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We introduced improved primary mixed glial culture and different-attachment method to isolate and purify the OPCs, the cells were proliferated in serum-free medium, flow cytometry and immunohischemistry methods were employed to estimate the purity of cultured OPCs. Their abilities of differentiation and expression of trophic factors were identified by RT-PCR and immunostaining. Several methods including TUNEL and MTT were adopted to estimate the protective effects of conditioned culture medium from oligodendrocyte lineage cells on the primary cultured cerebellar granule neurons. Intravitreal transplant of OPCs, combined with retrograde fluorescent labeling the superior colliculus and intraorbital optic nerve transection, were used to investigate the protective effects of OPCs on the axotomized RGCs in vivo. Intravitreal transplantof OPCs or NSCs on the newborn rats, and retinal transplant of OPCs on the young rats were performed, to observe the myelin formation in the retina at different stages after cellular transplantation. Optic nerve transection was carried out on some rats with myelinated retinae, to study the influence of myelination on the injuried RGCs.

为此,本研究采用改良的胶质细胞混合培养与差速贴壁方法获得大鼠OPCs,使用无血清培养基进行扩增、培养,用免疫组织化学和流式细胞技术对培养细胞的纯度进行鉴定,对少突胶质系细胞表达部分营养因子的情况进行检测;采用TUNEL、MTT等方法对少突胶质系细胞条件培养基对原代培养小脑颗粒神经元的保护作用进行检测;将OPCs移植入成年SD大鼠玻璃体内,利用上丘逆行荧光标记技术,观察眼内移植的OPCs对眶内视神经切断时的视网膜神经节细胞的保护作用及其持续时间;将OPCs或NSCs移植入新生和幼年SD大鼠玻璃体或视网膜内,观察不同时期视网膜内髓鞘形成与分布特点,分析髓鞘的超微结构,并观察眼内髓鞘形成对损伤神经节细胞的保护作用。

Results:Making quantitative analyses on the suffered extent ,segments and severness of lumbosacral radiculopathy .

在常规肌电图检查和神经传导检查的基础上,采用H反射和F波的近神经端测定。

Results we found positive labeling for both anp and nos in different types of cells including the spiral modiolar artery, stria vascularis, spiral limbus, spiral ligaments, basilar membrane, organ of corti, spiral ganglion, and macula sacculi. however, no immunoactivity was found in the reissner membrane and tectorial membrane.

结果 在耳蜗各转螺旋动脉和血管纹,螺旋缘、螺旋韧带和corti器显示双阳性染色,螺旋神经节细胞及囊斑神经上皮细胞膜及轴突nos阳性染色,胞质anp阳性染色;盖膜、前庭膜阴性染色。

The application of BP nerval network to failure diagnosis of hydraulic systems in construction machinery is studied. Through collection of the system data, derivation of eigenvectors and structure of classifiers, a learning algorithm is established on the basis of network training. Tests show that satisfactory results can be obtained by the nerval network.

将BP神经网络理论应用于工程机械液压系统故障诊断中进行研究,通过系统数据的采集、特征向量的提取和分类器的构造,在对网络进行训练的基础上,建立了学习算法,通过试验可以看出神经网络能给出的满意结果。

The obtained composite scaffold materials must have great biocompatibility, which could influence neuraxon regeneration and structure. An evaluation of permeability in varying molecules was a key factor to determine input of nutritive materials and output of metabolic waste.

不论用哪些材料,所制成的复合支架都要具有生物相容性,这在宏观和微观上都影响神经轴突再生的能力和结构;对不同大小分子的渗透性是评估营养物质进入支架和代谢废物排出的关键性能;要清楚支架在脊髓水环境中的膨胀程度,使其保持在合适的位置而不压迫再生的神经。

The obtained composite scaffold materials must have great biocompatibility, which could influence neuraxon regeneration and structure. An evaluation of permeability in varying molecules was a key factor to determine input of nutritive materials and output of metabolic waste. Expansion of scaffold should be understood under spinal cord water environment, because the expansion might press regenerated nerve.

不论用哪些材料,所制成的复合支架都要具有生物相容性,这在宏观和微观上都影响神经轴突再生的能力和结构;对不同大小分子的渗透性是评估营养物质进入支架和代谢废物排出的关键性能;要清楚支架在脊髓水环境中的膨胀程度,使其保持在合适的位置而不压迫再生的神经。

The first part's researches are of the interpattern association optical neural network model.(1) The neurodynamic stability of IPA model is studied after expatiating on its principle and superiority.

第一部分是关于图样间联想光学神经网络(Interpattern Association;简称IPA)模型的研究:(1)在阐述、发展图样间联想光学神经网络模型理论及其优越性的基础上,详细分析了IPA网络的动力学稳定性。

SSCALDH cells are capable of self-renewal and are able to generate new neurospheres and neuroepithelial stem-like cells.

SSCALDH细胞能自我更新并产生新的神经球和神经上皮干样细胞。

Objective To explore the isolation and culture conditions of neuroepithelial stem cells from neural tube of embryonic rats and to observe their differentiation characteristics in vitro.

目的探讨大鼠胚胎神经管神经上皮干细胞的分离培养条件,并观察其在体外的分化特性。

I think WHO grade I dysembryoplastic neuroepithelial tumor is the most likely diagnosis, but one would like to see isolated floating neurons in the microcysts in addition to the small oligodendrocyte-like cells and neuropil-like fibrillary matrix.

我考虑可能是WHOI级的DNT(胚胎发育障碍的神经上皮肿瘤)。但DNT中除了少突胶质细胞样的小细胞和神经毡样胶质纤维基质外,还应该见到微囊中漂浮的神经元。

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推荐网络例句

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发,分析对称连杆曲线上鲍尔点的产生条件,提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品,年生产总值3000万美元,产品价廉物美、选料上乘、质量保证,深受国内外客户的青睐

The eˉtiology of hemospermia is complicate,but almost of hemospermia are benign.

血精的原因很,以良性病变为主。