下丘脑的

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, which indicates PX and melatonin supplementation have no significant effect on CRH levels.

结果： ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低，提示松果体摘除后下丘脑CRH 神经元的表达增强；②松果体摘除大鼠补充褪黑素后，下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复，不过不论是低剂量组还是高剂量组，也不论是术后4 周还是术后8周，均不能恢复至正常；③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低，提示下丘脑CRH 神经元释放CRH 增加；④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性，表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

Results ①Compared to sham group, ISH and IHC of rat hypothalamic CRHshow positive cells numbers increase, positive areas enlarge and average gray scaledecreases, which reveals the expression of hypothalamic CRH neurons enhances afterPX;②The enhancement of ISH and IHC of hypothalamic CRH in PX rats aftermelatonin supplementation dose-dependently recovers, but it can't return to be normaleither in high dose group or in low dose group and either at 4th week or at 8th week;③IHC of CRH in median eminence of neurohypophysis shows positive areas enlargeand average gray scale decreases, which reveals the releasing of CRH fromhypothalamic CRH neurons increases;④ELISA of CRH in serum shows thedifferences of CRH concentrations among all groups are not significant, whichindicates PX and melatonin supplementation have no significant effect on CRH levels.

结果： ①松果体摘除后大鼠下丘脑室旁核CRH 原位杂交和免疫组化的结果与假手术对照组相比均显示阳性细胞数增多、阳性面积增加、平均灰度降低，提示松果体摘除后下丘脑CRH 神经元的表达增强；②松果体摘除大鼠补充褪黑素后，下丘脑CRH 原位杂交和免疫组化染色增强呈剂量依赖性恢复，不过不论是低剂量组还是高剂量组，也不论是术后4 周还是术后8周，均不能恢复至正常；③松果体摘除后神经垂体正中隆起CRH 免疫组化结果显示阳性面积增加、平均灰度降低，提示下丘脑CRH 神经元释放CRH 增加；④ELISA 法检测血清CRH结果示各组血清CRH 浓度差异没有显著性，表明松果体摘除及补充褪黑素对血清CRH 水平没有显著影响。

Which may be useful in the study of insulin signaling transduction pathway in the brain.methods:use immunohistochemical techniques to investigate the neuronal distribution of insr、irs-1 and irs-2 proteins in rat brain with polyclonal insr、irs-1 and irs-2 antiserum.results:insr、irs-1 and irs-2 are widely distributed in the brain of normal adult rat ,especially in the cerebral cortex 、hippocampus and numerous hypothalamic nuclei including the arcuate nucleus、ventromedial nucleus and paraventricular nucleus which are crucial for the control of food intake.conclusion:insr、irs-1 and irs-2 are widely distributed in the brain of normal adult rat, suggesting that they may play an important role in the normal function of central nervous system.

应用免疫组织化学染色的方法观察正常成年大鼠脑中insr、irs-1、irs-2的分布。结果：insr、irs-1、irs-2在正常成年大鼠脑中广泛分布，在与学习记忆和能量代谢相关的脑区如大脑皮质、海马、丘脑和下丘脑的部分核团均有表达。结论：insr及irs-1、irs-2在正常成年大鼠脑中广泛表达，但表达部位不完全一致，提示它们在大鼠中枢神经系统的正常功能活动中可能发挥不同的作用。

T was later transiently expressed in pharyngeal arches, and in pectoral fin buds. IGFBP-3 mRNA is predominantly and persistently expressed in the developing otic vesicle. IGFBP-3 is also expressed in optic stalk, retina, pineal gland, tegmentum in midbrain, anterior pituitary and midline floor of hypothalamus.

GFBP-3 mRNA 持续而强烈地表达在发育中的内耳，它也表达在眼睛的视杆和部分视网膜细胞，松果腺以及脑垂体前部、中脑脑盖和下丘脑的基底中央等脑组织。

Methods The model of middle cerebral artery occlusion was set up in mature rats using the technology of in situ hybridization and radioimmunoassay to dynamically observed and to quantitatively analyse the expression levels of CRH messenger ribonucleic acid in the limbic systen and the levels of adrenocorticotropic hormone in plasma.

利用鼠大脑中动脉阻塞模型，应用原位杂交和放射免疫分析法，监测对照组和缺血组鼠脑颞叶、海马和下丘脑的CRH mRNA转录水平和血浆ACTH的活性变化。

After the termination of the model,kill the mouse,detect NPY and then see the content of NPY in the Hypothalamus of the mouse significantly lower.3.8g/kg(it is valid dose proved by pharmacodynamic experiment,about 9 times of human dose) of Shanzha Maiya granula and 13.6g/kg(15 times of human dose) of Decoction of Xiangsha six Mild Drugs can play a certain role in treating the animal model of child apositia resulting from leptin,fail to increase the animal\'s food-intake,but can increase its weight to some extent,Decoction of Xiangsha six Mild Drugs can increase the content of NPY in the Hypothalamus of the mouse.4.set up quality standard of qualitatively and quantitatively detecting Shanzha Maiya granula:detecting the content of aurantiamarin as a main component of Chenpi in Shanzha Maiya granula through the method of high performance liquid phase,qualitatively identifying Shanzha,Maiya,Chenpi through the method of plate chromatography.

造模结束处死大鼠，放射免疫法检测NPY，结果造模大鼠下丘脑NPY含量显著降低(正常组下丘脑NPY平均含量为22.2ng/ml，模型组下丘脑NPY平均含量为14.8ng/ml，P＜0.05)。3。山楂麦芽颗粒8g/kg(药效学实验有效剂量，临床用量的9倍)和香砂六君子汤13.6g/kg(临床用量的15倍)对侧脑室注射瘦素致厌食症大鼠有一定治疗作用，虽没有提高动物进食量，但在一定程度提高动物体重，其中香砂六君子汤能提高大鼠下丘脑NPY含量(模型组下丘脑NPY平均含量为14.8ng/mL，香砂六君子汤组NPY平均含量为20.3ng/mL，P＜0.05)。4。

Results A dense accumulation of fos and jun positive neurons exhibited in the FC, temporal cortex, CA1, DG, PVN, the supraoptic nucleus and the dorsomedial nucleus.

结果 两种方式应激结束后3h，脑皮层的额叶和颞叶、海马的CA1和DG及下丘脑的PVN、视上核、背内侧核均有较强的c-fos和 c-jun表达。

Other brain regions showing increasedpostsurgical activity were the contralateral parietal cortex,bilateral pulvinar and ipsilateral medial dorsal nucleus ofthe thalamus, contralateral putamen, contralateral superiortemporal gyrus, ipsilateral fusiform gyrus, ipsilateral posteriorlobe, and contralateral anterior cerebellar lobe.

术后活动性增强的大脑区域还有对侧的颅顶部的大脑皮层，两侧的丘脑后结节和下丘脑的背内侧核，对侧的壳核，对侧的上部颞侧脑回，同侧的梭状回，同侧的后叶和对侧的小脑前叶。

Low dose Yiweitang group; 4. Diethylstilbestrol group; 5. Model control group. After 4 weeks intervention, get the left ovarie, a part of hypothalami and pituitary gland from head-off rat, and observe expression of ER, FSHR and GnRH by immune histochemistry method . Then extract RNA from the rihgt ovarie and the rest hypothalami and pituitary gland, so as to measure the expression of GnRHmRNA 、 ERmRNA 、 FSHRmRNA and P 16mRNA by RT-PCR. And the cerebrum were made to homogenate to examine SOD, GSH-PX and MDA.

各组实验动物灌药4周后，断头处死，取部分下丘脑、垂体及左侧卵巢组织固定切片，免疫组化法检测 ER 和 FSHR 表达、 GnRH ；取剩余部分下丘脑、垂体及右侧卵巢组织提取总 RNA ，用于 RT-PCR 检测下丘脑 GnRHmRNA ，下丘脑、垂体的 P 16mRNA 及下丘脑、垂体、卵巢的 ERmRNA 、 FSHRmRNA 基因表达；另外取部分大脑组织制作脑组织匀浆，用于 SOD 和 GSH-PX 酶活力以及 MDA 含量的测定。

In addition to the pons positive neurons were also found in cerebellum, most nucleus of thalamus and hypothalamus, and corpus striatum of chicken.

鸡脑阳性神经元除广泛分布于脑干外，小脑、丘脑的多数核团、下丘脑的背侧核、腹侧核和后核以及大脑纹状体等多数脑区也有一定分布。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。