上胶

In this paper we combined three chromatographic separation and purification technique such as affinity chromatography, ion exchanger chromatography and hydrophobic interaction chromatography to develope a new technology of stimutaneous extraction of three enzyme from pancreatin. We optimized the technology by studying the methods of purification and assured the technology as: The crude extraction from the dissolution of Pancreatin is directly absorbed on the DEAE gelose fast flow columnEquilibrating buffer is 0.01mol/L NaoAc-HoAc buffer(pH4.5; eluting buffer is 0.2～0.35mol/LNaCl in 0.01mol/LNaoAc-HoAc buffer (pH4.5), and then be eluted by two steps to acquire the peak of kallikrein.The solution which can"t be adsorbed by DEAE gelose fast flow column is adsorbed on affinity chromatographic column Equilibrating buffer is 0.01mol/LTris-HCl buffer(pH7.5, eluting buffer is 0.5mol/LNaCl in 0.01mol/Ltris-HCl buffer(pH7.5)and then be eluted by one step to acquire the peak of trypsin.The solution which can"t be adsorbed by is pretreated with 30%～80%(NH_4)_2SO_4 fractional precipitation, the deposition of the precipitation is dissolved to beabsorbed on phenyl gelose fast flow columnhydrophobic interaction chromatography condition is Equilibrating buffer is lmol/L(NH_4_2SO_4 in 0.01mol/LNaoAc-HoAc buffer(pH4.5), eluting buffer is 0～0.6mol/L(NH_4)_2SO_4 in 0.01mol/LNaoAc-HoAc buffer (pH4.5) and then be eluted by two steps to acquire the peak of chymotrypsin.

本研究考察了各种纯化方法，将离子交换层析、亲和层析和疏水层析三种分离纯化法相结合，建立了激肽释放酶、胰蛋白酶和糜蛋白酶三酶的联产工艺：胰酶用pH4.5醋酸缓冲溶液提取后，粗提液直接上DEAE-琼脂糖快胶柱吸附平衡缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5，洗脱缓冲液：0.01mol／LNaoAc-HoAc缓冲液(pH4.5)含0.2～0.35mol／LNaCl分两步洗脱，收集激肽释放酶的洗脱峰；DEAE-琼脂糖快胶的未吸附液上亲和层析柱分批吸附平衡缓冲液：0.01mol／LTris-HCl缓冲液(pH7.5，洗脱液：0.5mol／LNaCl溶液，一次洗脱，收集胰蛋白酶洗脱峰；最后，亲和层析未吸附液用30％～80％硫酸铵分级盐析处理，沉淀溶解后用上苯基—琼脂糖快胶吸附平衡缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5含1mol／L(NH_4)_2SO_4，洗脱缓冲液：0.01mol／LNaAc-HAc缓冲液(pH4.5)含0～0.6mol／L(NH_4)_2SO_4，分两步洗脱，收集糜蛋白酶的洗脱峰。

Key components installed ink, adjust the blade support shaft section of copper Cap nut, the blade front-end on either side of half-round slot is placed in the kuyto specialadhesive Groove at the antileakage gum, tighten the screws, the installation is complete with rubber extrusion antileakage blade, the two-blade antileakage glue between the slot from semicrcular extpuded, producing gum in the column so that you can more effectively prevent ink seepage.

在安设不朱键组件时，调动不朱刀支架轴段的铜螺帽，不兵不朱刀前端两侧的半圆槽安插在托不活胶槽处的防漏胶上，拧不松变动螺丝，安设达成后用不朱刀挤压防漏胶，不兵两不朱刀之间的防漏胶从半圆槽洋挤出，爆发胶柱，这样不离可以更灵验不天避免油不朱渗漏。

From the angle of engineering application, this article analysed the corrosion characteristics and damage causes of the current structural used concrete in the region of Hangzhou gulf, on the basis of on-site survey of all the Marine concrete projects in that area. Taking HPC used in Marine as research objective, this article put great emphasis on the influence of the composition cementing material system to concrete performance, and make a preliminary probe into the action mechanism of the composite effects of various mineral materials in improving the performance of concrete by laser measuring size machine and EMPA. Through experiments, the article discussed how chloride ingression in concrete will be effected by such factors as exposure time, chloride binding, environment temperature, stress and cracks conditions; and also analysed the symptoms of these factors in the model of concrete service life prediction by conference to all related research reports both home and aboard. On the foundation of abovementioned research, this paper brought up an engineering model of service life prediction of marine concrete, which is based on chloride-induced corrosion.

本文从工程应用的角度，在对杭州湾地区既有海工混凝土工程作实地调研的基础上，研究分析杭州湾地区既有海工混凝土结构的腐蚀特点和破坏原因；以海工高性能混凝土为研究对象，着重研究复合胶凝材料体系对混凝土性能的影响，并应用激光粒度仪、EMPA等分析手段，就复合胶凝材料的作用机理进行初步探讨；试验研究混凝土暴露时间、胶凝材料对氯离子的吸附、环境温度、应力和裂缝状态等因素对混凝土中氯离子迁移运动的影响，并结合国内外相关研究报导，探讨这些因素在混凝土使用寿命预测模型中的表征；在上述研究基础上，提出以氯离子侵蚀为基础的海工混凝土使用寿命预测的工程应用模型。

The heat generation curves of seven rubber materials used in tires with the changes of temperature have been obtained by using Dynamic Mechanics Analysis technique on NETZSCH DMA242 instrument, knowing that the heat generation of every other rubber materials except inner liner rubber changes lightly alone with the temperature within 10℃～80℃. According to the Fourier law, the heat transfer coefficients of tire rubber are measured by stable method on self-made experiment instrument. The coefficients changes lineally within 20℃～80℃ and the relation formulas λ=λ〓+bt are given out attached with λ〓 and b. The specific heat of tire rubber are measured on Differential Scanning Calorimeter NETZSCH DSC204 and the relations with temperature are linear and the formula is c=c〓+bT, where c〓 and b gained from experiment result data are presented in the paper. The heat convection coefficients on the surface of rolling tire are measured according to the principle of heat-mass transfer analogy principle after simplifying rolling tire into rotating disk with straight movement on wind channel. The relation with rotation speed of disk is linear as h=5. 399+0.0258n and the relation with straight line speed of tire is linear too, h=

论文采用动态力学分析技术，使用NETZSCH DMA 242型实验仪，获得了轮胎用七种胶料的生热率随温度变化曲线，确知除内衬层胶以外，其它各种胶料在一般胎温范围内（10℃～80℃）的生热率随温度变化不大；轮胎胶料的导热系数根据傅立叶定律的基本原理，采用稳态法在自行搭建的实验台上进行测试，结果表明，材料的导热系数在20℃～80℃的温度范围内随温度呈线性变化，论文给出了各种材料导热系数的线性变化的关系式λ=λ〓+bt及其λ〓和b的值；轮胎胶料的比热运用差式扫描量热原理，在NETZSCH DSC204型DSC实验仪上进行测试，结果表明，胶料的比热随温度的变化呈线性变化，其关系可用c=c〓+bT表示，文中给出了根据实验数据拟合的c〓、b的值；对轮胎表面的对流换热系数的测定，本文先将滚动的轮胎简化为有直线运动的旋转圆盘，而后采用萘升华热质比拟技术，在风洞中测量了滚动轮胎表面的对流换热系数，结果表明，圆盘表面对流换热系数与转速呈线性关系，即h=5.399+0.0258n，轮胎表面对流换热系数与其直线运动的速度呈线性关系，即h=

Glia; gliogenesis; glioma; glial cells; central nervous system; CNS; glial progenitor cells; glial fibrillary acidic protein; ependymocytes; ependoma; cytokeratins; vimentin; tanycytes; ion transport; lining cells; cerebrospinal fluid; CSF; ventricular system; floor plate; axon guidance; choroidal cells; choroid plexus epithelium; Chiari malformations; congenital aqueductal stenosis; neuroblast migration disorders; gap junctions

神经胶质；gliogenesis；神经胶质瘤；胶质细胞；中枢神经系统；CNS；神经胶质祖细胞；胶质原纤维酸性蛋白；室管膜细胞；ependoma；细胞角蛋白；波形蛋白；tanycytes；离子转运；衬细胞；脑脊髓液；CSF；脑室系统；底板；；脉络膜细胞；脉络丛上皮；恰里畸形；先天性导水管狭窄；成神经细胞迁徙扰乱；缺隙连接点

For the veneering with 0.3mm thick veneer over a graduated particleboard, the optimum processing conditions were a UF adhesive level at 78g/m~2 over the board, a PVAC adhesive level at 42g/m~2 over the veneers and 4.6 percent 36％ HCL ...

用0.3mm厚的水曲柳薄木对渐变结构刨花板饰面时，其最佳工艺条件为：脲醛树脂胶在刨花板上的涂胶量为78g/m~2，聚醋酸乙烯酯乳液在薄木上的涂胶量为42g/m~2，38％盐酸用量为聚醋酸乙烯酯乳液重量的4.6％。

Collagenase activity was defined as the radioactivity extractable by 50% dioxane. Clostridial collagenase was used as a standard. The lower limit of this assay method was 0.01 units of clostridial collagenase. Among 17 glaucoma eyes, 6 had trace collagenase activity (around 0.01 units) in aqueous humor; and for cataract eyes, 8 out of 27 had trace collagenase acitivty.

我们所采用之测量方法可侦测出约0.01单位以上的胶原酵素；一般前房液之胶原酵含量非常低，许多患者无法以我们之方法利得胶原酵素之存在：17位青光眼患者中，只有6位可於其前房液中到得约0.01单往之胶原酵素；27位白内障急者中，有8位可测得微量胶原酵素；二者在统计学上与太大差异存在。

Results (1) the laminin synthesis was significantly improved by PBMC from nontreatment group (P＜0.05);(2) the 3 H-prolin incorporation was significantl decrease in the GEC treated by PBMC supernatant from nontreatment group(P＜0.05);(3) the supernatant of PBMC from nontreatment group stimulated the collagen Ⅲ synthesis(P＜0.05), while, there was no significant difference of collagen Ⅳ synthesis between 4 groups;(4) the activity of PBMC supernatant have no close relation with urinary protein excretion, pathological type and clinical type.

结果 (1)肾病极期未经激素治疗组PBMC上清明显促进了GEC合成层粘连蛋白；(2)未治PBMC上清抑制了GEC合成总胶原；(3)未治组PBMC上清促进了Ⅲ型前胶原的合成，而对Ⅳ型胶原的合成无明显影响；(4)肾病患儿PBMC的上述作用与是否足量激素治疗有关，而与尿蛋白能否阴转、肾组织病理类型、肾病临床类型等无直线相关关系。

About 48%(90/194) were below 29 years of age. Ninety-nine of 194 cases had either radiotherapy alone or supplementary treatment with radiotherapy following surgery. We analyzed multiple prognostic factors. The factors of importance included postoperative radiotherapy; female in glioblastoma multiforme; the primary tumor dose above 5000 rads and/or whole brain irradiation in malignant astrocytoma and glioblastoma multiforme; the primary tumor dose above 4500 rads in ependymoma, medulloblastoma and oligodendroglioma; and spinal axis irradiation above 2000 rads in ependymoma and medulloblastoma that produced a better prognosis than in other groups.

男与女之比例为2.7比1，年龄分布自1至81岁，平均年龄33.3±19.3岁，中值年龄为34岁，29岁以下占48%(90/194)，其中99例单独或手术后辅以放射腺治疗，分析同种脑瘤的预后因素，其中多形神经胶母细胞瘤有手术后放射线治疗或女性，多形神经胶母细胞瘤合并星细胞瘤原发肿瘤剂量5000雷得以上或曾全颅腔照射，神经管胚细胞瘤，间胶质母细胞瘤，及室管膜瘤原发肿瘤剂量4500雷得以上，室管膜瘤及神经管胚细胞瘤有脊轴照射2000雷得以上预后均佳，且统计上有显著的差异。

The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.

结果表明，处理后4周，全周角膜缘上皮板层手术切除，中央角膜上皮层用1 mol/L NaOH擦除的5只试验家兔角膜表面全部血管化、结膜化，未发生睑球粘连，角膜基质胶原纤维完整未见溃疡、穿孔等病变，细胞印迹学检查为结膜表型，可作为实验性角膜缘干细胞移植的病理模型；全周角膜缘上皮板层手术切除，中央角膜上皮用生理盐水擦除的5只试验家兔，有2只为结膜表型，另3只为角膜表型，观察期内结果不稳定；半周角膜缘上皮板层手术切除，中央角膜上皮层用生理盐水擦除的5只试验家兔，角膜表面透明，全部为角膜表型；直接用1 mol/L NaOH擦除角膜缘和中央角膜上皮的试验家兔，有4只角膜基质胶原纤维断裂、溶解，并伴有严重的溃疡、穿孔、睑球粘连等病变，不能用于移植试验，另1只角膜表面透明，未见结膜和新生血管长入，细胞印迹学检查为角膜表型。

And Pharaoh spoke to Joseph, saying, Your father and your brothers have come to you.

47：5 法老对约瑟说，你父亲和你弟兄们到你这里来了。

Additionally, the approximate flattening of surface strip using lines linking midpoints on perpendicular lines between geodesic curves and the unconditional extreme value method are discussed.

提出了用测地线方程、曲面上两点间短程线来计算膜结构曲面测地线的方法，同时，采用测地线间垂线的中点连线和用无约束极值法进行空间条状曲面近似展开的分析。