上皮细胞

The pured mammary epithelial cell line could be obtained by 2~3 passages. 2.Using RPMI1640 containing 10%FCS, 100IU/ml Penicillin and 100IU/ml Streptomycin as basic media, several different growth factors such as insulin, hydrocortisone, insulin-like growth factor, EGF and so on, were added and selected to culture goat mammary epithelial cells.

以RPMI1640 为基础培养液，山羊乳腺上皮细胞在含血清、双抗的同时，添加氢化考的松、Insulin、IGF-1、E2、EGF 及ITS 的不同组合，以细胞生长群体倍增值为参考标准，评估不同组合的生物活性因子对山羊乳腺上皮细胞生长的影响。

Three-dimensional Matrigel constructs contained aleolar forming units comprised of cells displaying AE2 cellular ultrastructure while expressing the SpC gene and gene product. The addition of tissue-specific growth factors induced formation of branching, sacculated epithelial structures reminiscent of the distal lung architecture.

通过对细胞亚微结构的观察和对SpC基因及其表达产物的分析，研究者发现培养体系中肺组织形成单位中具有能向肺泡II型上皮细胞分化的细胞；而通过添加组织特异性的生长因子，他们还发现这一做法会促进培养组织形成具有分支和囊状上皮结构的类似终末肺泡组织的形态结构。

Only weak expression of ICAM-1 was found on bronchial and alveolar epithelium, pulmonary arteriolar and venular endothelium in normal and sensitized rat lung. The expression of ICAM-1 in all tissues mentioned above were increased in asthmatic rats.

免疫组化结果显示，哮喘大鼠支气管上皮细胞、肺动静脉内皮细胞及肺泡上皮细胞ICAM-1表达均明显高于对照组。

MethodsHuman embryonic lens epithelial cells were cultured by the explanting culture methods. An inverted phase contrast microscope, electron microscope and immunocytochemistry were used to study the growth specialties of the human embryonic lens epithelial cells.

方法组织块贴壁法培养人胚晶状体上皮细胞，通过倒置相差显微镜、透射电子显微镜，采用免疫细胞化学法分析人胚晶状体上皮细胞的生长特性。

But the superficial epithelium was partially keratinized. Conclusion Tissue engineered gingiva may be constructed with human ginginval epithelia and ADM in vitro.

利用体外培养的牙龈上皮细胞和脱细胞真皮，可以在体外联合构建具有复层上皮细胞结构的组织工程牙龈。

The distribution of fibronectin in human oocyte and 2-cell to morulae embryos was also investigated, and the possible roles of fibronectin in fertilization, the development of early embryo and implantation were also discussed.

应用免疫组织化学方法和图像分析技术研究整合素β_3、FN和ER、PR在输卵管粘膜内的表达和变化；采用间接免疫荧光技术对FN在人卵母细胞和早期胚胎内的表达分布进行观察；光镜观察输卵管粘膜上皮细胞的形态学变化。结果：1。整合素β_3在人正常输卵管粘膜上皮纤毛细胞的胞浆内表达。

METHODS: Human bronchial epithelium cells were isolated and cultured from 18 patients undergone pueumonectomy in hospital. Interleukin 4 or interleukin 6 at concentration of 10 μg/L was used to treat these cultured cells, and Iamil was measured by whole cell patch clamp techniques.

体外分离培养气道上皮细胞，经过终浓度均为10 μg/L的IL-4、IL-6处理，用全细胞膜片钳技术记录原代培养的气道上皮细胞中Iamil。

Methods The Fas, FasL and DR4, DR5, TRAIL were assessed in 45 normal cervices, 52 cervical intraepithelial neoplasia gradeⅢ and 51 cervical cancers by immunohistochemical method. The number of apoptotic cells was determined by morphological criteria and the number of proliferating cells by counting Ki-67-positive cells. Results Proliferation and apoptosis percentage was markedly increased with increasing severity of neoplasia.

应用免疫组织化学法检测45例正常宫颈组织、52例宫颈上皮内瘤变Ⅲ级(cervical interaepithelial neoplasia Ⅲ， CINⅢ)和51例宫颈鳞状上皮细胞癌组织中Fas、FasL、DR4、DR5、TRAIL的表达，并以显微镜定位表达位置，以Ki-67阳性细胞记数增生细胞数。

Vitrectomy allows us to manage otherwise untreatable ocular pathologies, but the substitution of the vitreous by an artificial intraocular irrigation solution would probably disturb the physiology of the retina and choroids.

中文题名眼内灌注液对视网膜色素上皮细胞凋亡的影响副题名外文题名 The effects of different intraocular irrigating solutions on the apoptosis of RPE cells 论文作者殷春悦导师黎晓新教授学科专业眼科学研究领域\研究方向临床医疗技能训练与研究学位级别博士学位授予单位北京大学学位授予日期2001 论文页码总数94页关键词玻璃体切除术眼科手术细胞凋亡视网膜色素上皮细胞眼内灌注液馆藏号BSLW /2001 /R779.6 /42 随着玻璃体切除术的出现和日益发展，眼内灌注液作为术中使用的玻璃体替代物，直接影响视网膜和脉络膜的正常生理功能。

Desmosomal junctions were seen between cells. Conclusion The culture conditions used in the experiment are suitable for the primary culture of human laryngeal epithelial cells. The cultured cells will facilitate further research on etiology and pathogenesis of larynx carcinoma because of their proliferative and differentiative potency.

本实验采用的培养条件适宜人喉上皮细胞的生长，所获细胞纯度高，培养过程简便；从生物学行为来看，体外培养的人喉上皮细胞同样具有增殖能力和分化潜能，是进行癌变研究的良好对象。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

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