上胶

Purified protein samples were separated by 2-DE with 17 cm IPG strips, and the protein spots were detected by fast silver staining. Six protein spots were randomly excised from the silver stained gel for primary analysis by MALDI-TOF MS. Four proteins were identified, and they were 1-aminocyclopropane-1-carboxylate synthase 2, ribulose-1, 5-bisphosphate carboxylase/oxygenase large subunit, F11O4.2 and Rubisco small subunit.

根据预实验结果，选用17 cm IPG预制干胶条对纯化的蛋白样品进行2-DE实验，采用快速银染法进行染色，随机挖取银染胶上6个蛋白点进行MALDI-TOF MS分析和数据库检索，鉴定了3个蛋白，它们是：1-氨基环丙烷-1羧酸合酶2，核酮糖-1，5-二磷酸羧化酶/加氧酶大亚基和F11O4.2。

Mainly using protein - film voltammetric technique, we have achieved direct electrochemical response of lots of proteins such as hemoglobin, myoglobin, cytocrome c, cytocrome b5, horseradish peroxiase, GOD ,Vitreoscillia sp. haemoglobin, etc., at a series of protein membrane modified electrodes, for instance, protein/kieselgubr, protein/polyethylene glycol, protein/polyethyleneimine, protein/phosphatidylcholine, protein/DNA, protein/montmorillonite/polyvinyl alcohol, protein/silver nanoparticle/ cysteamine membrane modified electrode, etc., which are prepared by casting, incorperating or layer by layer self-assembly methods. Electrochemical behavior of these proteins in the films have been studied, and their structural and conformational changes have also been examined with spectrometric techniques.

三年来，在基金委的支持下，我们以蛋白膜伏安法这一新技术作为主要的研究手段，并结合其他生物、化学技术，研究了血红蛋白、肌红蛋白、细胞色素C、细胞色素b5、辣根过氧化物酶、葡萄糖氧化酶以及好氧革兰式阴性菌透明颤菌中的血红素类蛋白等多种蛋白质在以涂布和自组装方法制成的蛋白质/硅藻土膜、蛋白质/聚乙二醇膜、蛋白质/卵磷脂膜、蛋白质/聚乙烯亚胺膜、蛋白质/蒙托石/聚乙烯醇自组装膜以及蛋白质/银胶/半胱胺自组装膜等修饰电极上的直接电化学行为，结合各种光谱技术，对蛋白质在膜中的构象进行表征，探索其电子传递机理。

ShTat1.3 and ShTat1.5 are two variable antigen types of a cloning Anhui strain of Trypanosoma evansi.Total RNA of the two variable antigen types were extracted by acid guanidinium thiocyanate-phenol-chlorform single step method.

应用异硫氰酸胍一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1.3和ShTat1.5总RNA，在含有甲醛的琼脂糖凝胶电泳后，转印到硝酸纤维膜上。

It still has a single-action lever in the tailgate for easy opening and closing, there are rope hooks on the outer edge or the insides of the cargo-box walls, and there are load rest stoppers at the rear of the cabin that can be raised to secure items too long for the cargo bed.

它仍然有一个单一的，易于开启和关闭尾门行动的杠杆，有绳钩的外缘或货物的内侧箱壁，并有负载休息胶塞的后认为可以提高，以确保对货物的床上用品太长客舱。

Wooden floor installation checks and accept a standard: Material should accord with resident requirement and active product standard; The floor is installed should firm, breathed noise, without become loose; Wooden floor surface should plane burnishs, wait for a phenomenon without broken bits of dig mark, dig and burr, the floor of besmear varnish its design should clear; Juncture of floor spelling a flower is answered neat, aperture width is consistent, the surface is clean, without excessive glue phenomenon; Skirting board and floor connection are close; The edge on skirting board is flat, differ high endlong should not be more than ± 3 millimeter and metope are clingy, without aperture.

木制地板安装验收标准：材料应符合住户要求及现行产品标准；地板安装应牢固、无声响，无松动；木地板表面应刨平磨光，无刨痕、刨渣和毛刺等现象，涂清漆的地板其图案应清晰；拼花地板接缝应对齐，缝隙宽度一致，表面洁净，无溢胶现象；踢脚板与地板连接紧密；踢脚板上沿平直，全长高差应不大于±3毫米与墙面紧贴，无缝隙。

On top of this slather your hands with your favorite hand lotion.

可以在内鞋底上喷点发胶，这样脚在里面就不会打滑了。

Sol-gel technique was used to prepare thin films of CdS microcrystallite doped SiO 2/TiO 2 glass onto soda lime glass substrates.

采用溶胶-凝胶方法在普通的载玻片上制备了CdS微晶掺杂的TiO2/SiO2复合薄膜。

Firstly, based on the review of the current researches and development in Dye-Sensitized nanocrystalline Sollar Cells, the anatase nano-porous TiO_2 powder is preparated by Sol-Gel method.

首选，本论文在总结染料敏化纳米晶太阳能电池工作原理、效率的提高和最新发展的基础上，用溶胶-凝胶法制备了锐钛矿晶型的纳米晶多孔TiO_2粉体。

As Spiker romps through the grass with Marcy after his lesson, it's obvious that the two have bonded.

下课后，玛希与斯派克在草地上嬉闹玩耍，很明显他们现在已经如漆如胶，难以分开。

And we put clear gelatin over them, just like they make them in Starburst?

然后在上面放上透明凝胶，就像他们在 Starburst 水果糖中放的一样？

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！