上首

On productions at the National Theatre, Skini and Nea Skini theatres, Aplo Theatro, Epochi theatre, Amphitheatro, etc., of plays by Shakespeare, Goldoni, Griboyedof, Turgenief, Chekhof, Gorki, O'Neill, Tennessee Williams, Arthur Miller, Pinter, Mammet, Orton, Aristophanes, Hourmouzis, Loula Anagnostaki, G.Dialegmenos, Euripides etc.At the cinema: Since 1983 she has been a permanent collaborator with Theo Angelopoulos, composing music for his films Voyage to Cythera, The beekeeper, Landscape in the Mist, The Suspended Step of the Stork, Ulysses' Gaze and the Cannes Festival Golden Palm award-winning film, Eternity and a Day. She has also composed music for films by Dimitris Mavrikios, Takis Kanelopoulos, Tonia Marketaki, Christoforos Christofis (Wandering / Rosa), Lefteris Xanthopoulos, Giorgos Koras Margarethe von Trotta (L' Africana), etc.

然后她回到雅典，上电台，办民族音乐试验室，1979年被她视为命运的转折点，她开启了电影作曲的职业生涯，合作者是希腊诗人兼新电影流派的导演Chistoforo Chillstofis，1982年Eleni Karaindrou因前者的影片"芳名罗莎"在塞瑟隆尼基电影节上获得原创音乐奖，颁奖人正是评审团主席Theo Angelopoulos，他们的合作也由此开始，以后的九年可谓硕果累累，两个人物以类聚，谁都没有辜负了彼此间的厚望。80年代安氏创作了他的"漂泊"三部曲，当首篇"塞瑟岛之旅"告竣之后，安氏在访谈录中称赞Eleni正是他所需要的那种博采众长且深具古典音乐实力的最佳搭档，Eleni不仅创作电影音乐，而且也涉足希腊的先锋戏剧，她的第二个丈夫就是一位戏剧导演，Eleni迄今已为35部剧本谱写了音乐。

This time the title is totally in the mode of so-called "Yasagure" enka, especially lyrics by Aku Yu --"Lingering Words About Hell of Love", similar to other hits by same lyricist.

上面说到炫技，其实这首才最炫技。歌词执笔是阿久悠，结构及词意上都跟其在之前两年为北原ミレイ写下的得意作《ざんげの値打ちもない》同出一辙：四季流转，寂寞女人失爱受伤，爱变恨，恨变地狱。B面作《ふるさともんめ》的作词则是画师上村一夫本人。

Alessandra Ambrosio was born in Brazil in 1981. She was just 8 years old when she decided she wanted to be a model after seeing a magazine picture of Dutch supermodel Karen Mulder. She appeared in the Victoria's Secret Fashion Show for the first time in 2000. By 2004, she was selected as the first spokesmodel for the Victoria's Secret "PINK" line and became a contracted "Angel".

亚历山大·安布罗休1981年生于巴西。8岁那年，安布罗休在杂志上看到了荷兰超模凯伦·穆德的照片，便下定决心要当模特。2000年，她第一次出现在"维多利亚的秘密"时装秀上，2004年，她被选为"维多利亚的秘密"PINK系列首个代言模特，并成为"签约天使"。

Analysis based on the experimental results demonstrated the plausible reaction mechanism is that vit C first converts Cu(superscript 2+) to its reduced state and vit C becomes vit C radical itself, thereby stimulating the formation of peroxyl and alkoxyl radicals, and bimolecular reaction of peroxyl radicals results in (superscript 1)O2 production in the systems studied.

根据实验观察的结果，分析了这一体系中(上标 1)O2形成的可能途迳，认为首先是维生素C将Cu(上标 2+)转变为还原态，而自身失去一个电子转变为维生素C自由基，从而刺激了过氧自由基和烷氧自由基的形成，过氧自由基的双分子反应很可能就是体系内(上标 1)O2产生的反应机制。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

Reform and opening up, China's rapid development of the aerospace industry, China is now in the history of a glorious page. In 2003, the successful launch of Shenzhou V, Yang uncle realized the dream of the Chinese nation to fly the Millennium; in 2008, China's first lunar exploration satellite "Chang'e I" flew up into space, to the moon, and Chang'e Yutu visit; the same in 2008 years, the Shenzhou VII launch翟志刚to become the first history of China's spacewalk astronauts.

改革开放，使中国的航天事业迅速发展，中国在历史上刻下了辉煌的一页。2003年，神舟五号发射成功，杨利伟叔叔实现了中华民族的千年飞天梦；2008年，中国的首颗探月卫星&嫦娥一号&飞上太空，到月球上去拜访嫦娥和玉兔；同样是2008年，神舟七号升空，翟志刚成为中国历史上首位进行太空行走的宇航员。

Happiness is mental before it is physical ,in order to physically happy to the full ,you must first feel at ease and be free of worry .it is better to eat without meat than to have a load on your mind.

快乐首先是精神上的，然后才是身体上的，要求身体充分享受快乐，先要精神上悠然自得，心无挂虑，宁可食无肉，不要心有石。

The hull body fractured and broken at about 1/3 of the length from the stem and in way, the shell plating, upper deck plating, inner bottom plating and internal structural members within the scope of about 10m torn or destroyed; the remaining shell plating, upper deck plating, inner bottom plating and internal structural members seriously indented and buckled or bent and deformed in different degrees.

船体从船首起约 1/3 处折裂破断，该处船壳板，上甲板，内底板，内部构件在约 10 米范围内撕裂或损毁；其余船壳板，上甲板，内底板，内部构件严重凹陷，皱折或不同程度上弯曲变形。

The sin of Judah is written with a pen of iron, and with the point of a diamond: it is graven upon the table of their heart, and upon the horns of your altars

正在阅读：《耶利米书》第17章，本书共52章和合本首章上一章下一章尾章大小原犹大的罪，是用铁笔、用金钢钻记录的，铭刻在他们的心版上和坛角上。

Played in all three levels during year and reached SF in San Antonio Futures and SF at Belo Horizonte and Buenos Aires Challengers… Made ATP debut by qualifying for AMS Miami where he lost to former No.

2000年在青少年，挑战赛和ATP巡回赛上都有出色表现。在圣·安东尼奥的青少年组比赛进入半决赛，在巴西和阿根廷的两站挑战赛上也进入了半决赛。在迈阿密大师赛上通过资格赛晋级正赛，这是他的首场ATP比赛。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！