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The antibodies were purified and the purity and specificity were identified by SDS-PAGE and Western-blot,respectively.Lung tissues from five pigs with PRRS were tested with the established method,which clearly demonstrated the abundant presence of viral antigens in the cytoplasm of alveolar macrophages,and to less extent in alveolar and bronchiole epithelial cells.

SDS-PAGE和Western-blotting检测结果表明,制得的兔抗PRRSV IgG纯度较高,具有良好的特异性,可用于原位检测病猪肺组织中PRRSV抗原的分布;Envision法的检测结果显示,PRRSV抗原的阳性表达产物主要出现在肺巨噬细胞胞浆内,其次为肺泡上皮细胞和细支气管黏膜上皮细胞;通过对5份经RT-PCR检测确认的PRRS病猪肺组织进行检测,均有较高的阳性细胞表达,平均阳性细胞率为46.5%。

The results of the TEM showed the same respiration epithelium in the inside gill and the gill filament, it is flat, the cytoplast of the epithelium contained of the rough endoplasmic reticulum, vacuole and mitochondrion in rich; the blood-gas barrier is formed of the monolayer epithelium, the endothelial cell and the basilar membrane between the both, following the development the blood-gas barrier attenuate gradually.

电镜结果表明花背蟾蜍外鳃与内鳃具有相同的呼吸上皮,为扁平状,上皮细胞质中富含粗面内质网及空泡和线粒体;气血屏障由由单层上皮细胞,内皮细胞以及二者之间的基底膜构成,随着个体的发育,气血屏障逐渐变薄。

The re was no significant difference between the contents of SP and VIP of lung tissue in experiment group and those in control group.4. Ultrastructurally, after infected with IBV, lots of grume were observed on the surface of trachea tissue and the cilia of the trachea were massed.

免疫电镜观察结果显示,大多数肾小管上皮细胞胞浆内均见有SP及VIP阳性颗粒,在上皮细胞间还可见有DNES细胞;肺脏中异嗜性白细胞颗粒也常呈SP及VIP阳性反应,肺脏内也可见DNES细胞。

By in situ hybridization, 53BP2 mRNA expression is revealed in tumor cell nests of NPC paraffin sections. The strong positive particles localize in the nuclei of tumor cells; but slight positive signals are also found in tissue cells of chronic nasopharyngitis and connective tissue, such as lymphocytes and smooth muscle cells; There are also positive signals in the nuclei of hyperplastic squamous epithelial cells. However, 53BP1 mRNA expression is not found in nuclei of tumor cells, tissues of chronic nasopharyngitis and connective tissue.

原位杂交结果显示,石蜡切片的鼻咽癌癌巢内,可见53BP2的强阳性表达信号,信号呈紫色颗粒状分布于胞核,核仁清晰可见;而在相同条件下,在慢性鼻咽炎组织和癌旁上皮的结缔组织,也可见弱表达信号,如淋巴细胞和平滑肌细胞;在增生的鳞状上皮细胞的核内,可找到强阳性信号。53BP1在鼻咽癌、慢性鼻咽炎组织和癌旁上皮的结缔组织切片上,均无表达信号。

In order to evaluate the roles played by telomerase activity and apoptosis gene in malignant transformation of mammary atypical ductal hyperplasia, as well as the ultrastructure characteristic and regular pattern in the transformation, we investigated the expression of telom- erase genes and apoptosis related genes (p53 and bcl-2) and ultrastructure characteristic in precancerous lesion-mammary atypical ductal hy- perplasia, and compared with breast carcinoma and benign hyperplasia.

本文研究端粒酶基因、凋亡相关基因(p53、bcl-2)在乳腺癌前病变-乳腺导管非典型增生中的表达情况,探讨乳腺导管非典型增生上皮的超微结构特征,并与乳腺癌、乳腺导管单纯性增生进行对比,旨在研究端粒酶活性变化及凋亡相关基因在乳腺导管非典型增生上皮细胞癌变过程中的作用及意义,并研究乳腺导管非典型增生上皮细胞恶性转化过程中的形态结构特点及变化规律。

EGF was mainly located in the cytoplasm and extracellular matrix of intestinal villus cells, endothelial cells and tunica serosa epithelial cells, while EGFR chiefly distributed in the cellular membrane of these cells.

其中 EGF主要存在于小肠黏膜上皮细胞、黏膜下层的血管内皮细胞内和浆膜上皮细胞的胞浆和胞外基质中,EGFR则分布于这些细胞的细胞膜上。

This study demonstrated ①the infraglottic mucosa of the 〓 month human fetus iscandidate for culturing human laryngeal epithelial cells;②the cytokeratins of lowmolecular weight could be used as identified marker;③the basal and intermediatecells might possess the ability of bipotential differentiation and the ciliatedepithelium of the 〓 week fetal larynx might be a transitional form of thesquamous metaplasia.

抗低分子量细胞角蛋白抗体可用作细胞鉴定的标志;3.13周以后胎喉声门下粘膜为纤毛上皮,其基底细胞和中间细胞具有双向分化的潜能,在某些条件的刺激下,有可能化生为鳞状上皮细胞

The basolateral M cell surface is invaginated to form an intracellular central pocket into and out of which lymphocytes and macrophages.

M细胞是一种特化的上皮细胞,多分布于Peyer's结圆顶区的周边,FAE的顶端未见M细胞;其结构有别于相邻的肠上皮细胞,表面没有长而整齐的微绒毛,取而代之的是粗而短的微皱褶,胞质内终末网不发达,溶酶体较少,基底膜向顶部穹隆状突起,形成一&口袋&样结构,其中含有多个免疫活性细胞。

Results: The positive results of in-situ hybridization showed that the lung tissues of all cases expressed SARS-CoV RNA, and positive signals displayed in cytoplasms (purple-blue, NBP-BCIP.

原位杂交和免疫组化结果显示:支气管上皮细胞、Ⅱ型肺泡上皮细胞、血管内皮细胞、巨噬细胞、纤维母细胞及T淋巴细胞在所有SARS病例中都受到了病毒感染。

ISH-IHC double staining showed that positive signals of both ISH purple-blue NBT-BCIP and IHC (red-brown,AEC expressed in the cytoplasms.

原位杂交和免疫组化结果显示:支气管上皮细胞、Ⅱ型肺泡上皮细胞、血管内皮细胞、巨噬细胞、纤维母细胞及T淋巴细胞在所有SARS病例中都受到了病毒感染。

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