表达的关系；实验三，腹腔内注射纳络酮，探讨内阿片肽对CTP建立及味觉嗜好行为表达的影响。

In order to study the expression of Gonadotropin-Releasing Hormone in the hypothalamus of estrus milk goat.

为了研究促性腺激素释放激素在发情期奶山羊下丘脑中的表达特点，采用超敏的免疫组化链霉素抗生物素蛋白-过氧化物酶法研究GnRH免疫反应神经元在发情期山羊下丘脑中的分布。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。