上皮细胞

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果：倒置显微镜和透射电镜观察显示，经SAHA处理的细胞增殖速度显著减慢，细胞体积增大，细胞核较小，形状较为规则，核仁数量减少、体积变小，核内常染色质增多而异染色质减少，核质比例减小，细胞质内线粒体数量增多、线粒体嵴发达，高尔基体较为典型，粗糙型内质网增多，呈现出与正常上皮细胞相似的形态变化；MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用，并有明显的剂量依赖和时间依赖关系；免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达；流式细胞仪检测结果显示，SMMC-7721细胞经SAHA处理后，G0/G1期细胞明显增加，S期细胞则明显减少，细胞被阻滞于G0/G1期；RT-PCR检测结果表明，SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加，24h后更为明显。

Result 1. CDK4, P16 were expressed in parabasal layers and the upper layer, mainly in the prickle and granular layers of cholesteatoma epithelium. Their positive rates were 68.6% and 88.6% respectively. Statistical analysis showed that there was a significant difference in positive rates between

CDK4与P16在中耳胆脂瘤上皮中均过度表达且有一定程度的正相关性，表明它们在疾病发生中是共同而非单独起作用的，从细胞周期的角度可反映胆脂瘤上皮细胞过度增殖的同时也增强负调控因子来抑制增殖，使凋亡同时加快而达到新的平

Expression of CD44V6 can be an early biological indicator of precarcinomatous epithelial cells. Gastric mucosa intestinal metaplasia can induce the expression of CD44V6, moreover, Hp infection can promote it.

CD44V6的表达可能是上皮细胞癌前病变出现的早期生物学信号，肠上皮化生细胞可能诱导CD44V6的表达，而Hp感染则有促进这种诱导表达的作用。

Immunofluorescence analysis showed that AQP1 was expressed in the apical and basolateral membranes of epithelial cells in proximal tubules, as well as in the thin descending limb of Henle and endothelial cells of descending vasa recta.

免疫荧光定位分析结果表明AQP1 在近曲小管上皮细胞顶膜和基底膜表达，也表达于髓袢降支细段和直小血管降支，肾囊泡上皮细胞未见AQP1表达。

The results indicate that the thyroid gland of grey mullet is dispersedly distributed in the region of the arteria branchialis afferens of the first to the third pair of branchial arches. The monolayer follicle epithelial cells around a follicle were squamous shaped or cuboidal shaped, indicating an asynchrony of cellular activity in a follicle. The scale of the two shapes of cells differed significantly among developmental stages.

结果表明，鲻鱼甲状腺滤泡分散地分布在第1对至第3对鳃弓的入鳃动脉之间；构成同一滤泡的单层上皮细胞呈扁平形和立方形两种形态，反映出同一滤泡中上皮细胞生理活动的不同步，且发现不同发育时期的鲻鱼这两种形态细胞的比例有显著差异。

Pathohistologic examination revealed normal glomeruli but vacuolar degeneration of tubular epithelial cells, and part of them disrupted and desquamated, and also tubular dilatation. Only mild pathological changes were seen in the intervention groups.

肾脏病理检查可见LPS组肾小球大致正常，而肾小管上皮细胞空泡变性，部分上皮细胞崩解、脱落，管腔扩张，两个UT干预组肾小管病理变化均较LPS组减轻，不同剂量组间形态差异无显著性。

Changes in brain tissue similar to those of newborn in HIE were detected after 2 hours of hypoxia and ischemia.Meanwhile,swelling of kidneys,hemorrhage below encapsules,swellin of tubular epithelial cells,necrosing and falling of a few tubular epithelial cells,and narrowing of tubular lumens were observed.The basement membranes of renal tubules remained normal ...

结果发现新生猪经2小时缺氧缺血后，在脑组织出现类似新生儿HIE病理损害的同时，肾脏出现肿胀，包膜下出血，肾小管上皮细胞肿胀，管腔变窄，少数上皮细胞坏死脱落，肾小管基膜完整，血清尿素氮及肌酐明显升高（P＜0.025及P＜0.05），表明在HIE状态下新生猪已出现肾脏的病理及功能损害，但损害程度较轻，解除缺氧72小时后，上述损害基本恢复正常。

The rat epididymal epithelial cells were cultured in vitro and the changes in flagelium activity and linear motile ability of the sperm that had been co-cultured with epithelium from corpus-cauda epididymides were observed.

目的：对体外培养的大鼠附睾上皮细胞进行动态观察与鉴定，了解体外条件下精子与附睾上皮细胞共同培养后精子活动力的变化。

Chapter 2 Effects of A.fumigatus extract on human bronchial epitheliums Object: Explore the effects and possible mechanism of AFE on human bronchial epitheliums.

第二章烟曲霉菌提取物对人支气管上皮细胞的作用目的：探讨烟曲霉菌对呼吸道上皮细胞的作用及其可能机制。

AIM: To investigate the expression of human β-defensin-2 (hBD-2) mRNA and the changes of NF-κB activity induced by lipopolysaccharide in human primary epitheliums. METHODS: The pulmonary primary epitheliums from human were stimulated with LPS and the expression of hBD-2 mRNA was detected by reverse transcription polymerase chain reaction.

目的：观察脂多糖诱导人气道上皮细胞人β防御素－2(hBD-2)的表达及核转录因子κB活性的变化，探讨NF-κB在LPS诱导人气道上皮细胞hBD-2表达中的作用。

Breath, muscle contraction of the buttocks; arch body, as far as possible to hold his head, right leg straight towards the ceiling (peg-leg knee in order to avoid muscle tension).

呼气，收缩臀部肌肉；拱起身体，尽量抬起头来，右腿伸直朝向天花板（膝微屈，以避免肌肉紧张）。

The cost of moving grain food products was unchanged from May, but year over year are up 8%.

粮食产品的运输费用与5月份相比没有变化，但却比去年同期高8％。