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Briefly, under fluorescence microscopy, ingested ROS was shown by yellow fluorescence, which was a mixture of red and green, ROS just bounding to MSCs surface only revealed green.

三、检测分化的色素上皮样细胞的吞噬功能1、荧光标记法检测分化的色素上皮样细胞的吞噬功能参照Margaret等的双重荧光标记法。

The strong positive expression located in the gland alveolus and duct of serous gland, as well as myoepithelium of mucus glands. In some cases, blood vessel endothelium and fibrous connective tissue also show weakly positive expression of NF-κB.

NF-κB的表达主要见于浆液腺腺泡上皮和导管上皮,呈强阳性表达;黏液腺肌上皮样上皮呈强表达;间质成分中血管内皮呈弱阳性表达、纤维结缔组织呈弱阳性表达。

HPMCs from effluent derived of patients undergoing CAPD had markedly varied morphologic features, ranging from a cobblestone-like appearance similar to that of mesothelium derived from omentum to fibroblast-like cells or mixed cell populations.

2相差倒置显微镜观察新开管的患者腹透流出液培养的HPMCs形态上一小部分呈圆形,椭圆形的上皮样细胞,融合后呈铺路石状,与大网膜培养的腹膜间皮细胞相似;而大部分为上皮样细胞和梭形的纤维样细胞的混合存在;腹透一年以上的患者腹透流出液培养的HPMCs多为梭形的纤维样细胞。

Samples of the composites were taken for histological examination at day 7 and day 14 since OKCs were seeded. Results Multi-layer epithelia without hemidesmosome and basal membrane were observed in composites of PGA showing ill-defined cell layers. OKCs could adhere and proliferate onto collagen membrane forming multi-layer epithelia, with no mature hemidesmosome and basal membrane. That of Alloderm showed normal epithelial structures, with hemidesmosome, basal membrane and cell pseudopod in lamina propria.

结果 以PGA为支架构建的组织工程化口腔黏膜,含类似上皮层和固有层,但细胞层次不清;胶原膜上可见口腔黏膜上皮细胞黏附生长,并形成复层上皮样组织,但无成熟的桥粒及基底膜样结构;以Alloderm作为支架材料的构建物,出现正常上皮形态,含半桥粒及细胞指状突起等超微结构。

Methods One case of epithelioid hemangioendotheloma was observed on hi-stopathologic characteristics and immunohistochemical staining with review of the literatures.

观察和分析1例子宫上皮样血管内皮瘤的病理形态特征和免疫组化标记并结合文献复习总结子宫上皮样血管内皮瘤的临床病理学特点和鉴别诊断。

However, very few cases of ES have been studied, and INI1 expression in other epithelioid malignant neoplasms has not been examined systematically.

最近有研究表明,一些上皮样肉瘤的INI1基因也发生了失活,但这些研究中ES的病例很少,且也没有系统地检查INI1在其他上皮样恶性肿瘤中的表达情况。

Results Microscopically, the tumor cells were epithelioid or short spindled and special nests of tumor cells separated by arboring fibrovascular septa. Such morphological variants of CCSK as myxoid degeneration, microcysts formation and sclerosing pattern of extracellular hyalined collagen simulating osteoid were present in some regions.

结果 镜下见瘤细胞为上皮样或短梭形,被分枝状纤维血管间质分隔成巢团状,部分区域见黏液样变性微囊肿和细胞外胶原玻璃样变类似骨样组织的硬化型等形态变异。

We evaluated 350 tumors: 136 ES, including 64 conventional ES, 64 proximal-type ES, and 8 with hybrid features of conventional and proximal-type ES; 54 metastatic carcinomas (22 from lung, 6 breast, 6 stomach, 5 colorectum, 5 kidney, 5 prostate, 5 pancreas); 12 metastatic testicular embryonal carcinomas; 20 metastatic melanomas; 20 epithelioid mesotheliomas; 20 epithelioid angiosarcomas; 10 epithelioid hemangioendotheliomas; 24 epithelioid malignant peripheral nerve sheath tumors; 22 myoepithelial carcinomas of soft tissue; 7 anaplastic large cell lymphomas; 5 histiocytic sarcomas; and 10 control MRT of infancy (4 brain, 3 liver, 2 soft tissue, 1 kidney).

我们总共研究了350例肿瘤:136例ES,其中包括64例寻常型ES、64例近端型ES和8例混有前两型特征的混合型ES;54例转移癌(22例来自肺、6例来自乳腺、6例来自胃、5例来自结直肠、5例来着肾、5例来着前列腺、5例来着胰腺);12例转移性睾丸胚胎性癌;20例转移性黑色素瘤;20例上皮样间皮瘤;20例上皮样血管肉瘤;10例上皮样血管内皮瘤;24例上皮样恶性外周神经鞘膜瘤;22例软组织的肌上皮癌;7例间变性大细胞淋巴瘤;5例组织细胞肉瘤;10例作为对照的婴幼儿MRT(4例位于脑、3例位于肝、2例位于软组织、1例位于肾)。

There were all kinds of tissue cells in the teratomas acquired from in-vitro differentiation, such as, neuro epithelium, skeletal muscle, respiratory epithelium. This further proved the isolated mouse ES cells could differentiate into three germ layers of tissue types.

体内分化实验所获得的畸胎瘤中存在各种组织细胞,如一些神经上皮样组织;横纹肌;呼吸上皮样组织等,这进一步证明所分离获得的小鼠ES细胞具有三个胚层的分化能力。

The mitotic count was higher than 5/50HPF in 5 cases in which the diameters of tumor were above or equal to 5 cm. Cystification, hemorrage or necrosis were found in 4 cases and mucification in 1 case. The mitotic count was 2-5/50HPF in 2 cases which were epithelioid cell and tumor diameter were 2-4 cm with mucification in 1 case.

核分裂像大于5/50HPF的5例中肿瘤直径均大于或等于5 cm,其中4例伴有囊性变、出血或坏死,1例伴有黏液样变。2例核分裂像在2~5/50HPF者均为上皮样细胞型,其中1例伴有黏液样变,肿瘤直径均2~4 cm。

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