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Results:The results show that the survival rate of mammary epithelial cells were decreasing gradually with the increasing of the time of TGF-β1 treatment, and the survival rates of 24 h and 48 h groups were significantly lower than that of control group (p.05); the LDH activity of 6 h、12 h、24 h and 48 h groups were significantly higher than that of control group (p.05); the DNA degradation arisesed in 12 h, 24 h, and 48 h groups' mammary epithelial cells; the activity of Caspase-3 reach to a peak at 24 h, which was significantly higher than that of control group (p.05); the acinus of epithelial cells appears to break off and collapse with the increasing of treatment time, but the leydig structure keeps integrate;...

结果表明:1、5、10 ng/mL的TGF-β1均能抑制乳腺上皮细胞的增殖,且随浓度增加抑制作用加大,呈现剂量依赖性,其中10 ng/mL TGF-β1组与对照组差异显著(P.05);10 ng/mL TGF-β1作用于乳腺上皮细胞24 h,随作用时间延长细胞凋亡率逐渐增加,6 h、12 h和24 h组与对照组相比差异极显著(P.01);LDH活力也不断升高,6 h、12 h和24 h组与对照组差异显著(P.05);DNA随 TGF-β1作用时间的延长发生不同程度降解;TGF-β1作用2 h~6 h细胞大量表达HSP70,6 h达到高峰,而后下降,2~12 h的表达量均显著高于对照组(P.05)。结论:TGF-β1可以抑制奶牛乳腺上皮细胞的增殖,具有浓度依赖性。10 ng/mLTGF-β1。。。

By using the method of Western blot on fractions of nuclear and cytosol of primary cultured mantle cells and immunogold electron microscopy, we found out the completely different localization of these two proteins: CaM was localized both in cytoplasm and nuclear but CaLP was localized only in the cytoplasm.

通过对体外培养的珍珠贝外套膜上皮细胞核质分离后进行的Western blot分析和免疫电镜研究发现,CaM和CaLP的亚细胞定位是截然不同的:CaM分布于外套膜上皮细胞的细胞核与细胞质中,而CaLP却仅分布于外套膜上皮细胞的细胞质中。

All the patients had mucocutaneous pigmentation, gastrointestinal polyps, abdominal pain, anemia, and so on. All the patients were examined by endoscopy, polyps were removed by electrocautery snear under endoscopes, and 3 cases underwent enterotomy operation because of intestinal obstruction and intussusception.

结直肠癌组织和正常结肠黏膜广泛表达ER-β,正常结肠上皮主要呈核染,而结直肠癌组织主要为胞浆染色,ERβ在结肠上皮细胞表达,尤其在处于腺体底部的结肠隐窝大量表达,ERβ还可在内皮细胞和肌细胞上表达。

Results Over 90% of nasal and sinus cavity got clean within 1 to 2 weeks postoperatively. About 80% of patients had the De-mucous reaction from the 3rd to 10th week , such as mucous edema, vesicles, granulation tissue, mini-polyps, fibrous hyperplasia, and adhesion or regenerated diseases may appear in this stage. All of these diseases competed with mucous epithelization.

结果 90%以上的术腔在1~2周内清洁,80%以上的术腔在3~10周内有水肿、囊泡、肉芽、息肉生长和纤维结缔组织增生、粘连等去粘膜化反应或再生病变发生,并与上皮化呈竞争性生长。90%的术腔在经过恰当的处理后可完成上皮化,其中接近60%的术腔是在术后11~14周完成上皮化。

This result indicates that WT1 gene plays an important role in differentiation and development of fetal kidney and may be the factor that promotes metanephric blastemal cell to differentiate into epithelial cell.

结果显示小胎龄肾组织中WT1蛋白在胚基细胞和幼稚肾小球细胞核表达而大胎龄组肾组织中WT1在肾小管细胞胞浆表达,阳性率分别为57.1%(8/14)和46.2%(6/13),提示WT1基因在胚胎肾分化发育的过程中起着重要作用,WT1蛋白可能是促进后肾胚基细胞向上皮细胞分化的调控因子,其表达在时间上和空间上都受到严格的调控,WT1的表达异常可能导致胚基细胞分化停滞。17例肾母细胞瘤WT1蛋白表达阳性率为41.2%(7/17),阳性部位在胚基型和上皮型肿瘤细胞核,表达部位和阳性率与早期胚胎肾相似,其中间质型肾母细胞瘤均为阴性,胚基型和上皮型肾母细胞瘤阳性率70%(7/10),两组间阳性率有显著差异。

AbstractWe present the early diagnosis method of agerelated macular degeneration , and characterize various pathological changes arising in the development of AMD, such as drusen, chorioretinal mapshrinking, retinal pigment epithelial atrophy, choroidal neovascularization, retinal pigment epithelium and neural epithelial detachment, and so on. We analyze its images by using optical coherence tomography.

本文给出了一种老年性黄斑变性(agerelated macular degeneration, ARMD )的早期诊断方法,在 ARMD 的发展过程中出现的各种病理改变如玻璃膜疣、脉络膜视网膜地图状萎缩、视网膜色素上皮萎缩、脉络膜新生血管、视网膜色素上皮/神经上皮脱离等,用光学相干断层成像技术(optical coherence tomography,OCT)对其进行图像分析。

AbstractWe present the early diagnosis method of agerelated macular degeneration , and characterize various pathological changes arising in the development of AMD, such as drusen, chorioretinal mapshrinking, retinal pigment epithelial atrophy, choroidal neovascularization, retinal pigment epithelium and neural epithelial detachment, and so on.

本文给出了一种老年性黄斑变性(agerelated macular degeneration,ARMD)的早期诊断方法,在ARMD 的发展过程中出现的各种病理改变如玻璃膜疣、脉络膜视网膜地图状萎缩、视网膜色素上皮萎缩、脉络膜新生血管、视网膜色素上皮/神经上皮脱离等,用光学相干断层成像技术(optical coherence tomography,OCT)对其进行图像分析。

Although ERβ was also seen in these cells,there was irregularity of its distribution.Weak to moderate cytoplastic staining of ERβ in epithelial cells of lobules and ducts were fonud.

ERβ在小叶上皮细胞和导管上皮细胞的细胞核内检测到,但是ERβ在上皮细胞中的分布并无规律,而且在细胞浆内也可以检测到弱到中等强度的染色。

Methods The ARPE-19 cells were put into normal and hypoxic chamber respectively.After 0hr、8hr、24hr、48hr,the proliferation of ARPE-19 cells was evaluated by[3-(4,5-dimethylthiazole-2yl)-2,5-diphenyl tetrazolium bromid, MTT]-test.

第一部分缺氧人视网膜色素上皮细胞株ARPE-19表达特性目的观察缺氧对人视网膜色素上皮株ARPE-19的细胞形态、增殖以及对其分泌的血管内皮生长因子和色素上皮衍生因子表达的影响。

In this essay, the culture methods of corneal epithelial cell and keratocytes have been studied. The method of rabbit corneal epithelial cells has been improved in order to solve the problem that the cells moved slowly and could not form monolayer when the traditional tissue nubbles cultivation was adopted.

本文对兔角膜上皮细胞和基质细胞的培养方法进行了研究,解决了传统角膜上皮细胞组织块贴壁培养时细胞迁出慢,不易形成单层的难题,建立了兔角膜上皮细胞原代培养的方法。

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