上胶

Here, by introducing C-terminally 6xHis-tagged human Nap1 gene into Escherichia coli and exploiting the nickel-charged sepharose gel system to purify the human Nap1 protein, we show that the Nap1 enzyme function is preserved in human as in lower organisms such as bacteria and plants using succinamic acid as the substrate.

此研究中，我们将已在胺基末端接上6个组胺酸标记的Nap1基因表现在大肠杆菌中，并使用接合上镍原子的琼脂糖凝胶纯化出人类Nap1蛋白，再以丁醯胺酸作为受质，证明人类Nap1蛋白具有酵素活性。

We prepared critical size segmental bone defects in rat tibiae and transplanted GAM consisting of boine atelocollagen and expression plasmid ector (bmp2), which encodes human BMP2, with or without CaP.

我们在大鼠胫骨上做了临界大小的节段性骨缺损，并移植上包含牛次发育胶原、表达bmp2质粒的GAM，后者编码人BMP2，不管有没有磷酸钙沉淀物的存在。

Some spore-pollen assemblages of late Trias and the youngest zircon of 225Ma were also obtained in this type.Deriveration of the ore is from the upper crust according to the features of trace elements,zircorn U-Pb age and initial value of ~(87)Sr/~(86)Sr.It has not any charateristics of the mantle.

底辟-侵位角砾岩是本次工作新发现的，它在空间上与构造-膏溶角砾岩密切伴生，剖面上呈脉状或火焰状分布，局部呈脉状穿刺于围岩中，其胶结物中含有晚三叠世的孢粉组合，最年青锆石的年龄为225Ma，角砾岩本身是铅和锌的富矿体。

Objective: To explore the effects of uremic serum on proliferation and collagen secretion of human renal tubular epithelial cells.

目的探讨尿毒血清对人肾上管上皮细胞侏(HK-2)增殖和胶原蛋白分泌的影响。

They are capable of surviving out of water, and when on land their skin secretes a gelatinous goo to retain moisture However, their large paddle feet are better suited for swimming, and waterhorse dragons tend to move very awkwardly when on land.

它们不在水里也能存活，当在陆地上时它们的皮肤会分泌一种凝胶状的粘液以保持湿润。然而它们大桨一般的脚更适合用来游泳，当在陆地上时水龙马移动得相当笨拙。

Electron microscopic findings were: 1. alveolar type I cells were degenerated、 broken-down and desquamated, endothelial cells were swelled, with inter cellular tight junction shortened, alveolar type II cells hyperplastic, basement membrane thinned and deformed; 2. alveolar macrophages and interstitial macrophages were hyperplastic; 3.mast cells were infiltrated and degranulated; 4.electron-dense deposits were present at alveolar wall; 5. myofibroblasts 、fibroblasts、 collagen and basement membrane like material were hyperplastic.

电镜观察可见：(1)I型肺泡上皮细胞变性、崩解和脱落，内皮细胞肿胀，细胞间紧密连接短小，II型肺泡上皮细胞增生，基底膜变薄和破坏；(2)肺泡巨噬细胞、间质巨噬细胞增多；(3)肥大细胞浸润并见脱颗粒现象；(4)肺泡壁电子致密物沉积；(5)肌纤维母细胞、纤维母细胞、胶原原纤维及基底膜样物质增生。

The recombinant viruses, with the gene of reference RV strain VP2, field strain T114 VP6 and T73 G1-serotype VP7, respectively, co-infected Sf9 cell at a multiplicity of infection of 0.5. Harvested the supernatants of culture when the cells were lysed fully (about 7-10 days), or harvested cells before cell lysates (about 4-5 days after infection) and then treated the cells with lysing solution. The supernatants of and cell lysates were ultracentrifuged twice with 40% sucrose cushion at 93 000g, and the sediment products were harvested with TNC buffer. The purified VLPs samples were separated by SDS-PAGE, and then stained by Coomassie blue and silver nitrate and detected by Western blot to analyze the composition of VP2, VP6 and VP7 and their specificity. The morphologic structure of recombinant VLPs was detected by EM.

含标准株VP2、地方株T114 VP6和地方株T73 G1型VP7蛋白编码基因的重组病毒以0.5 MOI共感染Sf9细胞制备2／6／7病毒样颗粒，待细胞完全裂解后收获培养基上清或感染后5天细胞裂解前收获细胞并用裂解液裂解，培养基上清和细胞裂解液样品两次40％蔗糖垫93000g超速离心，沉淀样品经SDS-PAGE胶分离，用考马氏亮兰和硝酸银染色以及Western blot检测样品中VP2、VP6和VP7蛋白的组成以及特异性，电镜检测重组病毒样颗粒的形态结构。

Screws through the hole at the corner of the Box holder and 25mm.

3按照下面螺丝位置图的指示，将两粒3x40mm螺丝装入主机板保护套上，并分别套上25mm及3mm胶制丝母。

Since the deprivation of quality bloodstone, some people get high profits, often filled with artificial color added (red pigment or cinnabar powder mixed with 502 plastic, hand-painted color), or collage blood stone (cut into thin slices to high-quality bloodstone affixed large stone on the poor) and other methods to false.

由于优质鸡血石的匮缺，一些人为获取高额利润，常采用人工填加血色（用红色颜料或辰砂粉拌以502胶，手绘上色），或拼贴血石（把优质鸡血石切成薄片贴于大块劣质石材上）等方法来作假。

Different types of clothing of the composite material made of fabric, or suitable for clothing, sportswear, big jacket, jackets, shoe materials, gloves, brassieres, handbags, hats, furniture, sofa, toys, cushions, curtains , Baby carriages, noise and other materials 6, tpu affixed with waterproof breathable waterproof membrane film has a unique waterproof materials, windbreak, permeability, soft, washable the movement of sweat can be generated by steam quickly from in vitro, Dress is hot and will not be able to regulate body temperature appropriate to maintain the body dry, complex breathable waterproof membrane applied to sportswear, big jacket, windbreaker, raincoats, Fang Hanfu, gloves, caps, military special clothing, shoe materials, affixed with waterproof Film can be applied to waterproof shoes, sport shoes, Air Shoes, sports Hudian, pu foam, medical mattresses, sleeping bags, and so on.

成衣复合不同种类的材料复合后做成的面料或内里适合于成衣，运动服，大褛，夹克，鞋材，手套，胸围，手袋，帽，家私，沙发，玩具，座垫，窗帘，婴儿车，隔音材料等 6、tpu防水膜贴上防水透气薄膜的材料具有独特防水，防风，透湿，柔软，耐水洗的特性能将运动时流汗所产生的水蒸气迅速排出体外，使穿着时不会感到闷热并且能适当调节体温，保持身体干爽，复合防水透气膜适用于运动服，大褛，风衣，雨衣，防寒服，手套，帽，军用特种服，鞋材等，贴上防水胶膜可适用于防水鞋，运动鞋，气垫鞋材，运动护垫，pu发泡，医疗用床垫，睡袋等。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！