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3The quantitative analysis to IEL and GC shows that PO can increase the number of IEL in eccum and Sacculus Rotundus.

对兔肠道淋巴组织圆小囊圆顶区两侧上皮内淋巴细胞和杯状细胞及盲肠蚓突绒毛上皮内淋巴细胞的数量进行统计分析,结果发现,马齿苋提取液能提高圆小囊和盲肠蚓突的IEL的数量。

Result of HE and special dye1.1 result of HE More cells are found in subsequent cholesteatoma. Cholesterol crystal, both sequestrum and neogenesis bone , gland metaplasia, Polykaryocyte are found in subepidermis of subsequent cholesteatoma.

结果1、HE染色结果:可见后天性胆脂瘤上皮的细胞层次增多,上皮内及上皮下有不同程度的急性、慢性炎细胞浸涧;上皮下有时可见胆固醇肉芽肿、死骨与新骨形成、腺体化生等病理改变。

EGF was mainly located in the cytoplasm and extracellular matrix of intestinal villus cells, endothelial cells and tunica serosa epithelial cells, while EGFR chiefly distributed in the cellular membrane of these cells.

其中 EGF主要存在于小肠黏膜上皮细胞、黏膜下层的血管内皮细胞内和浆膜上皮细胞的胞浆和胞外基质中,EGFR则分布于这些细胞的细胞膜上。

Methods: Specimens of 46 cases of cervical cancer, 12 cases of normal cervical epithelia and 22 cases of cervical intraepithelial neoplasia were examined for E-cad expression by immunohistochemical staining.

应用SP免疫组化法观察12例正常宫颈上皮、46例宫颈鳞癌和22例宫颈上皮内瘤样病变组织中E-cad的表达。

PART 2:The molecular mechanisms of Nontypeable Haemophilus influenzae induced inflammatory response in respiratory epithelial cells Objective:Nontypeable Haemophilus influenzaewas traditionally considered as an extracellular pathogen in respiratory infections.In this study,we established a model of infected cells to confirm NTHI was also a facultatively intracellular pathogen.Mitogen activated protein kinasesare the important signal transduction pathway in mediating inflammatory response induced by NTHI in respiratory epithelium.In this study,we elucidate the impact of NTHI,and its SCF and EP on the expression of MAPK and subsequent proinflammatory mediators.

第二部分:NTHI致呼吸道上皮细胞炎症反应的分子机制研究目的:通过建立呼吸道上皮细胞株的体外NTHI感染模型,确认NTHI在细胞内的存活,观察NTHI及其组分(可溶性胞质成分SCF、外膜蛋白EP)对呼吸道上皮细胞MAPK信号传导通路和后续的前炎症因子表达的影响,并应用特异性ERK-MAPK抑制剂U0126、p38 MAPK抑制剂SB203580特异性阻断下游MAPK信号传导通路,观察它们对呼吸道上皮细胞分泌前炎症因子表达的影响。

Objective To explore the significance of DNA ploidy and cell proliferous ratio of prostate intraepithelial neoplasia in diagnose and differential diagnose.

目的探索DNA倍体类型、细胞周期在前列腺上皮内瘤中诊断和鉴别诊断的价值。方法应用细胞图像分析技术检测前列腺上皮内瘤细胞的平均DNA含量、DNA平均倍体值及细胞周期的各个时相的变化。

The distribution of fibronectin in human oocyte and 2-cell to morulae embryos was also investigated, and the possible roles of fibronectin in fertilization, the development of early embryo and implantation were also discussed.

应用免疫组织化学方法和图像分析技术研究整合素β_3、FN和ER、PR在输卵管粘膜内的表达和变化;采用间接免疫荧光技术对FN在人卵母细胞和早期胚胎内的表达分布进行观察;光镜观察输卵管粘膜上皮细胞的形态学变化。结果:1。整合素β_3在人正常输卵管粘膜上皮纤毛细胞的胞浆内表达。

In rat esophagus, the positive varicosities and fibres could be observed in the circular muscle layer and the muscularis mucosae layer at 21st day before birth. With the development of rat digestive tract, neurokinin A-immunoreactivity positive nerves could be observed gradually in the epithelium, submucosa, longitudinal muscle layer, myenteric plexus and submucosal plexes, while mature nerve fibres could be seen at one month after birth. 2. In rat stomach, the positive reaction of NKA initially happened in the myenteric plexus at the 14th day of embryo, and then appeared on circular muscles, longitudinal muscles, submucosal, muscularis mucosae, lamina propria and epithelium. 30 days after birth, expression of NKA is same as seen in adaulthood. 3. In rat small intestinal, the NKA-IR could first be found in the myenteric plexus of the duodenum, jejunum and ileum at 14th , 15th and 17th day before birth respectively. Then the NKA-IR occurred in the longitudinal muscle layer, circular muscle layer, intestinal villus, intestinal gland, muscularis mucosae, submucosa, submucosal plexes, mucosa plexus and deep muscular plexus.

结果 1、在食管,于胚胎21天的粘膜肌层和环肌层内观察到神经激肽A免疫阳性膨体纤维,出生后,随幼鼠的生长发育,相继在上皮,粘膜下层,纵肌层、肌间丛、粘膜下丛有NKA-IR表达,30天时已和成年鼠相似;2、在胃,首先于胚胎14天的肌间丛出现NKA-IR的表达,随发育相继在环肌、纵肌,粘膜下层、粘膜肌、固有膜及上皮内出现NKA-IR的表达,30天时具备成年鼠的分布特征;3、在小肠,分别于胚胎14、15、17天的十二指肠、空肠和回肠的肌间丛处出现NKA-IR的表达,随发育相继出现在纵肌、环肌、绒毛、小肠腺周、粘膜肌、粘膜下层、粘膜下丛、粘膜丛、深肌丛。

NGF positive immunoreactive cells were observed in the airway epithelia of control group. The positive reactivity are located at cell plasm. The NGF positive immunoreactive cells are round or polygonal shape and throughout the whole C〓~T〓 spinal ganglion of control group.

对照组豚鼠气道上皮内可见到含有NGF免疫反应阳性颗粒的上皮细胞,阳性反应物位于胞浆,呈棕褐色。C〓~T〓段脊神经节内,NGF免疫反应阳性细胞遍布整个神经节,多呈大圆形或多角形。

The number of clusters was automatically determined by Bayesian Information Criteria with log - likelihood distance measure.

结果对于正常、低度鳞状上皮内病变和高度鳞状上皮内病变宫颈细胞的分类正确率分别为98.0%。96.1%和100%。

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