上皮细胞

Results Primary RPCs extracted from the neural retina demonstrate certain biological characteristics: the cell population could form neurospheres and younger adherent cells expanded in clusters.

结果 从兔胚胎视网膜神经部分离出的原代视网膜前体细胞具有明显的生物学特性：所培养细胞可形成悬浮生长的细胞团，刚贴壁的细胞成簇状生长，这些细胞用免疫细胞化学染色神经上皮干细胞蛋白表达呈阳性。

Glia; gliogenesis; glioma; glial cells; central nervous system; CNS; glial progenitor cells; glial fibrillary acidic protein; ependymocytes; ependoma; cytokeratins; vimentin; tanycytes; ion transport; lining cells; cerebrospinal fluid; CSF; ventricular system; floor plate; axon guidance; choroidal cells; choroid plexus epithelium; Chiari malformations; congenital aqueductal stenosis; neuroblast migration disorders; gap junctions

神经胶质；gliogenesis；神经胶质瘤；胶质细胞；中枢神经系统；CNS；神经胶质祖细胞；胶质原纤维酸性蛋白；室管膜细胞；ependoma；细胞角蛋白；波形蛋白；tanycytes；离子转运；衬细胞；脑脊髓液；CSF；脑室系统；底板；；脉络膜细胞；脉络丛上皮；恰里畸形；先天性导水管狭窄；成神经细胞迁徙扰乱；缺隙连接点

The expression of nestin antigen, NSE and GFAP were identified by immunohistochemistry, and the number of NSE-positive cells and GFAP-positive cells were dividedly counted.

结果大鼠胚胎神经管神经上皮干细胞在无血清培养基中可形成大量呈nestin抗原阳性细胞构成的神经球，经传代有血清培养后分化为NSE阳性和GFAP阳性细胞，其中NSE阳性细胞占细胞总数的47.7％，GFAP阳性细胞占细胞总数的39.8％。

Cases of tumor was performed by immunohistrochemical staining EMA and Vim were positive. 12 cases were hyperplasic lesions of mesothelial cell. Induced rats' MPM had a higher similarity with human' s MPM, especially in pathogenicity, positions, histological type and biological behavior of invasion and metastesis.

结果 实验组发生胸膜间皮瘤66例，诱发率71.74%，大体形态肉眼结节型间皮瘤较多见（72.73%）组织形态可见上皮型、梭形细胞型及混合型双向多潜能分化，13例免疫组化EMA及Vim双向阳性，并可见12例胸膜间皮细胞增生性病变，诱发瘤在病因，发病部位，组织形态学以及浸润转移的生物学行为方面与人体间皮瘤极为相似。

To reveal chemokines mediating the celiac metastasis of epithelial ovarian carcinoma by way of investigating the chemokine receptor expressions in serous epithelial ovarian carcinoma cell lines and tissues, and the corresponding ligand expressions in membrana abdominis.

本研究通过分析浆液性上皮性卵巢癌细胞系和组织趋化因子受体的表达，及腹膜间皮细胞相应配体的表达，以揭示趋化因子介导上皮性卵巢癌腹腔内转移机制。

We found that 1 mutant EG4 cells showed typical characteristics of pluripotent stem cells which had no obvious difference with wild cells; 2 When induced by 10〓 M retinoic acid , mutant EG4 cells differentiated into adipocytes with high frequency compared with mutant cells, suggested that EGFR plays a role in adipocyte differentiation; 3 when injected into nude mice, mutant teratocarcinomas contained a large amount of connective tissues as well as skeletal muscle, while wild EG4 cells produced frequently cartilage, keratinocyte and neuroepithelium.

我们建立了稳定表达胞内区功能缺失的外源EGFR cDNA片段的EG4细胞，分析其生长分化特性，发现 1）突变型细胞可在未分化状态下维持长时间的增殖，表明EGFR对EG多能干细胞表型无明显影响；2）〓 M的维甲酸A(retinoid acid A，RA)诱导后，大部分对照组细胞分化为脂肪细胞，而突变型细胞分化为脂肪细胞的比例明显较少，表明EGFR在脂肪细胞的发育分化中具有一定的调节作用；3）畸胎瘤切片分析显示，突变型瘤组织分布有大量的未分化细胞和结缔组织，分化细胞以肌肉细胞为主；对照组瘤组织含丰富的角质上皮、软骨、神经管等依赖EGFR的分化组织。

Comparing with control group, CD4~+ T cells of AIDS patient were markedly depleted in gastro-mucosal tissues(P＜0.01), but in presymptomatic patients CD4~+ T cells were not significantly different. CD3~+ T cells, CD8~+ T cells and NK. cells were notably increased (P.01), and they severely infiltrated glands and epitheliums in gastro-mucosal tissues. And the quantity and distribution of CD20~+ B cells between patients and control group were not obviously different.

HIV感染无症状者和AIDS患者胃黏膜活检组织中CD3~+T细胞、CD4~+T细胞、CD8~+T细胞、NK细胞、B细胞的数量及分布：与非HIV感染对照组相比，(1)AIDS患者胃黏膜内CD4~+T细胞显著减少(P＜0.01)，而无症状者与对照组无显著差异(P＞0.05)；(2)胃黏膜内CD3~+T细胞、CD8~+T细胞、NK细胞(CD57~+)显著增加(P＜0.01)，并呈现较明显普遍性噬胃黏膜上皮和腺体现象；(3)各组胃黏膜内CD20~+B淋巴细胞的数量分布变化不明显。3。

In pituitary and thyroid of kidney Yang deficiency group, there were ultrastructural damages of rough endoplasmic reticulum dilation, mitochondria vacuolation, cells transformation and nucleus pycnosis.

正常组肾上腺束状带细胞排列成束，细胞内含有大量的线粒体和大而圆的脂滴，睾丸曲细精管生精上皮依次排列着不同生精细胞-精原细胞、初级精母细胞、精细胞、精子。

Methods KC and FE of tympanic membrane were cultured on the surface of CCM prepared by torrefaction, then the cells biological characteristics were detected by immunofluorescence stain of keratin and vimentin, and the cells proliferative activity were identified by immunofluorescence stain of PCNA.

方法热干法制备CCM，传代的大鼠鼓膜上皮KC和FB分别接种于培养板及CCM表面，通过角蛋白、细胞膜波形蛋白免疫荧光染色鉴定细胞特异性，通过细胞核增殖抗原(proliferating Cell nuclear antigen， PCNA)免疫荧光染色测定膜上细胞的增殖能力，通过细胞培养液上清的经脯氨酸测定评价对FB胶原分泌功能的影响。

Methods The ARPE-19 cells were put into normal and hypoxic chamber respectively.After 0hr、8hr、24hr、48hr,the proliferation of ARPE-19 cells was evaluated by[3-(4,5-dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromid, MTT]-test.

第一部分缺氧人视网膜色素上皮细胞株ARPE-19表达特性目的观察缺氧对人视网膜色素上皮株ARPE-19的细胞形态、增殖以及对其分泌的血管内皮生长因子和色素上皮衍生因子表达的影响。

On the other hand, the more important thing is because the urban housing is a kind of heterogeneity products.

另一方面，更重要的是由于城市住房是一种异质性产品。

Climate histogram is the fall that collects place measure calm value, cent serves as cross axle for a few equal interval, the area that the frequency that the value appears according to place is accumulated and becomes will be determined inside each interval, discharge the graph that rise with post, also be called histogram.

气候直方图是将所收集的降水量测定值，分为几个相等的区间作为横轴，并将各区间内所测定值依所出现的次数累积而成的面积，用柱子排起来的图形，也叫做柱状图。