上皮细胞

By using the method of Western blot on fractions of nuclear and cytosol of primary cultured mantle cells and immunogold electron microscopy, we found out the completely different localization of these two proteins: CaM was localized both in cytoplasm and nuclear but CaLP was localized only in the cytoplasm.

通过对体外培养的珍珠贝外套膜上皮细胞核质分离后进行的Western blot分析和免疫电镜研究发现，CaM和CaLP的亚细胞定位是截然不同的：CaM分布于外套膜上皮细胞的细胞核与细胞质中，而CaLP却仅分布于外套膜上皮细胞的细胞质中。

With the help of Axioplan 2 imaging system, we quantified the change of expression of ICAM-1, MUC5AC, goblet cell and NF-κB. Analyze the results by SAS method. Results (1) Grouped by index of lung function, the expression of ICAM-1 at the level of gene and protein had increased significantly in the epithelial cells of patients with COPD, compared with group of normal lung function.

取病灶远端的正常叶段支气管组织制备石蜡切片，原位杂交检测气道上皮中ICAM-1的mRNA的表达量；免疫组化检测气道上皮中ICAM-1和MUC5AC的蛋白表达变化；AB/PAS特殊染色法检测患者气道上皮细胞中杯状细胞的含量；PCR扩增法检测原代培养的气道上皮中MUC5AC的基因表达量和NF-κB的变化。

Probably the intestinal barrier function correlates with decreasing [Ca2+] of the Intestinal epithelial cells to lessen the intestinal epithelial cells injury and decreasing the release of histaminase in the Intestinal epithelial cells.(4) Fufangdanshen mixture can enhance effects of Dachengqi herbs in treating incomplete intestinal obstruction.

4活血承气合剂肠屏障保护作用可能与抗自由基及降低小肠粘膜上皮细胞内钙离子超载，从而减轻小肠粘膜上皮细胞损伤，降低小肠粘膜上皮细胞组胺酶的释放有关。

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法：48小时后见大量上皮细胞贴壁，细胞不规则呈圆形、椭圆形、多边形，细胞体肥大透亮，细胞核位于中央，胞膜清楚，约12-14天后细胞呈镶嵌状排列，融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

The mean proportional volumes of mucous acini were more than those of serous acini except salivary glands of rodents.Conclusion: The mean proportional volume of MeCs in salivary glands associates with animal species and secretion of salivary glands.

HHF 35是近几年开始使用的识别特异性肌动蛋白（muscle-actin-specific）的单克隆抗体，作者使用它来区别肌上皮细胞和腺泡，希望通过定量测定比较肌上皮细胞的平均体积比，以了解不同动物涎腺中肌上皮细胞的发育是否存在物种间的差异及与分泌功能的关系。

Results The number of cellular apoptosis was obviously increased in multiple organs from the six patients died from SARS. The cellular apoptosis occurred predominantly in cytokeratin-positive pneumoncytes, terminal bronchiolar epithelium, CD3 + and CD8 + lymphocytes, as well as a part of CD20 + lymphocytes and CD68 + macrophages.

结果 与正常组织比较，SARS患者全身多器官组织细胞凋亡数目明显增多，尤以肺脏和免疫器官为甚，主要的凋亡细胞类型为CK阳性肺泡上皮细胞、终末支气管黏膜上皮细胞、CD3+细胞、CD8+细胞，以及少部分CD2 0 +细胞和CD6 8+巨噬细胞。

Among the 3 novel sequences, C24 sequence and C57 sequence were expressed both in cataractous group and in normal group; C24 sequence was expressed higher in cataractous group than that in the normal group, but C57 sequence had no significant difference in normal and cataractous group; C40 sequence was expressed in the cataractous lens epithelium but the expression in normal lens epithelium was not seen.

三个新序列中，C24号和C57号序列在年龄相关性白内障和正常晶状体上皮细胞中均有表达，C24号序列的表达增高；C57号序列在正常组和白内障组中的表达无差异。C40号序列在年龄相关性白内障晶状体上皮细胞中有表达，但其在正常晶状体上皮细胞中未见表达。

Results The expression of the Mahoganoid protein and its mRNA was showed by brown staining and observed in the Leydig cells, spermatogonia, primary spermatocytes, spermatids, Sertoli cells, and peritubular myoid cells of testicular tissues and principal cells, basic cells and epithelial cells of epididymal tissues.

结果1。免疫组化法检测Mahoganoid蛋白的实验结果显示的棕色阳性信号可见于各天龄段大鼠睾丸的生精细胞、支持细胞、间质细胞和管周肌样细胞以及附睾输出管的高柱状、低柱状上皮细胞和附睾管的上皮细胞、主细胞、基细胞。其中Mahoganoid蛋白主要表达于胞膜和胞浆。

Electron microscopic findings were: 1. alveolar type I cells were degenerated、 broken-down and desquamated, endothelial cells were swelled, with inter cellular tight junction shortened, alveolar type II cells hyperplastic, basement membrane thinned and deformed; 2. alveolar macrophages and interstitial macrophages were hyperplastic; 3.mast cells were infiltrated and degranulated; 4.electron-dense deposits were present at alveolar wall; 5. myofibroblasts 、fibroblasts、 collagen and basement membrane like material were hyperplastic.

电镜观察可见：(1)I型肺泡上皮细胞变性、崩解和脱落，内皮细胞肿胀，细胞间紧密连接短小，II型肺泡上皮细胞增生，基底膜变薄和破坏；(2)肺泡巨噬细胞、间质巨噬细胞增多；(3)肥大细胞浸润并见脱颗粒现象；(4)肺泡壁电子致密物沉积；(5)肌纤维母细胞、纤维母细胞、胶原原纤维及基底膜样物质增生。

Some exudation and bleedings happen in the pulmonary alveoli, some inflammatory cells infiltrate into lung tissues; in the serum AMY, ALP, TNF-α, IL-6 increases obviously high, COX-2 expression increase; The expression of NF-κB in big rat lung of SAP increase, and the nucleus is thick dyed, it explain that the NF-κ moved into the nucleus; Stimulate the inflamatory reaction.

我们的实验中见到SAP组的大鼠肺脏组织细胞腔扩大、肺泡壁断裂，有的出现肺大泡，肺泡内伴有渗出及少量非出血，肺组织可见咽细胞浸润；血清中AMY、ALP、TNF-α、IL-6明显增高；在SAP组大鼠肝细胞、肾小管上皮细胞、肺脏小气管及肺泡上皮上COX-2表达增加；NF-κB在SAP大鼠肺脏中表达增加，并且出现核浓染，说明NF-κB前移入核；启动炎症反应等一系列反应SAP大鼠受损伤的肺组织中AQP-1无论在mRNA水平上、蛋白水平上还是免疫组化染色病理学检测上都明显下降。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。