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上皮细胞

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In our study, we found menadione has anticancer potential on human breast adenocarcinoma MDA MB 231 by weather cell cycle arrest, anti proliferation, or apoptosis. Low concentration of menadione (5.8μM) significant inhibited growth curve on breast cancer cells (MDA MB 231) but not breast cells (MCF 10A) by performing MTT and trypan blue cell counting. RT-PCR and western blotting showed menadione increase p21 gene and protein expressions but decrease cyclin E and CDK2 protein expression resulting in cell cycle G0/G1 arrest by flow cytometry.

MTT及生长曲线的实验,发现menadione在低浓度且长时间作用情况下,对在於人类乳腺上皮细胞癌(MDA-MB 231)是有明显的抑制作用,而在正常乳腺细胞(MCF-10A)则没有影响,在分子转录上及蛋白质表现方面也发现低浓度menadione处理24时能使MDA-MB 231诱发p21基因及蛋白表现量增加及cyclin E、CDK2抑制,再经由流式细胞仪分析也发现低浓度 menadione 处理24小时能使MDA-MB 231细胞周期GO/G1比例上升,造成细胞周期停滞,进而抑制细胞生长。

The epithelial openings were observed on and among epithelial cells facing the subarachnoid space.The subepithelial connective tissue penetrated the pineal body forming interlobular septum,and separated the pineal body to parenchymal lobules.

在面对蛛网膜下隙的囊上皮细胞和细胞间存在许多上皮孔;上皮下结缔组织伸入松果体形成小叶间隔,将松果体分隔为许多实质小叶。

In small intestine, AKP activity was mainly distributed over four areas of epithelial cells: cytomembrane, cytoplasm, microvillus and the carbohydrate-riched cell coat. In liver, it was mainly located in canaliculi. In kidney, it mainly existed in such zones as brush border and memebrane of epithelial cells of proximal tubule, and cracks between kinds of tubules.

AKP活性在小肠主要分布在小肠绒毛上皮细胞的细胞膜、细胞浆、微绒毛和微绒毛表面的糖衣上,在肝主要分布在胆小管,在肾主要分布在皮质肾小管,尤以近曲小管的刷状缘、近曲小管上皮细胞膜和各种管状结构之间的腔隙内分布较多。

Results In animal study, lumbricus could inhibit the growth of staphylococci, bacillus coli and bacillus aeruginosus. The time of wound healing in experimental group was 4 days shorter than that in control group. At 4d and 7 day the numbers of the capillary, blood vessel endodermis and desmohemoblast desmocyte and splitting epithelium of trial group were much more than those of control group. At 4d the trial group′s numbers of splitting mesenchymal cell were much more than that of control group. From 3d on the wound healing and granulation filling of experimental group were much quicker than those of control group.

结果 动物实验中,地龙能抑制金黄色葡萄球菌、大肠杆菌及绿脓杆菌的生长;实验组创面愈合时间较对照组提前了4天;第4、7天实验组动物创面的毛细血管数、血管腔内皮细胞数及间质成纤维细胞数均较对照组明显增多,上皮细胞分裂象也高于对照组,第4天的间皮细胞分裂象也高于对照组;自第3天始创面愈合及肉芽充填速度实验组明显快于对照组。

In order to analyze experimentally the back-scattering micro-spectrum difference between human normal stomach epithetic cell and the cancerous cell to provide optical technique for clinical checking,a micro-spectral measurement setup for checking cell was established based on fiber confocal microscopically imaging technique combined with cell light scattering theory. It could provide micro-imaging and micro-medical spectral information simultaneously.

为了在实验上分析人正常胃上皮细胞与癌变细胞的显微后向散射光谱的区别,为临床医学检测提供实验依据,在光纤共聚焦显微成像技术基础上,结合细胞散射理论,建立了一套基于光纤共聚焦的细胞检测显微光谱分析装置,能够同时获取细胞显微图像和显微光谱医学信息。

There were 8 patients in the macromastia group and 5 patients in the control group. The result didn't show significant difference between the two groups. There is no difference between the two groups in mammary epithelial cell size measurement and Akt-1/2/3 immunohistochemistry stain. The levels of Ki-67, phospho-Akt-1/2/3, and STAT5a of juvenile macromastic patients are higher than those of the patients of the control group, and the results are statistically significant.

三年内共搜集到八位巨乳症病患及五位对照组,两组以Wilcoxan rank sum test比较,乳腺上皮细胞的尺寸及Akt-1/2/3免疫组织化学染色方面在统计学上的没有显著的差异,而乳腺上皮细胞之Ki-67、phospho-Akt-1/2/3、及STAT5a免疫组织化学染色方面,则均为青春期巨乳症患者组较高,统计学上均有显著性的差异。

Methods: Prepare and identification of AFE. To detect the effects of AFE on trans-epithelium electric resistance, Tight Junction proteins occludin and ZO-1, cell wound closure ability in vitro and the production of Muc5ac and GM-CSF by human bronchi epitheliums (16HBE-14o).

制备并鉴定烟曲霉菌提取物,观察AFE体外培养人支气管上皮细胞(16HBE-14o)的跨上皮细胞电阻、紧密连接结构蛋白occludin和ZO-1、细胞体外修复功能、细胞合成分泌Muc5ac和GM-CSF的作用。

The results suggest that bFGFs bound to its high affinity receptors play an important role in lens epithelial cell proliferation and capsular opacities after cataract surgery.

结果表明,晶体上皮细胞存在bFGF受体,当bFGF与其高亲和性受体结合后,对促进晶体上皮细胞的增殖以及白内障术后囊混浊上起了重要作用。

By incorporation of tritiated thymidine or 5-bromodeoxyuridine and using them as the markers, we found that the basal cells, Clara cells and type II alveolar epithelial cells in the pulmonary tissues may in some way behave like stem cells and can eventually differentiate into respiratory tract epithelium.

通过应用cells in induced group cultured for 2 weeks 标记的胸腺嘧啶(3H-TdR)或5-溴脱氧尿核苷的渗入进行标记,发现肺组织的基底细胞、Clara 细胞和肺泡II型上皮细胞可能具有干细胞特征,能最终分化形成气道上皮。

By incorporation of tritiated thymidine or 5-bromodeoxyuridine and using them as the markers, we found that the basal cells, Clara cells and type Ⅱ alveolar epithelial cells in the pulmonary tissues may in some way behave like stem cells and can eventually differentiate into respiratory tract epithelium.

通过应用cells in induced group cultured for 2 weeks von kossa staining)标记的胸腺嘧啶(3H-TdR或5-溴脱氧尿核苷的渗入进行标记,发现肺组织的基底细胞、clara细胞和肺泡Ⅱ型上皮细胞可能具有干细胞特征,能最终分化形成气道上皮。

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