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We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此,本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs,使用无血清培养基进行扩增、培养,用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定,对少突胶质系细胞表达部分营养因子的情况进行检测;采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测;将OPCs移植入成年SD大鼠玻璃体内,利用上丘逆行荧光标记技术,观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间;将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内,观察不同时期视网膜内髓鞘形成与分布特点,分析髓鞘的超微结构,并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

In addition, the nozzle structure is complex and there are some parts of the factory of the nozzle is sealed with adhesive, glue is organic, most of the glue is impatient keto esters and organic solvents, alcohols, of course, including the nozzles on the sealant.

另外,喷头的结构很复杂,出厂的喷头有一些部位是用胶粘剂密封的,胶是有机物,大部分的胶是不耐酮酯醇类有机溶剂的,当然也包括喷头上的密封胶。

In group A,6 weeks postoperatively, a great deal of neogenetic cartilage was observed and in 12 weeks postoperatively, the shape and maturity of neogenetic cartilage were observed close to normal level in group A, which had advantages over those in group B、C.

结果Ⅱ型胶原海绵与Ⅱ型胶原膜在材料特性上有显著差异,胶原自发荧光分层扫描通过图像分析得出A组平均孔径为(93.26±38.41)μm,弹性模量(0.098±0.0 017) MP/m2,均较B组更为理想。

At histopathologic analysis, neuronal tumors are usually classified as pure neuronal cell tumors (gangliocytoma, Lhermitte-Duclos disease , central neurocytoma) and mixed neuronal-glial tumors (ganglioglioma, desmoplastic infantile ganglioglioma, dysembryoplastic neuroepithelial tumor, ganglioneuroma).

根据组织病理学的分析,神经元肿瘤通常被分为:单纯的神经元细胞肿瘤(神经节细胞瘤,Lhermitte-Duclos 病〔发育不良性小脑神经节细胞〕瘤),中枢神经细胞瘤)和混合性的神经元-胶质肿瘤(神经节神经胶质瘤,成结缔组织性婴儿节细胞胶质瘤,胚胎发育不良性神经上皮瘤,神经节瘤)。

Results: The findings on CT and MRI were as follows: 57 cases of Oligodendroglioma, 15 cases of Ganglioglioma , 5 cases of Dysembryoplastic neuroepithelial tumor , 3 cases of Pleomorphic xanthoastrocytoma .

结果:少枝胶质瘤47例,间变性少枝胶质瘤10例,节细胞胶质瘤15例,胚胎发育不良性神经上皮瘤5例,多形黄色细胞瘤3例。

02 Sandwich rubber is a single layer of cellular rubber covered with a single outer layer of ordinary pimpled rubber, the thickness of the pimpled rubber not being more than 2mm.

海绵胶是在一块泡沫胶上加盖一层颗粒胶,该颗粒的厚度不得超过2公厘。

Sandwich rubber is a single layer of cellular rubber covered with a single outer layer of ordinary pimpled rubber, the thickness of the pimpled rubber not benig more than 2 mm .

&海绵胶&即在一层泡沫橡胶上覆盖一层普通颗粒胶,普通颗粒胶的厚度不超过2毫米。

Secondly, according to the construction and characteristic of the N-isopropylacrylamide and the temperature-sensitivity of PNIPA hydrogel, Pdualistic hydrogel was synthesized by microwave low temperature plasma. The effects of the factors on the swelling characteristic of P dualistic hydrogel, such as treatment time and power of plasma, concentrations of crosslinking agent and total monomers, proportion of AMPS, polymerizing temperature etc, was discussed in detail. The three dimensions structure , thermostability , reversional volume , swelling/deswelling kinetics, temperature-sensitivity and pH-sensitivity of the hydrogel were also investigated. The optimal synthesizing technics of P dualistic hydrogel initiated by microwave low temperature plasma was confirmed.

在此基础上,结合NIPA的结构特征和PNIPA的温敏性,用微波低温等离子体引发聚合了P二元智能凝胶,详细探讨了微波低温等离子体处理功率、处理时间、总单体浓度、AMPS单体浓度、交联剂浓度及聚合反应溫度等因素对P二元凝胶溶胀性能的影响;并对其三维交联结构、热稳定性、失水-吸水动力学、温度敏感性、pH敏感性、体积复原性等进行了研究;确定了适合微波低温等离子体引发聚合P二元智能凝胶的最佳工艺。

Reconstruct ion of interstitial collagen in sinuatrial node occur since the rat diabetes has been duplicated for 16 weeks. The unbalance between collagen type Ⅰ and type Ⅲ in sinuatrial node of diabetes rat can be reversed by insulin treatment.

Ⅱ型糖尿病大鼠窦房结16周后出现间质胶原重构,胶原类型比例失衡;Ⅱ型糖尿病大鼠行胰岛素降低血糖后可不同程度下调窦房结间质的沉积,一定程度上逆转胶原类型比例紊乱。

The material is 48H,50M,39UH,35EH to be produced in batch .we can produce magnet 80 tons per month at present .the products is DISC magnet of motor ,cup magnet of vacancy ,VCM magnet ,speaker magnet in mobile ,Nuclear magnetic resonance etc ,flexbile magnet have magnet piece/mount PVC/two sides glue、anisotropic plastic magnet、plastic soft magnet、magnet strip/mount tow sides glue、magnetise printed paper etc,product to be can printed or pvc on no-magnetic side directly、mount tow sides gluethis product can processed according to custom,for example

主要常见产品有扁平电机磁钢,空心杯磁钢,UCM磁钢,手机受话器磁钢,核磁共振磁钢等等各类电子产品零配件;橡塑磁有磁片/裱pvc/双面胶、异性橡塑磁、橡塑软磁片、挤出磁条/裱双面胶、磁性打印纸等,产品可直接在无磁面上印刷或在裱在无磁面上的pvc上印刷、无磁面裱双面胶(这种产品客户可自己加工,比如贴彩纸),另外一面是充磁面,它直接可粘在带铁的东西上面(比如汽车、冰箱、或展架等),使用起来比较方便、简单。

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In the United States, chronic alcoholism and hepatitis C are the most common ones.

在美国,慢性酒精中毒,肝炎是最常见的。

If you have any questions, you can contact me anytime.

如果有任何问题,你可以随时联系我。

Very pretty, but the airport looks more fascinating The other party wisecracked.

很漂亮,不过停机坪更迷人。那人俏皮地答道。