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With the help of Axioplan 2 imaging system, we quantified the change of expression of ICAM-1, MUC5AC, goblet cell and NF-κB. Analyze the results by SAS method. Results (1) Grouped by index of lung function, the expression of ICAM-1 at the level of gene and protein had increased significantly in the epithelial cells of patients with COPD, compared with group of normal lung function.

取病灶远端的正常叶段支气管组织制备石蜡切片,原位杂交检测气道上皮中ICAM-1的mRNA的表达量;免疫组化检测气道上皮中ICAM-1和MUC5AC的蛋白表达变化;AB/PAS特殊染色法检测患者气道上皮细胞中杯状细胞的含量;PCR扩增法检测原代培养的气道上皮中MUC5AC的基因表达量和NF-κB的变化。

Grossly, the tumors ranged from 0.7 to 17 cm, and exhibited a spongy or bubble wrap appearance reflecting the microscopic presence of variably sized cystically dilated tubules lined by a single layer of epithelium. The lining varied with a cuboidal, flat, and hobnail cell appearance, and the neoplastic cells had abundant eosinophilic cytoplasm and enlarged nuclei with prominent nucleoli.

大体上,肿瘤大小为0.7至17cm,海绵样或泡沫样外观,镜下观是大小不等的扩张成囊的管子,这些管子被覆单层上皮,上皮形态立方状、扁平状和鞋钉样,肿瘤细胞胞浆丰富、嗜酸,细胞核大,核仁明显。

Results一. Culturing and identification of human conjunctiva cells1. morphous of conjunctiva cellsCells were harvested by tissue cultivation after digestion. Ponderosus cells adhered after 48 hours. Cells were shown in shape of round, ellipse and polygon. Cell body was loose and lucency with nucleus in center, cell membrane was also clearly seen. Cells were arranged in inlay and fuse in film after 12-14 days.2. Immunochemistry testNomogeneous immunochernical positive granules against CK13 were observed in endochylema of human conjunctiva cells.3. growth curve of conjunctival cellsSubcultured cells were aged and feeble after 5th passage.

结果一、正常人结膜细胞的培养与鉴定1、活体细胞的形态消化后组织块培养法:48小时后见大量上皮细胞贴壁,细胞不规则呈圆形、椭圆形、多边形,细胞体肥大透亮,细胞核位于中央,胞膜清楚,约12-14天后细胞呈镶嵌状排列,融合成膜状。2、免疫组化鉴定培养的人结膜上皮细胞CK13免疫组化染色见阳性颗粒均匀分布于胞浆中。3、培养结膜细胞生长曲线传代培养多数样本于P5代以后开始出现衰老征象。

Biopsies from confirmed EDS patients showed sloughing and flaking of superficial squamous epithelium with occasional bullous separation of the layers, parakeratosis, and varying degrees of acute or chronic inflammation.

这些明确的EDS病例组织学表现为表层鳞状上皮脱落或剥脱,偶见表皮内大疱形成,鳞状上皮角化不全,以及程度不一的急慢性炎细胞浸润。

Samples of the composites were taken for histological examination at day 7 and day 14 since OKCs were seeded. Results Multi-layer epithelia without hemidesmosome and basal membrane were observed in composites of PGA showing ill-defined cell layers. OKCs could adhere and proliferate onto collagen membrane forming multi-layer epithelia, with no mature hemidesmosome and basal membrane. That of Alloderm showed normal epithelial structures, with hemidesmosome, basal membrane and cell pseudopod in lamina propria.

结果 以PGA为支架构建的组织工程化口腔黏膜,含类似上皮层和固有层,但细胞层次不清;胶原膜上可见口腔黏膜上皮细胞黏附生长,并形成复层上皮样组织,但无成熟的桥粒及基底膜样结构;以Alloderm作为支架材料的构建物,出现正常上皮形态,含半桥粒及细胞指状突起等超微结构。

Part I Role of glycogen synthase kinase 3 in squamous differentiation induced by cigarette smoke in porcine airway epithelial cell Squamous differentiation of airway epithelium induced by diverse stimuli, such as cigarette smoke, has been thought to be an adaptive response to chronic injury as well as a precancerous lesion of lung squamous carcinoma.

第一部分糖原合成酶激酶3在吸烟诱导的猪气道上皮细胞鳞状分化中的作用研究气道上皮的鳞状细胞化生常见于慢性支气管炎症、致癌剂刺激等,一般被认为是对慢性损伤的一种适应性反应,也是肺鳞癌的癌前病变,其分子机制尚未完全阐明。

The expression of CD44 was strong positive in stratum intermedium at the bell of differetiational stage, bell of secretory stage, and bell of maturative stage of mouse tooth germ.

在钟状期CD44在口腔上皮、牙板上皮强阳性表达;外釉上皮、星网状层、成牙本质细胞弱阳性表达;分泌期的成釉细胞和牙囊阳性表达。

Barrett's oesophagus is a metaplastic change of the lining of the oesophagus, such that the normal squamous epithelium is replaced by specialised or intestinalised columnar epithelium.

Barrett食管是一种食管黏膜的化生改变,即正常鳞状上皮被特化或肠化的柱状上皮所取代。

Tu acetate rapidly after columnar epithelial edema, gray and showed typical "GRAPE BUNCH" change, squamous epithelium and the absence of such changes, the junction-scales become very slippery and energetic.

涂醋酸后柱状上皮迅速水肿、变白,呈典型的&葡萄串&改变,而鳞状上皮没有这种改变,鳞柱交界变得非常滑晰。

The number of clusters was automatically determined by Bayesian Information Criteria with log - likelihood distance measure.

结果对于正常、低度鳞状上皮内病变和高度鳞状上皮内病变宫颈细胞的分类正确率分别为98.0%。96.1%和100%。

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