上胶

" The one hint of the future to be found among still familiar older senses of the word, such as "a thin skin or membranous coating" or "an abnormal thin coating on the cornea, is the sense of film used in photography, a sense referring to a coating of material, such as gelatin, that could substitute for a photographic plate or be used on a plate or on photographic paper.

在当中发现的对未来的提示仍然同这个词的旧有意思相近，例如"一层薄的皮或覆盖的薄膜"或"角膜上一种不正常的薄的覆盖物"，就是film 用于摄影的含义，意思指覆盖物，如胶，可以代替感光板或用于感光板上或在相纸之上。

Or .an abnormal thin coating on the cornea,.is the sense of film used in photography, a sense referring to a coating of material, such as gelatin,that could substitute for a photographic plate or be used on a plate or on photographic paper.

或。角膜上一种不正常的薄的覆盖物。，就是 film 用于摄影的含义，意思指覆盖物，如胶，可以代替感光板或用于感光板上或在相纸之上。

Go up by the surface in light of, it seems that the two sides of the Taiwan Straits restores a dialog, still ankylose in " 92 consensus " on this conflict dot.

由表面上来看，似乎海峡两岸恢复对话，仍胶着在"九二共识"这个争执点上。

Based on the cloning FeSOD gene of the HZNH, the recombinant prokaryotic expression vector, PQESOa/FeSOD, was constructed and digested with restriction endonuclease BamH I and Pst I to check its construction. The PQE30a/FeSOD was then transformed into E.coli Ml5 and induced with IPTG. The high expression in vitro was obtained and analyzed on SDS-PAGE gel. The*results showed that the target proteins held a 37% portion in whole bacterial proteins and consisted of two parts, the soluble proteins and inclusion bodies. The soluble proteins in the aqueous layer, checked by means of activities of FeSOD enzymes and analyzed by means of activities of isozymogram from PAGE, demonstrated the induced expression proteins had the active nature of FeSOD enzymes.

以克隆的特异种质烟草HZNH的FeSOD基因为基础，构建了原核表达载体PQE30a／FeSOD，经限制性内切酶BamH Ⅰ、Pst Ⅰ双酶切鉴定后，再转化入大肠杆菌M15中，通过IPTG诱导，得到高效体外表达，经SDS-聚丙烯酰胺凝胶电泳检测，表达的目的蛋白占总菌体蛋白的37％，可溶性和包涵体两种形式均有存在，上清中的可溶性蛋白经FeSOD酶活测定和同工酶活性谱带分析，表明诱导表达的上清中的目的蛋白为有活性的FeSOD酶。

Methods KC and FE of tympanic membrane were cultured on the surface of CCM prepared by torrefaction, then the cells biological characteristics were detected by immunofluorescence stain of keratin and vimentin, and the cells proliferative activity were identified by immunofluorescence stain of PCNA.

方法热干法制备CCM，传代的大鼠鼓膜上皮KC和FB分别接种于培养板及CCM表面，通过角蛋白、细胞膜波形蛋白免疫荧光染色鉴定细胞特异性，通过细胞核增殖抗原(proliferating Cell nuclear antigen， PCNA)免疫荧光染色测定膜上细胞的增殖能力，通过细胞培养液上清的经脯氨酸测定评价对FB胶原分泌功能的影响。

Biofilm is fragmented and mainly composed of zoogloeas on the cracks and concaves of the filter media. The bacteria in biofilm or activated sludge of aerobic filter are mainly bacilus and filamentous bacteria.

滤料上的生物膜是不连续的，微生物主要以菌胶团的形式存在于滤料上的孔洞内和表面凹陷处；无论是滤料上的生物膜，还是滤床孔隙内的活性污泥，其中的细菌以各种杆菌及丝状菌为主。

The present invention has stepped motor and sync belt driver to drive the label roll, guide wheel and magnetic steel plate to separate the upper paper layer and the lower paper layer from the label belt, electromagnet driven cutter to cut the adhesive film between two successive labels, electromagnet driven magnetic steel block to transfer the label onto the label inserting board, and elevating and rotating manipulator to insert and stick the label inserting board into book seam.

本发明通过步进电机、同步带传动驱动卷标上下层纸带收卷轮的转动，上层纸带与标签及下层纸带与标签之间的分离通过一导轮和磁钢板实现，连续型卷标相邻两个标签之间的不干胶膜由电磁铁驱动的切刀切断，金属标签通过电磁铁驱动磁钢块撞击取签插板而使标签脱离下层纸带被转贴到取签插板上，取签插板手动插入或取出，或者用升降旋转机械手将末端贴有标签的取签插板插入翻开的图书中间夹缝内，使标签转贴到图书内纸上。

Drawing on: The process of attaching a paper or board cover to a book by means of a strip of glue at the spine of a book block.

上书皮：在书芯的脊上加胶，再把纸或咭纸书皮黏上的操作。

The VVA0326- mutant carrying a deletion of the GGDEF domain displayed smaller colony morphology on LB plate while compared to the wild type. No difference in colony forming units was found indicating that growth rate is not likely a major factor to confer the phenotype of small colony. In addition, VVA0326- appeared to swim better than wild type bacteria on the swimming plate. VVA0326- grew better, while VVA0328- mutant, which carried a deletion of the EAL domain, grew worse than wild type when the bacteria were cultured on CAS plate at 30°C. This implied that changes in surface structures of the mutants affected the bacterial susceptibilities to HDTMA contained in CAS plate.

缺损了包含一个GGDEF功能区域的突变株 VVA0326-，培养在LB的洋菜胶上，呈现出较小的生长菌落，在测定单位菌落数的结果发现，VVA0326-在生长速度上，相较於野生株并没有不同，因此我们推论生长速率并不是造成菌落变小的主要因子，除此之外，VVA0326-在游泳的分析培养皿中游的比野生株快，在CAS的分析培养皿上，与野生株比较，VVA0326-有比较好的生长状态，而缺损了包含一个EAL功能区域的突变株VVA0328-，却有被抑制生长的现象，可能是因为突变株影响了表面的结构，进而改变了细菌对於HDTMA的敏感层度。

Methods: One-step trizol had used to get the total RNA of the four animal brain, then PEGS000 to purify the small RNA from the total RNA, after the ligation ofpoly on the 3\' and the adaptor on the 5\', the oligo primer will be used to reverse translation the small RNA, and then used the specific primers to amplification the small RNA sequences. After the gel purition recovery, the small RNA will be ligated to the T-vector, and be transformed to the TOP 10 E. coli, at last, we picked up the masc. clones for sequencing.

通过Trizol一步法提取四种动物脑中的总RNA，接着用PEG纯化其中的小分子RNA，再经过poly连尾反应在小RNA的3'端连接上一段poly，然后在5'端连上adaptor，用Oligoprimer将连接好的小RNA进行反转录，用根据adaptor和Oligoprimer设计的引物来扩增小RNA，扩增后的小RNA进行胶回收后连接到T载体上，转化TOP10大肠杆菌后挑取阳性克隆进行测序，得到的序列再经过生物信息学的分析后确定是否为miRNA。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！