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The ligamentous scaffold material were made from the PVA fibers by braiding technique. The mechanical properties of the scaffold materials were measured using the electronic materials testing machine. Human ACL cells were isolated and cultured by tissue explant and collagenase digestion methods, the morphous characteristics of cells and the secretion of collagen protein were measured. In order to observe the biocompatibility between the material and cells, ACL cells were in vitro expanded and seeded on the scaffold materials.

方法]用聚乙烯醇纺丝纤维编织构建韧带支架材料,在电子拉力机上测试该支架材料的力学性质;用组织块和胶原酶消化培养法在体外分离培养人前交叉韧带细胞并对细胞的生长形态和分泌胶原蛋白的特征进行检测,细胞经体外扩增后种植于编织构建的聚乙烯醇韧带支架材料上观察。

Result The flexibility of the scaffold material was powerful. The load-elongation curve of the mechanical testing was similar to that of ACL, the maximum load, the ultimate stress and the Young's modulus of the scaffold materials were 52.61 N, 14.96 MPa and 202.08 MPa respectively. Human ACL cells displayed the representative characteristercs of the ligamentous fibroblasts, and synthesized extracellular matrix, such as type Ⅰand Ⅲ collagen protein. The scaffold materials had no cytotoxicity. Human ACL cells adhered, grew and proliferated well both on the surface and in the holes of the scaffold materials.

结果]韧带支架材料的柔韧性强,拉力测试的负荷-拉伸曲线与韧带的拉伸曲线相似,其最大负荷、极限应力和弹性模量分别为52.61N、14.96MPa和202.08MPa;体外分离培养的人前交叉韧带细胞呈典型的成纤维细胞特征,能在体外分泌Ⅰ、Ⅲ型胶原等细胞外基质;支架材料无细胞毒性,人前交叉韧带细胞可在支架材料上黏附、生长并分泌细胞外基质。

First, we investigate the methods of sepiolite s purification from ore , from which we get the incompact short fibrous sepiolite for the experiment ; the prime activatited term is also obtained through this experiment. We use activated sepiolite to process the absorption experiment on heavy meatal ion ( Ni2+, Cr6+, Pb2+, Cu2+, Cd2+), noble metal ion, dynamite cotnposton in water and industrial oil. we take Ni2+ for example to confirm the prime adsorbent term , from the experiment we get the adsorbent isotherm . Make the dynamic absorption experiment and regenerate experiment using sepiolite on the heavy metal(Ni2+) on the base of static absorption experiment.

我们首先研究了从矿石中提纯海泡石的方法,制得供实验用的松散短纤维状海泡石;通过实验确定了最佳活化条件;进行了活化后的海泡石对重金属离子Cr~(6+、Pb~(2+)、Cu~(2+)、Cd~(2+)、贵金属离子、水中炸药组份、工业油的吸附实验;以Ni~(2+)为例选择了最佳吸附条件;通过实验作出了以上各组分的吸附等温线;在静态吸附实验的基础上进行了海泡石对重金属Ni~(2+的动态吸附实验及再生实验。

At the mean time,the culture mixed with the fibroid cells which grew well respectively.Fine single layer was formed and covered the whole amniotic membrane on which the LECs grew membranously,and the fibroid cells outgrew the LECs.

同时,生长的上皮细胞中有成纤维样细胞的混杂生长,但各自保持其良好的细胞形态。3周左右形成良好的单层,细胞生长呈膜片状扩展,整个羊膜之上铺满细胞,有明显的扩展界限。

Among them are the indefinite shape mesenchyma.

在电镜下发现中段大直径胶原纤维明显多于上、下段(P<0.01)。

When reached logarithm growth phase, it was poured or the medium was removed. 5 mL medium containing 10 mg/L mitocin-C was added , kept at 37 ℃, and then cultured in saturated humidity warm box with the CO2 of 0.05 fraction volume for 2 or 3 hours. It was coated with 0.1% gelatin in 6-hole plate, laid for over 30 minutes, and then threw away or the medium with mitocin-C was removed, washed with phosptat buffer for 5 times so as to remove the mitocin-C. 2 mL 0.05% trypsin digestive cells were added, and it was observed under microscope. When crevice appeared, cells became round (about 2-4 minutes), digestion was stopped by adding medium of the same volume, and blew up with straws repetitively to make it into monoplast suspension. Special-used cover glass was put in the center of cell counting chamber. Cells were sucked in by glass siphon, and cell suspension flew out at bucket of up or down-sides of counting chamber, until the cover glass was filled with fluid. Living cell were inoculated at concentrations of 3×108, 5×108, 1×109 L-1 after their number counted.

选取2~5代的小鼠胚胎成纤维细胞,待其至对数生长期倒掉或吸掉培养基,加入含10 mg/L丝裂霉素C的细胞全培养液5 mL,置37 ℃,体积分数0.05的CO2饱和湿度温箱中培养二三小时,在6孔板中加入0.1%明胶包被,放置30 min以上,倒掉或吸掉含丝裂霉素C的培养基,磷酸盐缓冲液清洗5遍,尽量除去丝裂霉素C,加入2 mL 0.05%的胰蛋白酶消化细胞,镜下观察,当细胞间出现裂隙,细胞变圆时(约2~4 min),立即加入等量的全培养液终止消化,并用吸管反复吹打,使之成为单细胞悬液,在细胞计数板中央放置专用的盖玻片,用玻璃虹吸管吸取细胞,让虹吸管在盖玻片上或下侧的计数板凹槽处流出悬液,至盖玻片下被液体充满为止。

Cytology revealed 3-dimensional groups of ductal cells in a background of myoepithelial cells and stromal cells, consistent with a fibroadenoma.

对该肿块进行细针抽吸活检,细胞学显示以肌上皮细胞和基质细胞为背景的三维导管细胞结构,与纤维腺瘤结构一致。

Results Myoid/myofibroblastic differentiation occurred most commonly in fibrosarcomatous DFSP. It was recognized histologically as peripherally distributed or randomly scattered small eosinophilic nodules or short bundles, which were composed of bland spindle cells, closely resembling smooth muscle cells or myofibroblasts.

结果 肌样/肌纤维母细胞性分化多出现在纤维肉瘤型DFSP中,表现为肿瘤周边部或肿瘤内散在性分布的深嗜伊红色小结节或短条束,由梭形细胞组成,细胞多无异型性,核分裂象也罕见,形态上似平滑肌细胞或肌纤维母细胞。

Histologically, nodules of adipose and myxoid tissue were demarcated by bands of fibrous tissue.

组织学上,根据纤维组织的带状中性粒细胞区分脂肪和黏液组织结节。

Results The model showed serious edema, proteinuria, hypoproteinemia, and hyperlipidemia. Podocytes were swollen and mesangial cells developed mild hyperplasia at the end of the fourth week. The nephric tubule atrophied at the end of the eighth week accompanied with adhesion between glomeruli and Bowman's capsule. Glomeruli sclerosis of mild or medium degree was observed at the end of the twelfth week with obvious lymphocyte infiltration in the renal interstitium as well as the formation of collagen fibers.

结果 该模型表现为严重水肿、大量蛋白尿、低蛋白血症和高脂血症;病理学检查显示,4周末肾小球足细胞肿胀,系膜细胞轻度增生;8周末有球囊粘连,肾小管上皮细胞萎缩;12周末部分肾小球节段性轻、中度硬化,间质内有明显灶性淋巴细胞浸润,并有轻度纤维化。

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推荐网络例句

We have no common name for a mime of Sophron or Xenarchus and a Socratic Conversation; and we should still be without one even if the imitation in the two instances were in trimeters or elegiacs or some other kind of verse--though it is the way with people to tack on 'poet' to the name of a metre, and talk of elegiac-poets and epic-poets, thinking that they call them poets not by reason of the imitative nature of their work, but indiscriminately by reason of the metre they write in.

索夫农 、森那库斯和苏格拉底式的对话采用的模仿没有一个公共的名称;三音步诗、挽歌体或其他类型的诗的模仿也没有——人们把&诗人&这一名词和格律名称结合到一起,称之为挽歌体诗人或者史诗诗人,他们被称为诗人,似乎只是因为遵守格律写作,而非他们作品的模仿本质。

The relationship between communicative competence and grammar teaching should be that of the ends and the means.

交际能力和语法的关系应该是目标与途径的关系。

This is not paper type of business,it's people business,with such huge money involved.

这不是纸上谈兵式的交易,这是人与人的业务,而且涉及金额巨大。